pFC15A (HaloTag(R) 7) CMVd1 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC15A (HaloTag(R) 7) CMVd1 Flexi(R) Vector sequence reference points: Base pairs 4182 CMVd1 intermediate early enhancer/promoter 1-121 T7 RNA polymerase promoter (-17 to +3) 132-151 SP6 RNA polymerase promoter (-17 to +3) 156-175 SgfI region 182-189 barnase coding region 213-548 EcoICRI region 568-573 TEV protease recognition sequence 588-608 HaloTag(R) 7 coding region 618-1508 SV40 late polyadenylation signal 1642-1863 Beta-lactamase (Ampr) coding region 2124-2984 ColE1-derived plasmid origin of replication 3139-3175 Cer region (site for E. coli XerCD recombinase) 3846-4131 .. 1 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAATAACCCC GCCCCGTTGA 51 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 101 GGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTAATACGAC TCACTATAGG 151 GTACGATTTA GGTGACACTA TAGAATAAGG AGCGATCGCC ATGGAATAAG 201 TAAGGAATCC ACATGGCACA GGTTATCAAC ACGTTTGACG GGGTTGCGGA 251 TTATCTTCAG ACATATCATA AGCTACCTGA TAATTACATT ACAAAATCAG 301 AAGCACAAGC CCTCGGCTGG GTGGCATCAA AAGGGAACCT TGCAGACGTC 351 GCTCCGGGGA AAAGCATCGG CGGAGACATC TTCTCAAACA GGGAAGGCAA 401 ACTCCCGGGC AAAAGCGGAC GAACATGGCG TGAAGCGGAT ATTAACTATA 451 CATCAGGCTT CAGAAATTCA GACCGGATTC TTTACTCAAG CGACTGGCTG 501 ATTTACAAAA CAACGGACCA TTATCAGACC TTTACAAAAA TCAGATAATG 551 TTTAATGACC CCGTGTCGAG CTCTCGAGCC AACCACTGAG GATCTGTACT 601 TTCAGAGCGA TAACGATGGA TCCGAAATCG GTACTGGCTT TCCATTCGAC 651 CCCCATTATG TGGAAGTCCT GGGCGAGCGC ATGCACTACG TCGATGTTGG 701 TCCGCGCGAT GGCACCCCTG TGCTGTTCCT GCACGGTAAC CCGACCTCCT 751 CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC CCATCGCTGC 801 ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG 851 TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC 901 TGGGTCTGGA AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG 951 GGTTTCCACT GGGCCAAGCG CAATCCAGAG CGCGTCAAAG GTATTGCATT 1001 TATGGAGTTC ATCCGCCCTA TCCCGACCTG GGACGAATGG CCAGAATTTG 1051 CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG CCGCAAGCTG 1101 ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT 1151 CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA 1201 ATCCTGTTGA CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC 1251 GCCGGTGAGC CAGCGAACAT CGTCGCGCTG GTCGAAGAAT ACATGGACTG 1301 GCTGCACCAG TCCCCTGTCC CGAAGCTGCT GTTCTGGGGC ACCCCAGGCG 1351 TTCTGATCCC ACCGGCCGAA GCCGCTCGCC TGGCCAAAAG CCTGCCTAAC 1401 TGCAAGGCTG TGGACATCGG CCCGGGTCTG AATCTGCTGC AAGAAGACAA 1451 CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCT ACTCTGGAGA 1501 TTTCCGGTTA ATAGAATTCT AGAGTCGACC TGCAGGCATG CAAGCTGATC 1551 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT 1601 GAGCAATAAC TAGCATAACC CCTTGGGGCG GCCGCTTCGA GCAGACATGA 1651 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA 1701 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 1751 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT 1801 TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTTAAGCAA GTAAAACCTC 1851 TACAAATGTG GTAAAATCGA ATTCTAATGG ATCCTCTTTG CGCTTGCGTT 1901 TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC 1951 GTTTCTGCGG ACTGGCTTTC TACGTAATGG TTTCTTAGAC GTCAGGTGGC 2001 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 2051 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 2101 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC 2151 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 2201 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 2251 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 2301 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTCAAAGTTC TGCTATGTGG 2351 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 2401 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG 2451 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 2501 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACT ATCGGAGGAC 2551 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 2601 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 2651 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 2701 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG 2751 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 2801 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 2851 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 2901 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 2951 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAATTCGAA ATGACCGACC 3001 AAGCGACGCC CAACCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 3051 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 3101 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 3151 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 3201 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 3251 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 3301 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 3351 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 3401 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 3451 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 3501 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 3551 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 3601 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 3651 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 3701 CAGATTACGC GCAGAAAAAA AGGATTTCAA GAAGATCCTT TGATCTTTTC 3751 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 3801 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT ATAGTCCGGA 3851 AATACAGGAA CGCACGCTGG ATGGCCCTTC GCTGGGATGG TGAAACCATG 3901 AAAAATGGCA GCTTCAGTGG ATTAAGTGGG GGTAATGTGG CCTGTACCCT 3951 CTGGTTGCAT AGGTATTCAT ACGGTTAAAA TTTATCAGGC GCGATTGCGG 4001 CAGTTTTTCG GGTGGTTTGT TGCCATTTTT ACCTGTCTGC TGCCGTGATC 4051 GCGCTGAACG CGTTTTAGCG GTGCGTACAA TTAAGGGATT ATGGTAAATC 4101 CACTTACTGT CTGCCCTCGT AGCCATCGAG ATAAACCGCA GTACTCCGGC 4151 CACGATGCGT CCGGCGTAGA GGATCGAGAT CT Vector sequence prepared 28-Jan-08.