This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC32K Nluc CMV-neo Flexi(R) Vector sequence reference points: Base pairs 5119 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 SgfI 1056–1063 Barnase coding region 1087–1422 EcoICRI 1442–1447 Linker 1447–1461 NanoLuc® protein coding region 1462–1971 SV40 late poly(A) region 2108–2329 SV40 early enhancer/promoter 2428–2840 EM7 bacterial promoter 2854–2920 Neomycin phosphotransferase coding region 2934–3728 Synthetic poly(A) signal 3792–3840 ColE1-derived plasmid replication origin 4076–4112 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 GCTCGAGCGG CGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG 1501 ACAGCCGGCT ACAACCTGGA CCAAGTCCTT GAACAGGGAG GTGTGTCCAG 1551 TTTGTTTCAG AATCTCGGGG TGTCCGTAAC TCCGATCCAA AGGATTGTCC 1601 TGAGCGGTGA AAATGGGCTG AAGATCGACA TCCATGTCAT CATCCCGTAT 1651 GAAGGTCTGA GCGGCGACCA AATGGGCCAG ATCGAAAAAA TTTTTAAGGT 1701 GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG CACTATGGCA 1751 CACTGGTAAT CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG 1801 CCGTATGAAG GCATCGCCGT GTTCGACGGC AAAAAGATCA CTGTAACAGG 1851 GACCCTGTGG AACGGCAACA AAATTATCGA CGAGCGCCTG ATCAACCCCG 1901 ACGGCTCCCT GCTGTTCCGA GTAACCATCA ACGGAGTGAC CGGCTGGCGG 1951 CTGTGCGAAC GCATTCTGGC GTAAGGCCGC GACTCTAGAG TCGACCTGCA 2001 GGCATGCAAG CTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT 2051 GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCGGCCG 2101 CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC 2151 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG 2201 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT 2251 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGATGTGGG AGGTTTTTTT 2301 AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGAATTT TAACAAAATA 2351 TTAACGCTTA CAATTTCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 2401 GGTATTTCAC ACCGCATACG CGGATCTGCG CAGCACCATG GCCTGAAATA 2451 ACCTCTGAAA GAGGAACTTG GTTAGGTACC TTCTGAGGCG GAAAGAACCA 2501 GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 2551 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 2601 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 2651 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 2701 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 2751 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA 2801 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTTAATT 2851 AACTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG 2901 ACAAGGTGAG GAACTAAACC CAGGAGGCAG ATCATGATTG AACAAGATGG 2951 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG 3001 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 3051 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 3101 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 3151 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 3201 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 3251 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC 3301 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 3351 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 3401 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 3451 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 3501 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 3551 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT 3601 TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 3651 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 3701 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT 3751 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGATGGCCG CAATAAAATA 3801 TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG AATCGATAGC 3851 GATAAGGATC CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT 3901 AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 3951 CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 4001 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4051 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 4101 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 4151 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 4201 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 4251 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 4301 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4351 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 4401 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 4451 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 4501 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 4551 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 4601 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4651 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 4701 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 4751 AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT ACAGGAACGC 4801 ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA AATGGCAGCT 4851 TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG GTTGCATAGG 4901 TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4951 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT 5001 TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG 5051 CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG 5101 GCGTAGAGGA TCGAGATCT Sequence file created 5-December-2013.