pF1A T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pF1A T7 Flexi(R) Vector sequence reference points: Base pairs 3455 T7 RNA polymerase promoter (-17 to +3) 21-40 Sgf I site 100-107 Barnase coding region 109-444 Pme I site 446-453 T7 terminator 573-620 Beta-lactamase (Ampr) coding region 954-1814 ColE1-derived plasmid origin of replication 1969-2005 cer site (site for E. coli XerCD recombinase) 2676-2961 rrnB transcription terminator 3012-3413 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG 51 CGGATAACAA TTCCCCACTA GTAATAATTT TCTTTAACTT TAGTAAGGAG 101 CGATCGCCAT GGCACAGGTT ATCAACACGT TTGACGGGGT TGCGGATTAT 151 CTTCAGACAT ATCATAAGCT ACCTGATAAT TACATTACAA AATCAGAAGC 201 ACAAGCCCTC GGCTGGGTGG CATCAAAAGG GAACCTTGCA GACGTCGCTC 251 CGGGGAAAAG CATCGGCGGA GACATCTTCT CAAACAGGGA AGGCAAACTC 301 CAGGGCAAAA GCGGACGAAC ATGGCGTGAA GCGGATATTA ACTATACATC 351 AGGCTTCAGA AATTCAGACC GGATTCTTTA CTCAAGCGAC TGGCTGATTT 401 ACAAAACAAC GGACCATTAT CAGACCTTTA CAAAAATCAG ATAATGTTTA 451 AACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC 501 ATGCAAGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC 551 TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC 601 GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGTTCGCTT 651 GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA GCCCACTGCA 701 AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC 751 AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC 801 TACGTAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 851 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 901 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 951 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 1001 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 1051 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC 1101 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 1151 GATGAGCACT TTCAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 1201 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 1251 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 1301 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 1351 CCAACTTACT TCTGACAACT ATCGGAGGAC CGAAGGAGCT AACCGCTTTT 1401 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 1451 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 1501 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 1551 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 1601 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 1651 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA 1701 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 1751 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 1801 TTAAGCATTG GTAATTCGAA ATGACCGACC AAGCGACGCC CAACCGGTAT 1851 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 1901 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 1951 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 2001 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 2051 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 2101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 2151 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 2201 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 2251 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 2301 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 2351 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 2401 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 2451 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 2501 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 2551 AGGATTTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 2601 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 2651 ATCTTCACCT AGATCCTTTT ATAGTCCGGA AATACAGGAA CGCACGCTGG 2701 ATGGCCCTTC GCTGGGATGG TGAAACCATG AAAAATGGCA GCTTCAGTGG 2751 ATTAAGTGGG GGTAATGTGG CCTGTACCCT CTGGTTGCAT AGGTATTCAT 2801 ACGGTTAAAA TTTATCAGGC GCGATTGCGG CAGTTTTTCG GGTGGTTTGT 2851 TGCCATTTTT ACCTGTCTGC TGCCGTGATC GCGCTGAACG CGTTTTAGCG 2901 GTGCGTACAA TTAAGGGATT ATGGTAAATC CACTTACTGT CTGCCCTCGT 2951 AGCCATCGAG ATAAACCGCA GTACTCCGGC CACGATGCGT CCGGCGTAGA 3001 GGATCGAGAT CTTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG 3051 GTCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT 3101 GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT 3151 GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA 3201 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT 3251 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG 3301 CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC 3351 AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT 3401 TCTACAAACT CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 3451 CTCAT Sequence file prepared 30-Nov-04.