pFC17K (HaloTag(R) 7) CMVd3 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC17K (HaloTag(R) 7) CMVd3 Flexi(R) Vector sequence reference points: Base pairs 4122 CMVd3 intermediate early enhancer/promoter 1-66 T7 RNA polymerase promoter (-17 to +3) 77-96 SP6 RNA polymerase promoter (-17 to +3) 101-120 SgfI region 127-134 barnase coding region 158-493 EcoICRI region 513-518 TEV protease recognition sequence 533-553 HaloTag(R) 7 coding region 563-1453 SV40 late polyadenylation signal 1587-1808 Kanamycin resistance (Kanr) coding region 2116-2910 ColE1-derived plasmid origin of replication 3079-3115 Cer region (site for E. coli XerCD recombinase) 3786-4071 .. 1 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 51 AGTGAACCGT CAGATCACTA GAAGCTTAAT ACGACTCACT ATAGGGTACG 101 ATTTAGGTGA CACTATAGAA TAAGGAGCGA TCGCCATGGA ATAAGTAAGG 151 AATCCACATG GCACAGGTTA TCAACACGTT TGACGGGGTT GCGGATTATC 201 TTCAGACATA TCATAAGCTA CCTGATAATT ACATTACAAA ATCAGAAGCA 251 CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG AACCTTGCAG ACGTCGCTCC 301 GGGGAAAAGC ATCGGCGGAG ACATCTTCTC AAACAGGGAA GGCAAACTCC 351 CGGGCAAAAG CGGACGAACA TGGCGTGAAG CGGATATTAA CTATACATCA 401 GGCTTCAGAA ATTCAGACCG GATTCTTTAC TCAAGCGACT GGCTGATTTA 451 CAAAACAACG GACCATTATC AGACCTTTAC AAAAATCAGA TAATGTTTAA 501 TGACCCCGTG TCGAGCTCTC GAGCCAACCA CTGAGGATCT GTACTTTCAG 551 AGCGATAACG ATGGATCCGA AATCGGTACT GGCTTTCCAT TCGACCCCCA 601 TTATGTGGAA GTCCTGGGCG AGCGCATGCA CTACGTCGAT GTTGGTCCGC 651 GCGATGGCAC CCCTGTGCTG TTCCTGCACG GTAACCCGAC CTCCTCCTAC 701 GTGTGGCGCA ACATCATCCC GCATGTTGCA CCGACCCATC GCTGCATTGC 751 TCCAGACCTG ATCGGTATGG GCAAATCCGA CAAACCAGAC CTGGGTTATT 801 TCTTCGACGA CCACGTCCGC TTCATGGATG CCTTCATCGA AGCCCTGGGT 851 CTGGAAGAGG TCGTCCTGGT CATTCACGAC TGGGGCTCCG CTCTGGGTTT 901 CCACTGGGCC AAGCGCAATC CAGAGCGCGT CAAAGGTATT GCATTTATGG 951 AGTTCATCCG CCCTATCCCG ACCTGGGACG AATGGCCAGA ATTTGCCCGC 1001 GAGACCTTCC AGGCCTTCCG CACCACCGAC GTCGGCCGCA AGCTGATCAT 1051 CGATCAGAAC GTTTTTATCG AGGGTACGCT GCCGATGGGT GTCGTCCGCC 1101 CGCTGACTGA AGTCGAGATG GACCATTACC GCGAGCCGTT CCTGAATCCT 1151 GTTGACCGCG AGCCACTGTG GCGCTTCCCA AACGAGCTGC CAATCGCCGG 1201 TGAGCCAGCG AACATCGTCG CGCTGGTCGA AGAATACATG GACTGGCTGC 1251 ACCAGTCCCC TGTCCCGAAG CTGCTGTTCT GGGGCACCCC AGGCGTTCTG 1301 ATCCCACCGG CCGAAGCCGC TCGCCTGGCC AAAAGCCTGC CTAACTGCAA 1351 GGCTGTGGAC ATCGGCCCGG GTCTGAATCT GCTGCAAGAA GACAACCCGG 1401 ACCTGATCGG CAGCGAGATC GCGCGCTGGC TGTCTACTCT GGAGATTTCC 1451 GGTTAATAGA ATTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT 1501 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA 1551 ATAACTAGCA TAACCCCTTG GGGCGGCCGC TTCGAGCAGA CATGATAAGA 1601 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG 1651 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA 1701 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 1751 GTTCAGGGGG AGATGTGGGA GGTTTTTTTA AGCAAGTAAA ACCTCTACAA 1801 ATGTGGTAAA ATCGAATTCT AATGGATCCT CTTTGCGCTT GCGTTTTCCC 1851 TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTCA GCACCGTTTC 1901 TGCGGACTGG CTTTCTACGT GTTCCGCTTC CTTTAGCAGC CCTTGCGCCC 1951 TGAGTGCTTG CGGCAGCGTG AGCTTCAAAA GAATTGCCAG CTGGGGCGCC 2001 CTCTGGTAAG GTTGGGAAGC CCTGCAAAGT AAACTGGATG GCTTTCTTGC 2051 CGCCAAGGAT CTGATGGCGC AGGGGATCAA GATCTGATCA AGAGACAGGA 2101 TGACGGTCGT TTCGCATGCT TGAACAAGAT GGATTGCACG CAGGTTCTCC 2151 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 2201 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 2251 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 2301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 2351 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 2401 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 2451 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 2501 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGC 2551 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 2601 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGTATGC 2651 CGGATGGTGA GGATCTCGTC GTGACTCATG GCGATGCCTG CTTGCCGAAT 2701 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 2751 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 2801 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 2851 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 2901 GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC 2951 CAACCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 3001 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 3051 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 3101 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 3151 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 3201 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 3251 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 3301 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 3351 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 3401 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 3451 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 3501 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG 3551 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 3601 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 3651 GCAGAAAAAA AGGATTTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 3701 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 3751 ATCAAAAAGG ATCTTCACCT AGATCCTTTT ATAGTCCGGA AATACAGGAA 3801 CGCACGCTGG ATGGCCCTTC GCTGGGATGG TGAAACCATG AAAAATGGCA 3851 GCTTCAGTGG ATTAAGTGGG GGTAATGTGG CCTGTACCCT CTGGTTGCAT 3901 AGGTATTCAT ACGGTTAAAA TTTATCAGGC GCGATTGCGG CAGTTTTTCG 3951 GGTGGTTTGT TGCCATTTTT ACCTGTCTGC TGCCGTGATC GCGCTGAACG 4001 CGTTTTAGCG GTGCGTACAA TTAAGGGATT ATGGTAAATC CACTTACTGT 4051 CTGCCCTCGT AGCCATCGAG ATAAACCGCA GTACTCCGGC CACGATGCGT 4101 CCGGCGTAGA GGATCGAGAT CT Vector sequence prepared 28-Jan-08.