pFN21A (HaloTag(R) 7) CMV Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN21A (HaloTag(R) 7) CMV Flexi(R) Vector sequence reference points: Base pairs 5064 CMV intermediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 HaloTag(R) 7 protein coding region 1067-1957 TEV region 1970-1990 SgfI region 1997-2004 Barnase coding region 2028-2363 Pme I region 2365-2372 SV40 late polyadenylation signal 2524-2745 Beta-lactamase (Ampr) coding region 3006-3866 ColE1-derived plasmid origin of replication 4021-4057 cer region (for E. coli XerCD recombinase) 4728-5013 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC 1101 CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT 1151 CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC 1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA 1251 TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT 1301 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT 1351 GGGTCTGGAA GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG 1401 GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT 1451 ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC 1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA 1551 TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC 1601 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA 1651 TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG 1701 CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG 1751 CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT 1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT 1851 GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC 1901 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT 1951 TTCCGGCGAG CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACGCGA 2001 TCGCCATGGA ATAAGTAAGG AATCCACATG GCACAGGTTA TCAACACGTT 2051 TGACGGGGTT GCGGATTATC TTCAGACATA TCATAAGCTA CCTGATAATT 2101 ACATTACAAA ATCAGAAGCA CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG 2151 AACCTTGCAG ACGTCGCTCC GGGGAAAAGC ATCGGCGGAG ACATCTTCTC 2201 AAACAGGGAA GGCAAACTCC CGGGCAAAAG CGGACGAACA TGGCGTGAAG 2251 CGGATATTAA CTATACATCA GGCTTCAGAA ATTCAGACCG GATTCTTTAC 2301 TCAAGCGACT GGCTGATTTA CAAAACAACG GACCATTATC AGACCTTTAC 2351 AAAAATCAGA TAATGTTTAA ACGAATTCGG GCTCGGTACC CGGGGATCCT 2401 CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA TCCGGCTGCT AACAAAGCCC 2451 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA 2501 CCCCTTGGGG CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT 2551 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 2601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA 2651 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA 2701 TGTGGGAGGT TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 2751 GAATTCTAAT GGATCCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC 2801 CCAGTAGCTG ACATTCATCC GGGGTCAGCA CCGTTTCTGC GGACTGGCTT 2851 TCTACGTAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 2901 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 2951 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 3001 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG 3051 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 3101 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 3151 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 3201 ATGATGAGCA CTTTCAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 3251 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 3301 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG 3351 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 3401 GGCCAACTTA CTTCTGACAA CTATCGGAGG ACCGAAGGAG CTAACCGCTT 3451 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 3501 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 3551 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 3601 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA 3651 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 3701 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 3751 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 3801 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 3851 GATTAAGCAT TGGTAATTCG AAATGACCGA CCAAGCGACG CCCAACCGGT 3901 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 3951 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 4001 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 4051 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 4101 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 4151 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 4201 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 4251 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 4301 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 4351 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 4401 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 4451 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 4501 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 4551 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 4601 AAAGGATTTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 4651 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 4701 GGATCTTCAC CTAGATCCTT TTATAGTCCG GAAATACAGG AACGCACGCT 4751 GGATGGCCCT TCGCTGGGAT GGTGAAACCA TGAAAAATGG CAGCTTCAGT 4801 GGATTAAGTG GGGGTAATGT GGCCTGTACC CTCTGGTTGC ATAGGTATTC 4851 ATACGGTTAA AATTTATCAG GCGCGATTGC GGCAGTTTTT CGGGTGGTTT 4901 GTTGCCATTT TTACCTGTCT GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG 4951 CGGTGCGTAC AATTAAGGGA TTATGGTAAA TCCACTTACT GTCTGCCCTC 5001 GTAGCCATCG AGATAAACCG CAGTACTCCG GCCACGATGC GTCCGGCGTA 5051 GAGGATCGAG ATCT File updated 28-Apr-08.