IL-12 Bioassay, Propagation Model Technical Manual
Instructions for Use of Product(s)
J3042
Literature # TM588
The IL-12 Bioassay, Propagation Model is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells are provided in a Cell Propagation Model (CPM) format, as cryopreserved cells that can be thawed, propagated and banked for long-term use. This genetically engineered human cell line expresses a luciferase reporter driven by a response element (RE). When IL-12 binds to IL-12R it transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Updated the fonts and the cover image.
2. Revised text about the label in Section 3.
3. Removed an expired patent statement and updated another patent statement.
4. Made miscellaneous text edits.
Revised 7/25.