pFC17A (HaloTag(R) 7) CMVd3 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC17A (HaloTag(R) 7) CMVd3 Flexi(R) Vector sequence reference points: Base pairs 4127 CMVd3 intermediate early enhancer/promoter 1-66 T7 RNA polymerase promoter (-17 to +3) 77-96 SP6 RNA polymerase promoter (-17 to +3) 101-120 SgfI region 127-134 barnase coding region 158-493 EcoICRI region 513-518 TEV protease recognition sequence 533-553 HaloTag(R) 7 coding region 563-1453 SV40 late polyadenylation signal 1587-1808 Beta-lactamase (Ampr) coding region 2069-2929 ColE1-derived plasmid origin of replication 3084-3120 Cer region (site for E. coli XerCD recombinase) 3791-4076 .. 1 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 51 AGTGAACCGT CAGATCACTA GAAGCTTAAT ACGACTCACT ATAGGGTACG 101 ATTTAGGTGA CACTATAGAA TAAGGAGCGA TCGCCATGGA ATAAGTAAGG 151 AATCCACATG GCACAGGTTA TCAACACGTT TGACGGGGTT GCGGATTATC 201 TTCAGACATA TCATAAGCTA CCTGATAATT ACATTACAAA ATCAGAAGCA 251 CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG AACCTTGCAG ACGTCGCTCC 301 GGGGAAAAGC ATCGGCGGAG ACATCTTCTC AAACAGGGAA GGCAAACTCC 351 CGGGCAAAAG CGGACGAACA TGGCGTGAAG CGGATATTAA CTATACATCA 401 GGCTTCAGAA ATTCAGACCG GATTCTTTAC TCAAGCGACT GGCTGATTTA 451 CAAAACAACG GACCATTATC AGACCTTTAC AAAAATCAGA TAATGTTTAA 501 TGACCCCGTG TCGAGCTCTC GAGCCAACCA CTGAGGATCT GTACTTTCAG 551 AGCGATAACG ATGGATCCGA AATCGGTACT GGCTTTCCAT TCGACCCCCA 601 TTATGTGGAA GTCCTGGGCG AGCGCATGCA CTACGTCGAT GTTGGTCCGC 651 GCGATGGCAC CCCTGTGCTG TTCCTGCACG GTAACCCGAC CTCCTCCTAC 701 GTGTGGCGCA ACATCATCCC GCATGTTGCA CCGACCCATC GCTGCATTGC 751 TCCAGACCTG ATCGGTATGG GCAAATCCGA CAAACCAGAC CTGGGTTATT 801 TCTTCGACGA CCACGTCCGC TTCATGGATG CCTTCATCGA AGCCCTGGGT 851 CTGGAAGAGG TCGTCCTGGT CATTCACGAC TGGGGCTCCG CTCTGGGTTT 901 CCACTGGGCC AAGCGCAATC CAGAGCGCGT CAAAGGTATT GCATTTATGG 951 AGTTCATCCG CCCTATCCCG ACCTGGGACG AATGGCCAGA ATTTGCCCGC 1001 GAGACCTTCC AGGCCTTCCG CACCACCGAC GTCGGCCGCA AGCTGATCAT 1051 CGATCAGAAC GTTTTTATCG AGGGTACGCT GCCGATGGGT GTCGTCCGCC 1101 CGCTGACTGA AGTCGAGATG GACCATTACC GCGAGCCGTT CCTGAATCCT 1151 GTTGACCGCG AGCCACTGTG GCGCTTCCCA AACGAGCTGC CAATCGCCGG 1201 TGAGCCAGCG AACATCGTCG CGCTGGTCGA AGAATACATG GACTGGCTGC 1251 ACCAGTCCCC TGTCCCGAAG CTGCTGTTCT GGGGCACCCC AGGCGTTCTG 1301 ATCCCACCGG CCGAAGCCGC TCGCCTGGCC AAAAGCCTGC CTAACTGCAA 1351 GGCTGTGGAC ATCGGCCCGG GTCTGAATCT GCTGCAAGAA GACAACCCGG 1401 ACCTGATCGG CAGCGAGATC GCGCGCTGGC TGTCTACTCT GGAGATTTCC 1451 GGTTAATAGA ATTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT 1501 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA 1551 ATAACTAGCA TAACCCCTTG GGGCGGCCGC TTCGAGCAGA CATGATAAGA 1601 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG 1651 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA 1701 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 1751 GTTCAGGGGG AGATGTGGGA GGTTTTTTTA AGCAAGTAAA ACCTCTACAA 1801 ATGTGGTAAA ATCGAATTCT AATGGATCCT CTTTGCGCTT GCGTTTTCCC 1851 TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTCA GCACCGTTTC 1901 TGCGGACTGG CTTTCTACGT AATGGTTTCT TAGACGTCAG GTGGCACTTT 1951 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 2001 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 2051 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 2101 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 2151 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 2201 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 2251 AGAACGTTTT CCAATGATGA GCACTTTCAA AGTTCTGCTA TGTGGCGCGG 2301 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 2351 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 2401 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 2451 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACTATCGG AGGACCGAAG 2501 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 2551 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 2601 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 2651 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 2701 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 2751 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 2801 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 2851 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 2901 TAGGTGCCTC ACTGATTAAG CATTGGTAAT TCGAAATGAC CGACCAAGCG 2951 ACGCCCAACC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 3001 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 3051 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 3101 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 3151 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3201 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3251 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 3301 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 3351 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 3401 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 3451 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3501 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 3551 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 3601 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3651 TACGCGCAGA AAAAAAGGAT TTCAAGAAGA TCCTTTGATC TTTTCTACGG 3701 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 3751 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTATAGT CCGGAAATAC 3801 AGGAACGCAC GCTGGATGGC CCTTCGCTGG GATGGTGAAA CCATGAAAAA 3851 TGGCAGCTTC AGTGGATTAA GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT 3901 TGCATAGGTA TTCATACGGT TAAAATTTAT CAGGCGCGAT TGCGGCAGTT 3951 TTTCGGGTGG TTTGTTGCCA TTTTTACCTG TCTGCTGCCG TGATCGCGCT 4001 GAACGCGTTT TAGCGGTGCG TACAATTAAG GGATTATGGT AAATCCACTT 4051 ACTGTCTGCC CTCGTAGCCA TCGAGATAAA CCGCAGTACT CCGGCCACGA 4101 TGCGTCCGGC GTAGAGGATC GAGATCT Vector sequence prepared 28-Jan-08.