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Promega Corporation

Characterize Responses

Obtain a more complete picture of a drug's maximal action and mechanism of cell death. Several of Promega's cell-based assays allow multiplexing applications directly in the same sample well, for example, measure cytotoxicity and viability, then perform a luminescent caspase activity assay with the ApoTox-Glo™ Triplex Assay.

Results of the ApoTox-Glo™ Triplex Assay in 96-well format for suspension K562 cells treated for 4 hours with ionomycin.

Ionomycin treatment for 4 hours results in a dose-dependent decrease in viability and increase in cytotoxicity with no caspase-3/7 activation, which is consistent with primary necrosis.

Results of the ApoTox-Glo™ Triplex Assay in 1536-well format for suspension Jurkat cells treated for 24 hours with SAHA.

SAHA treatment of Jurkat cells for 24 hours results in a dose-dependent decrease in viability, increase in cytotoxicity, and an increase in caspase-3/7 activity consistent with apoptosis.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.