This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLF1-C [CMV/Hygro] Vector sequence reference points: Base pairs 5964 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter 1033–1052 Multiple cloning region 1052–1126 Linker 1127–1138 NanoLuc® protein coding region 1139–1648 SV40 late poly(A) region 1773–1994 SV40 early enhancer/promoter 2093–2505 Synthetic hygromycin (Hygr) coding region 2536–3573 Synthetic poly(A) signal 3597–3645 β-lactamase (Ampr) coding region 3906–4766 ColE1-derived plasmid replication origin 4921–4957 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCGATCGCTT CCGAATTCCT ACCGCGGATA TCTAGATTTG 1101 GGCCCAATTC CTGCAGGCGA GCTCTCGGCT CGAGCGGCGT CTTCACACTC 1151 GAAGATTTCG TTGGGGACTG GCGACAGACA GCCGGCTACA ACCTGGACCA 1201 AGTCCTTGAA CAGGGAGGTG TGTCCAGTTT GTTTCAGAAT CTCGGGGTGT 1251 CCGTAACTCC GATCCAAAGG ATTGTCCTGA GCGGTGAAAA TGGGCTGAAG 1301 ATCGACATCC ATGTCATCAT CCCGTATGAA GGTCTGAGCG GCGACCAAAT 1351 GGGCCAGATC GAAAAAATTT TTAAGGTGGT GTACCCTGTG GATGATCATC 1401 ACTTTAAGGT GATCCTGCAC TATGGCACAC TGGTAATCGA CGGGGTTACG 1451 CCGAACATGA TCGACTATTT CGGACGGCCG TATGAAGGCA TCGCCGTGTT 1501 CGACGGCAAA AAGATCACTG TAACAGGGAC CCTGTGGAAC GGCAACAAAA 1551 TTATCGACGA GCGCCTGATC AACCCCGACG GCTCCCTGCT GTTCCGAGTA 1601 ACCATCAACG GAGTGACCGG CTGGCGGCTG TGCGAACGCA TTCTGGCGTA 1651 AGGCCGCGAC TCTAGGGCAT GCAAGCTGAT CCGGCTGCTA ACAAAGCCCG 1701 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC 1751 CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT 1801 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA 1851 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA 1901 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGAT 1951 GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 2001 AATTTTAACA AAATATTAAC GCTTACAATT TCCTGATGCG GTATTTTCTC 2051 CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGCGGAT CTGCGCAGCA 2101 CCATGGCCTG AAATAACCTC TGAAAGAGGA ACTTGGTTAG GTACCTTCTG 2151 AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT 2201 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 2251 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 2301 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 2351 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 2401 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC 2451 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 2501 GCAAAAAGCT CGATTCTTCT GACACTAGCG CCACCATGAA GAAGCCCGAA 2551 CTCACCGCTA CCAGCGTTGA AAAATTTCTC ATCGAGAAGT TCGACAGTGT 2601 GAGCGACCTG ATGCAGTTGT CGGAGGGCGA AGAGAGCCGA GCCTTCAGCT 2651 TCGATGTCGG CGGACGCGGC TATGTACTGC GGGTGAATAG CTGCGCTGAT 2701 GGCTTCTACA AAGACCGCTA CGTGTACCGC CACTTCGCCA GCGCTGCACT 2751 ACCCATCCCC GAAGTGTTGG ACATCGGCGA GTTCAGCGAG AGCCTGACAT 2801 ACTGCATCAG TAGACGCGCC CAAGGCGTTA CTCTCCAAGA CCTCCCCGAA 2851 ACAGAGCTGC CTGCTGTGTT ACAGCCTGTC GCCGAAGCTA TGGATGCTAT 2901 TGCCGCCGCC GACCTCAGTC AAACCAGCGG CTTCGGCCCA TTCGGGCCCC 2951 AAGGCATCGG CCAGTACACA ACCTGGCGGG ATTTCATTTG CGCCATTGCT 3001 GATCCCCATG TCTACCACTG GCAGACCGTG ATGGACGACA CCGTGTCCGC 3051 CAGCGTAGCT CAAGCCCTGG ACGAACTGAT GCTGTGGGCC GAAGACTGTC 3101 CCGAGGTGCG CCACCTCGTC CATGCCGACT TCGGCAGCAA CAACGTCCTG 3151 ACCGACAACG GCCGCATCAC CGCCGTAATC GACTGGTCCG AAGCTATGTT 3201 CGGGGACAGT CAGTACGAGG TGGCCAACAT CTTCTTCTGG CGGCCCTGGC 3251 TGGCTTGCAT GGAGCAGCAG ACTCGCTACT TCGAGCGCCG GCATCCCGAG 3301 CTGGCCGGCA GCCCTCGTCT GCGAGCCTAC ATGCTGCGCA TCGGCCTGGA 3351 TCAGCTCTAC CAGAGCCTCG TGGACGGCAA CTTCGACGAT GCTGCCTGGG 3401 CTCAAGGCCG CTGCGATGCC ATCGTCCGCA GCGGGGCCGG CACCGTCGGT 3451 CGCACACAAA TCGCTCGCCG GAGCGCAGCC GTATGGACCG ACGGCTGCGT 3501 CGAGGTGCTG GCCGACAGCG GCAACCGCCG GCCCAGTACA CGACCGCGCG 3551 CTAAGGAGGT AGGTCGAGTT TAGACTCTAG CCATCACGAT GGCCGCAATA 3601 AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG 3651 ATAGCGATAA GGATCCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC 3701 CCAGTAGCTG ACATTCATCC GGGGTCAGCA CCGTTTCTGC GGACTGGCTT 3751 TCTACGTAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 3801 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 3851 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 3901 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG 3951 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 4001 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 4051 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 4101 ATGATGAGCA CTTTCAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 4151 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 4201 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG 4251 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 4301 GGCCAACTTA CTTCTGACAA CTATCGGAGG ACCGAAGGAG CTAACCGCTT 4351 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 4401 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 4451 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 4501 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA 4551 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 4601 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 4651 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 4701 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 4751 GATTAAGCAT TGGTAATTCG AAATGACCGA CCAAGCGACG CCCAACCGGT 4801 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 4851 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 4901 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 4951 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 5001 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 5051 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 5101 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 5151 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 5201 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 5251 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 5301 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 5351 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 5401 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 5451 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 5501 AAAGGATTTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 5551 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 5601 GGATCTTCAC CTAGATCCTT TTATAGTCCG GAAATACAGG AACGCACGCT 5651 GGATGGCCCT TCGCTGGGAT GGTGAAACCA TGAAAAATGG CAGCTTCAGT 5701 GGATTAAGTG GGGGTAATGT GGCCTGTACC CTCTGGTTGC ATAGGTATTC 5751 ATACGGTTAA AATTTATCAG GCGCGATTGC GGCAGTTTTT CGGGTGGTTT 5801 GTTGCCATTT TTACCTGTCT GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG 5851 CGGTGCGTAC AATTAAGGGA TTATGGTAAA TCCACTTACT GTCTGCCCTC 5901 GTAGCCATCG AGATAAACCG CAGTACTCCG GCCACGATGC GTCCGGCGTA 5951 GAGGATCGAG ATCT Sequence file created 5-December-2013.