pFC15K (HaloTag(R) 7) CMVd1 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC15K (HaloTag(R) 7) CMVd1 Flexi(R) Vector sequence reference points: Base pairs 4177 CMVd1 intermediate early enhancer/promoter 1-121 T7 RNA polymerase promoter (-17 to +3) 132-151 SP6 RNA polymerase promoter (-17 to +3) 156-175 SgfI region 182-189 barnase coding region 213-548 EcoICRI region 568-573 TEV protease recognition sequence 588-608 HaloTag(R) 7 coding region 618-1508 SV40 late polyadenylation signal 1642-1863 Kanamycin resistance (Kanr) coding region 2171-2965 ColE1-derived plasmid origin of replication 3134-3170 Cer region (site for E. coli XerCD recombinase) 3841-4126 .. 1 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAATAACCCC GCCCCGTTGA 51 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 101 GGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTAATACGAC TCACTATAGG 151 GTACGATTTA GGTGACACTA TAGAATAAGG AGCGATCGCC ATGGAATAAG 201 TAAGGAATCC ACATGGCACA GGTTATCAAC ACGTTTGACG GGGTTGCGGA 251 TTATCTTCAG ACATATCATA AGCTACCTGA TAATTACATT ACAAAATCAG 301 AAGCACAAGC CCTCGGCTGG GTGGCATCAA AAGGGAACCT TGCAGACGTC 351 GCTCCGGGGA AAAGCATCGG CGGAGACATC TTCTCAAACA GGGAAGGCAA 401 ACTCCCGGGC AAAAGCGGAC GAACATGGCG TGAAGCGGAT ATTAACTATA 451 CATCAGGCTT CAGAAATTCA GACCGGATTC TTTACTCAAG CGACTGGCTG 501 ATTTACAAAA CAACGGACCA TTATCAGACC TTTACAAAAA TCAGATAATG 551 TTTAATGACC CCGTGTCGAG CTCTCGAGCC AACCACTGAG GATCTGTACT 601 TTCAGAGCGA TAACGATGGA TCCGAAATCG GTACTGGCTT TCCATTCGAC 651 CCCCATTATG TGGAAGTCCT GGGCGAGCGC ATGCACTACG TCGATGTTGG 701 TCCGCGCGAT GGCACCCCTG TGCTGTTCCT GCACGGTAAC CCGACCTCCT 751 CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC CCATCGCTGC 801 ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG 851 TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC 901 TGGGTCTGGA AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG 951 GGTTTCCACT GGGCCAAGCG CAATCCAGAG CGCGTCAAAG GTATTGCATT 1001 TATGGAGTTC ATCCGCCCTA TCCCGACCTG GGACGAATGG CCAGAATTTG 1051 CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG CCGCAAGCTG 1101 ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT 1151 CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA 1201 ATCCTGTTGA CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC 1251 GCCGGTGAGC CAGCGAACAT CGTCGCGCTG GTCGAAGAAT ACATGGACTG 1301 GCTGCACCAG TCCCCTGTCC CGAAGCTGCT GTTCTGGGGC ACCCCAGGCG 1351 TTCTGATCCC ACCGGCCGAA GCCGCTCGCC TGGCCAAAAG CCTGCCTAAC 1401 TGCAAGGCTG TGGACATCGG CCCGGGTCTG AATCTGCTGC AAGAAGACAA 1451 CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCT ACTCTGGAGA 1501 TTTCCGGTTA ATAGAATTCT AGAGTCGACC TGCAGGCATG CAAGCTGATC 1551 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT 1601 GAGCAATAAC TAGCATAACC CCTTGGGGCG GCCGCTTCGA GCAGACATGA 1651 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA 1701 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 1751 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT 1801 TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTTAAGCAA GTAAAACCTC 1851 TACAAATGTG GTAAAATCGA ATTCTAATGG ATCCTCTTTG CGCTTGCGTT 1901 TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC 1951 GTTTCTGCGG ACTGGCTTTC TACGTGTTCC GCTTCCTTTA GCAGCCCTTG 2001 CGCCCTGAGT GCTTGCGGCA GCGTGAGCTT CAAAAGAATT GCCAGCTGGG 2051 GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT 2101 CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT GATCAAGAGA 2151 CAGGATGACG GTCGTTTCGC ATGCTTGAAC AAGATGGATT GCACGCAGGT 2201 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 2251 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 2301 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 2351 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 2401 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT 2451 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 2501 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 2551 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 2601 CACGCACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 2651 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 2701 TATGCCGGAT GGTGAGGATC TCGTCGTGAC TCATGGCGAT GCCTGCTTGC 2751 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 2801 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA 2851 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 2901 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 2951 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 3001 ACGCCCAACC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 3051 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 3101 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 3151 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 3201 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3251 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3301 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 3351 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 3401 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 3451 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 3501 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3551 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 3601 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 3651 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3701 TACGCGCAGA AAAAAAGGAT TTCAAGAAGA TCCTTTGATC TTTTCTACGG 3751 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 3801 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTATAGT CCGGAAATAC 3851 AGGAACGCAC GCTGGATGGC CCTTCGCTGG GATGGTGAAA CCATGAAAAA 3901 TGGCAGCTTC AGTGGATTAA GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT 3951 TGCATAGGTA TTCATACGGT TAAAATTTAT CAGGCGCGAT TGCGGCAGTT 4001 TTTCGGGTGG TTTGTTGCCA TTTTTACCTG TCTGCTGCCG TGATCGCGCT 4051 GAACGCGTTT TAGCGGTGCG TACAATTAAG GGATTATGGT AAATCCACTT 4101 ACTGTCTGCC CTCGTAGCCA TCGAGATAAA CCGCAGTACT CCGGCCACGA 4151 TGCGTCCGGC GTAGAGGATC GAGATCT Vector sequence prepared 28-Jan-08.