We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

CytoTox-ONE™ Homogeneous Membrane Integrity Assay Technical Bulletin

Literature # TB306

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a homogeneous, fluorometric method for estimating the number of nonviable cells present in multiwell plates. Cell viability is most often defined based on cell membrane integrity and is most commonly measured by observing the exclusion of Trypan blue or other vital dyes. Measuring leakage of components from the cytoplasm into the surrounding culture medium has been widely accepted as a valid method to estimate the number of nonviable cells. The CytoTox-ONE™ Assay is a rapid, fluorescent measure of the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into resorufin. The CytoTox-ONE™ Reagent mix does not damage healthy cells; therefore, researchers can measure released LDH directly in assay wells containing a mixed population of viable and damaged cells.

Printed in USA. Revised 5/09.

 

PDF Icon Complete Protocol PDF (292kb)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.