T Cell Activation Bioassay (TCRαβ-KO), Propagation Model Technical Manual
Instructions for Use of Product(s)
GA1162, GA1172, GA1182, GA1210, GA1220, GA1230
Literature # TM653
The T Cell Activation Bioassay (TCRαβ-KO), Propagation Model, is a bioluminescent reporter cell-based assay that can be used to measure the potency of transgenic TCR constructs to activate T cells without the constraints of endogenous TCR expression. The assay consists of a genetically engineered Jurkat T cell line with endogenous TCR alpha and beta chains knocked out using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These cells also express a luciferase reporter driven by a TCR pathway-dependent promoter.
The TCRαβ-KO Cells are provided in cell propagation model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use. In addition, this assay is available in a cell bank format, which includes vials of TCRαβ-KO cells for customers that are planning to expand the CPM vials. We offer separately a TCR Transfection Positive Control Kit that includes a plasmid encoding a Positive Control TCR, a cognate Positive Control Peptide and APCs in thaw-and-use format.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Updated text about the label in Section 3.
2. Updated Lumit to a registered trademark.
3. Made miscellaneous text edits.
4. Removed an expired patent statement.
Revised 5/25.