pHTC HaloTag(R) CMV-neo Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pHTC HaloTag(R) CMV-neo Vector sequence reference points: Base pairs 6185 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 Multiple cloning region 1052-1129 HaloTag(R) linker 1124-1158 TEV protease recognition sequence 1139-1159 HaloTag(R) coding region 1169-2059 SV40 late poly(A) region 2177-2398 SV40 enhancer and early promoter 2497-2915 SV40 minimum origin of replication 2813-2878 Neomycin phosphotransferase coding region 2960-3754 Synthetic poly(A) signal(from pCiNeo vectors) 3818-3866 Beta-lactamase coding region 4127-4987 ColE1-derived plasmid replication origin 5142-5178 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCGATCGCTT CCGAATTCCT ACCGCGGATA TCTAGATTTG 1101 GGCCCAATTC CTGCAGGCGA GCTCTCGAGC CAACCACTGA GGATCTGTAC 1151 TTTCAGAGCG ATAACGATGG ATCCGAAATC GGTACTGGCT TTCCATTCGA 1201 CCCCCATTAT GTGGAAGTCC TGGGCGAGCG CATGCACTAC GTCGATGTTG 1251 GTCCGCGCGA TGGCACCCCT GTGCTGTTCC TGCACGGTAA CCCGACCTCC 1301 TCCTACGTGT GGCGCAACAT CATCCCGCAT GTTGCACCGA CCCATCGCTG 1351 CATTGCTCCA GACCTGATCG GTATGGGCAA ATCCGACAAA CCAGACCTGG 1401 GTTATTTCTT CGACGACCAC GTCCGCTTCA TGGATGCCTT CATCGAAGCC 1451 CTGGGTCTGG AAGAGGTCGT CCTGGTCATT CACGACTGGG GCTCCGCTCT 1501 GGGTTTCCAC TGGGCCAAGC GCAATCCAGA GCGCGTCAAA GGTATTGCAT 1551 TTATGGAGTT CATCCGCCCT ATCCCGACCT GGGACGAATG GCCAGAATTT 1601 GCCCGCGAGA CCTTCCAGGC CTTCCGCACC ACCGACGTCG GCCGCAAGCT 1651 GATCATCGAT CAGAACGTTT TTATCGAGGG TACGCTGCCG ATGGGTGTCG 1701 TCCGCCCGCT GACTGAAGTC GAGATGGACC ATTACCGCGA GCCGTTCCTG 1751 AATCCTGTTG ACCGCGAGCC ACTGTGGCGC TTCCCAAACG AGCTGCCAAT 1801 CGCCGGTGAG CCAGCGAACA TCGTCGCGCT GGTCGAAGAA TACATGGACT 1851 GGCTGCACCA GTCCCCTGTC CCGAAGCTGC TGTTCTGGGG CACCCCAGGC 1901 GTTCTGATCC CACCGGCCGA AGCCGCTCGC CTGGCCAAAA GCCTGCCTAA 1951 CTGCAAGGCT GTGGACATCG GCCCGGGTCT GAATCTGCTG CAAGAAGACA 2001 ACCCGGACCT GATCGGCAGC GAGATCGCGC GCTGGCTGTC TACTCTGGAG 2051 ATTTCCGGTT AATAGAATTG GCATGCAAGC TGATCCGGCT GCTAACAAAG 2101 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA 2151 TAACCCCTTG GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG 2201 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT 2251 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA 2301 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG 2351 AGATGTGGGA GGTTTTTTTA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA 2401 ATCGAATTTT AACAAAATAT TAACGCTTAC AATTTCCTGA TGCGGTATTT 2451 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGC GGATCTGCGC 2501 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT 2551 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 2601 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 2651 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 2701 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 2751 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 2801 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 2851 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 2901 TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGGCT 2951 AGAGCCACCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC 3001 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 3051 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 3101 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC 3151 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 3201 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 3251 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC 3301 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 3351 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 3401 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG 3451 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG 3501 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 3551 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT 3601 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 3651 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 3701 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT 3751 CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT 3801 GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 3851 GTTGGTTTTT TGTGTGAATC GATAGCGATA AGGATCCTCT TTGCGCTTGC 3901 GTTTTCCCTT GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC 3951 ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGTTTCTTA GACGTCAGGT 4001 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 4051 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 4101 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 4151 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 4201 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 4251 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 4301 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTCAAAG TTCTGCTATG 4351 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 4401 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 4451 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 4501 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACTATCGGAG 4551 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 4601 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 4651 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 4701 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 4751 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 4801 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 4851 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 4901 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 4951 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAATTC GAAATGACCG 5001 ACCAAGCGAC GCCCAACCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 5051 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 5101 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 5151 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 5201 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 5251 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 5301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 5351 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 5401 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 5451 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 5501 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 5551 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 5601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 5651 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 5701 CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT 5751 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 5801 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC 5851 GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC 5901 ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC 5951 CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG 6001 CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG 6051 ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA 6101 ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC 6151 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCT Sequence file prepared 18-May-2011.