Rabbit Reticulocyte Lysate Technical Manual
Instructions for Use of Product(s)
L4960, L4151
Literature # TM232
Rabbit Reticulocyte Lysate Translation Systems are used in the identification of mRNA species, the characterization of their protein products and the investigation of transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. The reticulocytes are purified to remove contaminating cells, which could otherwise alter the translational properties of the final extract. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).
Rabbit Reticulocyte Lysate may contain a variety of post-translational processing activities, including acetylation, isoprenylation and some phosphorylation activity. Processing events such as signal peptide cleavage and core glycosylation can be examined by adding Canine Pancreatic Microsomal Membranes (Cat.# Y4041) to a standard translation reaction.
Summary of Change
The following changes were made to the 9/25 revision of this document:
1. Removed the discontinued Canine Pancreatic Microsomal Membranes, Cat. Y4041 from several sections.
2. Updated the cover image and fonts.
3. Updated storage temperature for Cat.# L4960 and Cat.# L4151 from –70°C to “below –65°C”.
4. Removed reference 3 and renumbered other references.
5. Removed an expired third-party trademark.
Revised 9/25.