pFC14K (HaloTag(R) 7) CMV Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC14K (HaloTag(R) 7) CMV Flexi(R) Vector sequence reference points: Base pairs 5051 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 SgfI region 1056-1063 Barnase coding region 1087-1422 EcoICRI region 1442-1447 TEV protease recognition sequence 1462-1482 HaloTag(R) 7 coding region 1492-2382 SV40 late poly(A) signal 2516-2737 Kanamycin resistance coding region 3045-3839 ColE1-derived plasmid origin of replication 4008-4044 Cer region (site for E. coli XerCD recombinase) 4715-5000 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 AGCCAACCAC TGAGGATCTG TACTTTCAGA GCGATAACGA TGGATCCGAA 1501 ATCGGTACTG GCTTTCCATT CGACCCCCAT TATGTGGAAG TCCTGGGCGA 1551 GCGCATGCAC TACGTCGATG TTGGTCCGCG CGATGGCACC CCTGTGCTGT 1601 TCCTGCACGG TAACCCGACC TCCTCCTACG TGTGGCGCAA CATCATCCCG 1651 CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA TCGGTATGGG 1701 CAAATCCGAC AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT 1751 TCATGGATGC CTTCATCGAA GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC 1801 ATTCACGACT GGGGCTCCGC TCTGGGTTTC CACTGGGCCA AGCGCAATCC 1851 AGAGCGCGTC AAAGGTATTG CATTTATGGA GTTCATCCGC CCTATCCCGA 1901 CCTGGGACGA ATGGCCAGAA TTTGCCCGCG AGACCTTCCA GGCCTTCCGC 1951 ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG TTTTTATCGA 2001 GGGTACGCTG CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG 2051 ACCATTACCG CGAGCCGTTC CTGAATCCTG TTGACCGCGA GCCACTGTGG 2101 CGCTTCCCAA ACGAGCTGCC AATCGCCGGT GAGCCAGCGA ACATCGTCGC 2151 GCTGGTCGAA GAATACATGG ACTGGCTGCA CCAGTCCCCT GTCCCGAAGC 2201 TGCTGTTCTG GGGCACCCCA GGCGTTCTGA TCCCACCGGC CGAAGCCGCT 2251 CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA TCGGCCCGGG 2301 TCTGAATCTG CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG 2351 CGCGCTGGCT GTCTACTCTG GAGATTTCCG GTTAATAGAA TTCTAGAGTC 2401 GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA 2451 GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 2501 GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA 2551 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 2601 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 2651 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG 2701 GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTCTA 2751 ATGGATCCTC TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC 2801 TGACATTCAT CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTG 2851 TTCCGCTTCC TTTAGCAGCC CTTGCGCCCT GAGTGCTTGC GGCAGCGTGA 2901 GCTTCAAAAG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC 2951 CTGCAAAGTA AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA 3001 GGGGATCAAG ATCTGATCAA GAGACAGGAT GACGGTCGTT TCGCATGCTT 3051 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 3101 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 3151 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 3201 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG 3251 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG 3301 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 3351 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 3401 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 3451 AAGCGAAACA TCGCATCGAG CGAGCACGCA CTCGGATGGA AGCCGGTCTT 3501 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA 3551 ACTGTTCGCC AGGCTCAAGG CGCGTATGCC GGATGGTGAG GATCTCGTCG 3601 TGACTCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 3651 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 3701 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 3751 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 3801 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG 3851 GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCGGTATC AGCTCACTCA 3901 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 3951 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 4001 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 4051 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 4101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 4151 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 4201 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 4251 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 4301 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 4351 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 4401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 4451 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 4501 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 4551 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATTTCAAG 4601 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 4651 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 4701 GATCCTTTTA TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG 4751 CTGGGATGGT GAAACCATGA AAAATGGCAG CTTCAGTGGA TTAAGTGGGG 4801 GTAATGTGGC CTGTACCCTC TGGTTGCATA GGTATTCATA CGGTTAAAAT 4851 TTATCAGGCG CGATTGCGGC AGTTTTTCGG GTGGTTTGTT GCCATTTTTA 4901 CCTGTCTGCT GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT 4951 TAAGGGATTA TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA 5001 TAAACCGCAG TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC 5051 T Vector sequence updated 24-Aug-07.