This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLF1-HIF1a [CMV/neo] Vector sequence reference points: Base pairs 7221 CMV immediate early enhancer/promoter 1–742 Chimeric Intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 HIF1A coding region 1065–3542 Linker 3552–3563 NanoLuc® coding region 3564–4073 SV40 late poly(A) region 4210–4431 SV40 early enhancer/promoter 4530–4942 EM7 bacterial promoter 4956–5022 Neomycin phosphotransferase coding region 5036–5830 Synthetic poly(A) signal 5894–5942 ColE1-derived plasmid replication origin 6178–6214 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAG GGCGCCGGCG GCGCGAACGA CAAGAAAAAG 1101 ATAAGTTCTG AACGTCGAAA AGAAAAGTCT CGAGATGCAG CCAGATCTCG 1151 GCGAAGTAAA GAATCTGAAG TTTTTTATGA GCTTGCTCAT CAGTTGCCAC 1201 TTCCACATAA TGTGAGTTCG CATCTTGATA AGGCCTCTGT GATGAGGCTT 1251 ACCATCAGCT ATTTGCGTGT GAGGAAACTT CTGGATGCTG GTGATTTGGA 1301 TATTGAAGAT GACATGAAAG CACAGATGAA TTGCTTTTAT TTGAAAGCCT 1351 TGGATGGTTT TGTTATGGTT CTCACAGATG ATGGTGACAT GATTTACATT 1401 TCTGATAATG TGAACAAATA CATGGGATTA ACTCAGTTTG AACTAACTGG 1451 ACACAGTGTG TTTGATTTTA CTCATCCATG TGACCATGAG GAAATGAGAG 1501 AAATGCTTAC ACACAGAAAT GGCCTTGTGA AAAAGGGTAA AGAACAAAAC 1551 ACACAGCGAA GCTTTTTTCT CAGAATGAAG TGTACCCTAA CTAGCCGAGG 1601 AAGAACTATG AACATAAAGT CTGCAACATG GAAGGTATTG CACTGCACAG 1651 GCCACATTCA CGTATATGAT ACCAACAGTA ACCAACCTCA GTGTGGGTAT 1701 AAGAAACCAC CTATGACCTG CTTGGTGCTG ATTTGTGAAC CCATTCCTCA 1751 CCCATCAAAT ATTGAAATTC CTTTAGATAG CAAGACTTTC CTCAGTCGAC 1801 ACAGCCTGGA TATGAAATTT TCTTATTGTG ATGAAAGAAT TACCGAATTG 1851 ATGGGATATG AGCCAGAAGA ACTTTTAGGC CGCTCAATTT ATGAATATTA 1901 TCATGCTTTG GACTCTGATC ATCTGACCAA AACTCATCAT GATATGTTTA 1951 CTAAAGGACA AGTCACCACA GGACAGTACA GGATGCTTGC CAAAAGAGGT 2001 GGATATGTCT GGGTTGAAAC TCAAGCAACT GTCATATATA ACACCAAGAA 2051 TTCTCAACCA CAGTGCATTG TATGTGTGAA TTACGTTGTG AGTGGTATTA 2101 TTCAGCACGA CTTGATTTTC TCCCTTCAAC AAACAGAATG TGTCCTTAAA 2151 CCGGTTGAAT CTTCAGATAT GAAAATGACT CAGCTATTCA CCAAAGTTGA 2201 ATCAGAAGAT ACAAGTAGCC TCTTTGACAA ACTTAAGAAG GAACCTGATG 2251 CTTTAACTTT GCTGGCCCCA GCCGCTGGAG ACACAATCAT ATCTTTAGAT 2301 TTTGGCAGCA ACGACACAGA AACTGATGAC CAGCAACTTG AGGAAGTACC 2351 ATTATATAAT GATGTAATGC TCCCCTCACC CAACGAAAAA TTACAGAATA 2401 TAAATTTGGC AATGTCTCCA TTACCCACCG CTGAAACGCC AAAGCCACTT 2451 CGAAGTAGTG CTGACCCTGC ACTCAATCAA GAAGTTGCAT TAAAATTAGA 2501 ACCAAATCCA GAGTCACTGG AACTTTCTTT TACCATGCCC CAGATTCAGG 2551 ATCAGACACC TAGTCCTTCC GATGGAAGCA CTAGACAAAG TTCACCTGAG 2601 CCTAATAGTC CCAGTGAATA TTGTTTTTAT GTGGATAGTG ATATGGTCAA 2651 TGAATTCAAG TTGGAATTGG TAGAAAAACT TTTTGCTGAA GACACAGAAG 2701 CAAAGAACCC ATTTTCTACT CAGGACACAG ATTTAGACTT GGAGATGTTA 2751 GCTCCCTATA TCCCAATGGA TGATGACTTC CAGTTACGTT CCTTCGATCA 2801 GTTGTCACCA TTAGAAAGCA GTTCCGCAAG CCCTGAAAGC GCAAGTCCTC 2851 AAAGCACAGT TACAGTATTC CAGCAGACTC AAATACAAGA ACCTACTGCT 2901 AATGCCACCA CTACCACTGC CACCACTGAT GAATTAAAAA CAGTGACAAA 2951 AGACCGTATG GAAGACATTA AAATATTGAT TGCATCTCCA TCTCCTACCC 3001 ACATACATAA AGAAACTACT AGTGCCACAT CATCACCATA TAGAGATACT 3051 CAAAGTCGGA CAGCCTCACC AAACAGAGCA GGAAAAGGAG TCATAGAACA 3101 GACAGAAAAA TCTCATCCAA GAAGCCCTAA CGTGTTATCT GTCGCTTTGA 3151 GTCAAAGAAC TACAGTTCCT GAGGAAGAAC TAAATCCAAA GATACTAGCT 3201 TTGCAGAATG CTCAGAGAAA GCGAAAAATG GAACATGATG GTTCACTTTT 3251 TCAAGCAGTA GGAATTGGAA CATTATTACA GCAGCCAGAC GATCATGCAG 3301 CTACTACATC ACTTTCTTGG AAACGTGTAA AAGGATGCAA ATCTAGTGAA 3351 CAGAATGGAA TGGAGCAAAA GACAATTATT TTAATACCCT CTGATTTAGC 3401 ATGTAGACTG CTGGGGCAAT CAATGGATGA AAGTGGATTA CCACAGCTGA 3451 CCAGTTATGA TTGTGAAGTT AATGCTCCTA TACAAGGCAG CAGAAACCTA 3501 CTGCAGGGTG AAGAATTACT CAGAGCTTTG GATCAAGTTA ACGTTTCTCT 3551 CGGCTCGAGC GGCGTCTTCA CACTCGAAGA TTTCGTTGGG GACTGGCGAC 3601 AGACAGCCGG CTACAACCTG GACCAAGTCC TTGAACAGGG AGGTGTGTCC 3651 AGTTTGTTTC AGAATCTCGG GGTGTCCGTA ACTCCGATCC AAAGGATTGT 3701 CCTGAGCGGT GAAAATGGGC TGAAGATCGA CATCCATGTC ATCATCCCGT 3751 ATGAAGGTCT GAGCGGCGAC CAAATGGGCC AGATCGAAAA AATTTTTAAG 3801 GTGGTGTACC CTGTGGATGA TCATCACTTT AAGGTGATCC TGCACTATGG 3851 CACACTGGTA ATCGACGGGG TTACGCCGAA CATGATCGAC TATTTCGGAC 3901 GGCCGTATGA AGGCATCGCC GTGTTCGACG GCAAAAAGAT CACTGTAACA 3951 GGGACCCTGT GGAACGGCAA CAAAATTATC GACGAGCGCC TGATCAACCC 4001 CGACGGCTCC CTGCTGTTCC GAGTAACCAT CAACGGAGTG ACCGGCTGGC 4051 GGCTGTGCGA ACGCATTCTG GCGTAAGGCC GCGACTCTAG AGTCGACCTG 4101 CAGGCATGCA AGCTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG 4151 TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCGGC 4201 CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA 4251 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT 4301 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA 4351 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT 4401 TTAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGAAT TTTAACAAAA 4451 TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 4501 GCGGTATTTC ACACCGCATA CGCGGATCTG CGCAGCACCA TGGCCTGAAA 4551 TAACCTCTGA AAGAGGAACT TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC 4601 CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 4651 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 4701 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 4751 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 4801 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 4851 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 4901 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTAA 4951 TTAACTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 5001 CGACAAGGTG AGGAACTAAA CCCAGGAGGC AGATCATGAT TGAACAAGAT 5051 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 5101 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 5151 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 5201 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 5251 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 5301 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 5351 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 5401 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 5451 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 5501 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 5551 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 5601 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 5651 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 5701 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 5751 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 5801 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA 5851 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGATGGC CGCAATAAAA 5901 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA 5951 GCGATAAGGA TCCTCTTTGC GCTTGCGTTT TCCCTTGTCC AGATAGCCCA 6001 GTAGCTGACA TTCATCCGGG GTCAGCACCG TTTCTGCGGA CTGGCTTTCT 6051 ACCCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 6101 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 6151 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 6201 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 6251 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 6301 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 6351 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 6401 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 6451 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 6501 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 6551 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 6601 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 6651 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 6701 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 6751 CAGAAAAAAA GGATTTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 6801 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 6851 TCAAAAAGGA TCTTCACCTA GATCCTTTTA TAGTCCGGAA ATACAGGAAC 6901 GCACGCTGGA TGGCCCTTCG CTGGGATGGT GAAACCATGA AAAATGGCAG 6951 CTTCAGTGGA TTAAGTGGGG GTAATGTGGC CTGTACCCTC TGGTTGCATA 7001 GGTATTCATA CGGTTAAAAT TTATCAGGCG CGATTGCGGC AGTTTTTCGG 7051 GTGGTTTGTT GCCATTTTTA CCTGTCTGCT GCCGTGATCG CGCTGAACGC 7101 GTTTTAGCGG TGCGTACAAT TAAGGGATTA TGGTAAATCC ACTTACTGTC 7151 TGCCCTCGTA GCCATCGAGA TAAACCGCAG TACTCCGGCC ACGATGCGTC 7201 CGGCGTAGAG GATCGAGATC T Sequence file created 5-December-2013.