pFN28K HaloTag(R) CMV-neo Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN28K HaloTag(R) CMV-neo Flexi(R) Vector sequence reference points: Base pairs 5535 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 HaloTag 7 N terminal region 1067-1957 HaloTag linker 1958-1996 TEV protease recognition region 1970-1990 Sgf I region 1997-2004 Barnase coding region 2028-2363 Pme I region 2365-2372 SV40 late poly(A) region 2524-2745 SV40 enhancer and early promoter 2844-3262 EM7 bacterial promoter 3270-3336 Neomycin phosphotransferase gene 3350-4144 Synthetic polyadenylation region 4208-4256 ColE1-derived plasmid origin of replication 4492-4528 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC 1101 CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT 1151 CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC 1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA 1251 TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT 1301 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT 1351 GGGTCTGGAA GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG 1401 GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT 1451 ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC 1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA 1551 TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC 1601 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA 1651 TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG 1701 CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG 1751 CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT 1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT 1851 GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC 1901 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT 1951 TTCCGGCGAG CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACGCGA 2001 TCGCCATGGA ATAAGTAAGG AATCCACATG GCACAGGTTA TCAACACGTT 2051 TGACGGGGTT GCGGATTATC TTCAGACATA TCATAAGCTA CCTGATAATT 2101 ACATTACAAA ATCAGAAGCA CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG 2151 AACCTTGCAG ACGTCGCTCC GGGGAAAAGC ATCGGCGGAG ACATCTTCTC 2201 AAACAGGGAA GGCAAACTCC CGGGCAAAAG CGGACGAACA TGGCGTGAAG 2251 CGGATATTAA CTATACATCA GGCTTCAGAA ATTCAGACCG GATTCTTTAC 2301 TCAAGCGACT GGCTGATTTA CAAAACAACG GACCATTATC AGACCTTTAC 2351 AAAAATCAGA TAATGTTTAA ACGAATTCGG GCTCGGTACC CGGGGATCCT 2401 CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA TCCGGCTGCT AACAAAGCCC 2451 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA 2501 CCCCTTGGGG CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT 2551 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 2601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA 2651 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA 2701 TGTGGGAGGT TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 2751 GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCTGATGC GGTATTTTCT 2801 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATACGCGGA TCTGCGCAGC 2851 ACCATGGCCT GAAATAACCT CTGAAAGAGG AACTTGGTTA GGTACCTTCT 2901 GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 2951 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 3001 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 3051 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 3101 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 3151 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT 3201 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 3251 TGCAAAAAGC TTAATTAACT GTTGACAATT AATCATCGGC ATAGTATATC 3301 GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCCAGG AGGCAGATCA 3351 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 3401 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC 3451 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 3501 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 3551 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT 3601 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 3651 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 3701 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA 3751 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 3801 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 3851 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT 3901 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 3951 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 4001 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG 4051 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 4101 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 4151 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA 4201 TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT 4251 TGTGTGAATC GATAGCGATA AGGATCCTCT TTGCGCTTGC GTTTTCCCTT 4301 GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG 4351 CGGACTGGCT TTCTACCCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 4401 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 4451 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 4501 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 4551 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 4601 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 4651 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 4701 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 4751 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 4801 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 4851 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 4901 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 4951 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 5001 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 5051 CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT 5101 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 5151 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC 5201 GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC 5251 ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC 5301 CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG 5351 CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG 5401 ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA 5451 ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC 5501 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCT Sequence file prepared 25-June-2011.