pALTER(R)-MAX Vector(a,b,c) This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pALTER(R)-MAX Vector sequence reference points: Base pairs 5534 CMV immediate-early enhancer 1-659 CMV immediate-early promoter 669-750 Chimeric intron 890-1022 T7 RNA polymerase promoter (-17 to +2) 1067-1085 T7 RNA polymerase transcription initiation site 1084 Multiple cloning region 1091-1137 T3 RNA polymerase promoter (-16 to +3) 1144-1162 T3 RNA polymerase transcription initiation site 1146 SV40 late polyadenylation signal 1171-1392 f1 origin 1487-1942 SV40 enhancer/early promoter 2007-2377 Minimal SV40 origin of replication 2273-2338 Chloramphenicol acetyltransferase gene 2623-3281 Synthetic poly(a) 3379-3427 Beta-lactamase gene (AmpR)* 3846-4706 Ampicillin Repair Oligonucleotide binding site 4376-4398 Chloramphenicol Repair Oligonucleotide binding site 2827-2853 T7 EEV (forward) sequencing primer binding site** 1053-1074 T3 (reverse) sequencing primer binding site 1143-1162 Transcription start 2312,2318,2323 pALTER(R)-MAX and pALTER(R) are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office. (a)U.S. Pat. No. 5,955,363 has been issued to Promega Corporation for a vector for in vitro mutagenesis and use thereof. (b)The CMV promoter and its use are covered under U.S. Pat. Nos. 5,168,062 and 5,385,839 owned by the University of Iowa Research Foundation, Iowa City, Iowa, and licensed FOR RESEARCH USE ONLY. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation. (c)U.S. Pat. No. 4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted. *As the vector is supplied, this antibiotic resistance gene is inactivated. It can be reactivated by using one of the mutagenesis oligonucleotides provided. **The T7 EEV (Eukaryotic Expression Vector) Promoter Primer (Cat.# Q6700) is designed specifically to prime sequencing reactions from the mammalian expression vectors pCI-neo(b,c) (Cat.# E1841), pCI(b) (Cat.# E1731), pSI (Cat.# E1721) and pALTERŪ-MAX (Cat.# Q5761). 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAACAACTG CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG 701 TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC 751 ACTAGAAGCT TTATTGCGGT AGTTTATCAC AGTTAAATTG CTAACGCAGT 801 CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT GACTCTCTTA 851 AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA 901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC 951 AGAGAAGACT CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC 1001 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGTTCA ATTACAGCTC 1051 TTAAGGCTAG AGTACTTAAT ACGACTCACT ATAGGCTAGC CTCGAGAATT 1101 CACGCGTGGT ACCTCTAGAG TCGACCCGGG CGGCCGCAAA GTTCCCTTTA 1151 GTGAGGGTTA ATGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG 1201 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT 1251 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT 1301 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGATGT 1351 GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCCGA 1401 TAAGGATCGA TCCGGGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC 1451 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGACGC GCCCTGTAGC 1501 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 1551 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 1601 ACGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 1651 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 1701 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 1751 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 1801 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 1851 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 1901 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCTGATGC 1951 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATACGCGGA 2001 TCTTCCGTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG 2051 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 2101 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 2151 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 2201 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 2251 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 2301 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 2351 GGAGGCCTAG GCTTTTGCAA AAAGCTTGAT TCTTCTGACA CAACAGTCTC 2401 GAACTTAAGG CTAGCGAATA AATACCTGTG ACGGAAGATC ACTTCGCAGA 2451 ATAAATAAAT CCTGGTGTCC CTGTTGATAC CGGGAAGCCC TGGGCCAACT 2501 TTTGGCGAAA GTGAGACGTT GATCGGCACG TAAGAGGTTC CAACTTTCAC 2551 CATAATCAAA TAAGATCACT ACCGGGCGTA TTTTTTGAGT TATCGAGATT 2601 TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA 2651 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG 2701 TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC 2751 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA 2801 TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAACT CCGTATGGCA 2851 ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC 2901 CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC 2951 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT 3001 TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT 3051 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG 3101 TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT 3151 TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA 3201 TGCCGTTTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC 3251 AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AATTTTTTTA AGGCAGTTAT 3301 TGGTGCCCTT AAACGCCTGG TTGCTACGCC TGAATAAGTG ATAATAAGCG 3351 GATGAATGGC AGAAATTCGT CGAAGCGCAA TAAAATATCT TTATTTTCAT 3401 TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGATAGCGAT AAGGATCGGA 3451 AGATCCGCGT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG 3501 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 3551 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA 3601 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA 3651 AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 3701 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 3751 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 3801 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 3851 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 3901 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA 3951 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 4001 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 4051 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG 4101 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 4151 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 4201 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA 4251 CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 4301 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 4351 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCGCA ATGGCAACAA 4401 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 4451 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 4501 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 4551 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 4601 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 4651 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 4701 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 4751 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 4801 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 4851 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 4901 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 4951 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 5001 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 5051 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 5101 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 5151 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 5201 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 5251 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 5301 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 5351 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 5401 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 5451 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 5501 TGCTGGCCTT TTGCTCACAT GGCTCGACAG ATCT Sequence file updated 16-Jul-01.