Análisis de MSI en clínica para IVD

Since 1983, MSI screening has been recognized as important in the care of patients with colorectal cancer (CRC). High-frequency MSI (MSI-H) is recognized as a potential marker for germline mutations in certain DNA mismatch repair (MMR) genes associated with the hereditary cancer syndrome, Lynch syndrome.

Lynch syndrome is caused by autosomal dominant mutations to the major mismatch repair genes MLH1, MSH2, MSH6 or PMS2 as well as the EPCAM gene that inactivates MSH2. Most often associated with colorectal cancer, Lynch syndrome also increases the lifetime risk of several other cancers including endometrial, ovarian and stomach cancer.

Universal screening of all colorectal cancer tumors for MSI status is recommended in guidelines by numerous physician-led organizations. NCCN Clinical Practice Guidelines In Oncology (NCCN Guidelines®), developed by a panel of 32 leading cancer centers, recommend all tumors be screened by MSI by PCR and/or immunohistochemistry¹. Published guidelines for molecular biomarker testing for colorectal cancer tissues by the American Society for Clinical Pathology (ASCP), College of American Pathologists (CAP), Association for Molecular Pathology (AMP), and American Society of Clinical Oncology (ASCO) recommend MSI by PCR be performed either stepwise or  concurrently with IHC testing².

Learn more about MSI testing in the Microsatellite Instability Resource Center.

1. Referenced with permission from NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines®) for Genetic/Familial High-Risk Assessment: Colorectal V. 1.2022 © National Comprehensive Cancer Network, Inc, 2022. All rights reserved. Accessed July 18, 2022. To view most recent and complete version of the guideline, go online to nccn.org.
2. Sepulveda, A.R., et al. (2017) J. Mol. Diag. 19, 187–225.

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