Obtain a more complete picture of a drug's maximal action and mechanism of cell death. Several of Promega's cell-based assays allow multiplexing applications directly in the same sample well, for example, measure cytotoxicity and viability, then perform a luminescent caspase activity assay with the ApoTox-Glo™ Triplex Assay.
Results of the ApoTox-Glo™ Triplex Assay in 96-well format for suspension K562 cells treated for 4 hours with ionomycin.
Ionomycin treatment for 4 hours results in a dose-dependent decrease in viability and increase in cytotoxicity with no caspase-3/7 activation, which is consistent with primary necrosis.
Results of the ApoTox-Glo™ Triplex Assay in 1536-well format for suspension Jurkat cells treated for 24 hours with SAHA.
SAHA treatment of Jurkat cells for 24 hours results in a dose-dependent decrease in viability, increase in cytotoxicity, and an increase in caspase-3/7 activity consistent with apoptosis.