Flexi® Vector Systems Technical Manual
Instructions for Use of Product(s)
C8640, C8820, C9320
Literature # TM254
The Flexi® Vector Systems use two rare-cutting restriction enzymes, SgfI and PmeI, in a simple, yet powerful, directional cloning method for protein-coding sequences. These systems provide a rapid, efficient and high-fidelity way to transfer these sequences between a variety of Flexi® Vectors. The Flexi® Vectors contain various expression or peptide tag options to enable expression of native or fusion proteins to study protein structure and function, as well as protein-protein interactions. All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.
Summary of Changes
The following changes were made to the 10/25 revision of this document:
1. Updated fonts.
2. Removed expired patent statements.
3. Updated the elution volume in Section 4.C, Step 6.
4. Notes were changed from numerical to alphabetical lists.
5. Updated Section 9.I. to add new and remove discontinued products.
6. Made miscellaneous minor text edits.
Revised 10/25.