This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN31K Nluc CMV-neo Flexi(R) Vector sequence reference points: Base pairs 5128 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 NanoLuc® protein coding region 1065–1577 Linker 1578–1589 SgfI 1590–1597 Barnase coding region 1621–1956 PmeI 1958–1965 SV40 late poly(A) region 2117–2338 SV40 early enhancer/promoter 2437–2849 EM7 bacterial promoter 2863–2929 Neomycin phosphotransferase coding region 2943–3737 Synthetic poly(A) signal 3801–3849 ColE1-derived plasmid replication origin 4085–4121 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 1101 CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT 1151 GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA 1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC 1251 CCGTATGAAG GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT 1301 TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG ATCCTGCACT 1351 ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGAT CGACTATTTC 1401 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT 1451 AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA 1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC 1551 TGGCGGCTGT GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCCAT 1601 GGAATAAGTA AGGAATCCAC ATGGCACAGG TTATCAACAC GTTTGACGGG 1651 GTTGCGGATT ATCTTCAGAC ATATCATAAG CTACCTGATA ATTACATTAC 1701 AAAATCAGAA GCACAAGCCC TCGGCTGGGT GGCATCAAAA GGGAACCTTG 1751 CAGACGTCGC TCCGGGGAAA AGCATCGGCG GAGACATCTT CTCAAACAGG 1801 GAAGGCAAAC TCCCGGGCAA AAGCGGACGA ACATGGCGTG AAGCGGATAT 1851 TAACTATACA TCAGGCTTCA GAAATTCAGA CCGGATTCTT TACTCAAGCG 1901 ACTGGCTGAT TTACAAAACA ACGGACCATT ATCAGACCTT TACAAAAATC 1951 AGATAATGTT TAAACGAATT CGGGCTCGGT ACCCGGGGAT CCTCTAGAGT 2001 CGACCTGCAG GCATGCAAGC TGATCCGGCT GCTAACAAAG CCCGAAAGGA 2051 AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG 2101 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA 2151 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG 2201 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC 2251 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGATGTGGGA 2301 GGTTTTTTTA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGAATTTT 2351 AACAAAATAT TAACGCTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG 2401 CATCTGTGCG GTATTTCACA CCGCATACGC GGATCTGCGC AGCACCATGG 2451 CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG 2501 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 2551 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 2601 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 2651 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 2701 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 2751 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT 2801 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 2851 AGCTTAATTA ACTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG 2901 TATAATACGA CAAGGTGAGG AACTAAACCC AGGAGGCAGA TCATGATTGA 2951 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 3001 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 3051 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 3101 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 3151 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 3201 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 3251 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 3301 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 3351 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 3401 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 3451 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 3501 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 3551 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 3601 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 3651 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 3701 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 3751 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGATGGCCGC 3801 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA 3851 ATCGATAGCG ATAAGGATCC TCTTTGCGCT TGCGTTTTCC CTTGTCCAGA 3901 TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT CTGCGGACTG 3951 GCTTTCTACC CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 4001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 4051 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 4101 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 4151 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 4201 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 4251 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 4301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 4351 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 4401 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 4451 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 4501 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 4551 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 4601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4651 TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT CTTTTCTACG 4701 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 4751 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTATAG TCCGGAAATA 4801 CAGGAACGCA CGCTGGATGG CCCTTCGCTG GGATGGTGAA ACCATGAAAA 4851 ATGGCAGCTT CAGTGGATTA AGTGGGGGTA ATGTGGCCTG TACCCTCTGG 4901 TTGCATAGGT ATTCATACGG TTAAAATTTA TCAGGCGCGA TTGCGGCAGT 4951 TTTTCGGGTG GTTTGTTGCC ATTTTTACCT GTCTGCTGCC GTGATCGCGC 5001 TGAACGCGTT TTAGCGGTGC GTACAATTAA GGGATTATGG TAAATCCACT 5051 TACTGTCTGC CCTCGTAGCC ATCGAGATAA ACCGCAGTAC TCCGGCCACG 5101 ATGCGTCCGG CGTAGAGGAT CGAGATCT Sequence file created 5-December-2013.