pFN23A (HaloTag(R)) CMVd2 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN23A (HaloTag(R)) CMVd2 Flexi(R) Vector sequence reference points: Base pairs 4140 CMVd2 intermediate early enhancer/promoter 1-73 T7 RNA polymerase promoter (-17 to +3) 84-103 SP6 RNA polymerase promoter (-17 to +3 108-127 HaloTag(R) 7 protein coding region 143-1033 TEV region 1046-1066 SgfI region 1073-1080 Barnase coding region 1104-1439 Pme I region 1441-1448 SV40 late polyadenylation signal 1600-1821 Beta-lactamase (Ampr) coding region 2082-2942 ColE1-derived plasmid origin of replication 3097-3133 cer region (for E. coli XerCD recombinase) 3804-4089 .. 1 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 51 CTGGTTTAGT GAACCGTCAG ATCACTAGAA GCTTAATACG ACTCACTATA 101 GGGTACGATT TAGGTGACAC TATAGAATAA GGAGAATTCA TCATGGCAGA 151 AATCGGTACT GGCTTTCCAT TCGACCCCCA TTATGTGGAA GTCCTGGGCG 201 AGCGCATGCA CTACGTCGAT GTTGGTCCGC GCGATGGCAC CCCTGTGCTG 251 TTCCTGCACG GTAACCCGAC CTCCTCCTAC GTGTGGCGCA ACATCATCCC 301 GCATGTTGCA CCGACCCATC GCTGCATTGC TCCAGACCTG ATCGGTATGG 351 GCAAATCCGA CAAACCAGAC CTGGGTTATT TCTTCGACGA CCACGTCCGC 401 TTCATGGATG CCTTCATCGA AGCCCTGGGT CTGGAAGAGG TCGTCCTGGT 451 CATTCACGAC TGGGGCTCCG CTCTGGGTTT CCACTGGGCC AAGCGCAATC 501 CAGAGCGCGT CAAAGGTATT GCATTTATGG AGTTCATCCG CCCTATCCCG 551 ACCTGGGACG AATGGCCAGA ATTTGCCCGC GAGACCTTCC AGGCCTTCCG 601 CACCACCGAC GTCGGCCGCA AGCTGATCAT CGATCAGAAC GTTTTTATCG 651 AGGGTACGCT GCCGATGGGT GTCGTCCGCC CGCTGACTGA AGTCGAGATG 701 GACCATTACC GCGAGCCGTT CCTGAATCCT GTTGACCGCG AGCCACTGTG 751 GCGCTTCCCA AACGAGCTGC CAATCGCCGG TGAGCCAGCG AACATCGTCG 801 CGCTGGTCGA AGAATACATG GACTGGCTGC ACCAGTCCCC TGTCCCGAAG 851 CTGCTGTTCT GGGGCACCCC AGGCGTTCTG ATCCCACCGG CCGAAGCCGC 901 TCGCCTGGCC AAAAGCCTGC CTAACTGCAA GGCTGTGGAC ATCGGCCCGG 951 GTCTGAATCT GCTGCAAGAA GACAACCCGG ACCTGATCGG CAGCGAGATC 1001 GCGCGCTGGC TGTCGACGCT CGAGATTTCC GGCGAGCCAA CCACTGAGGA 1051 TCTGTACTTT CAGAGCGATA ACGCGATCGC CATGGAATAA GTAAGGAATC 1101 CACATGGCAC AGGTTATCAA CACGTTTGAC GGGGTTGCGG ATTATCTTCA 1151 GACATATCAT AAGCTACCTG ATAATTACAT TACAAAATCA GAAGCACAAG 1201 CCCTCGGCTG GGTGGCATCA AAAGGGAACC TTGCAGACGT CGCTCCGGGG 1251 AAAAGCATCG GCGGAGACAT CTTCTCAAAC AGGGAAGGCA AACTCCCGGG 1301 CAAAAGCGGA CGAACATGGC GTGAAGCGGA TATTAACTAT ACATCAGGCT 1351 TCAGAAATTC AGACCGGATT CTTTACTCAA GCGACTGGCT GATTTACAAA 1401 ACAACGGACC ATTATCAGAC CTTTACAAAA ATCAGATAAT GTTTAAACGA 1451 ATTCGGGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA 1501 AGCTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG 1551 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCGGC CGCTTCGAGC 1601 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC 1651 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT 1701 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA 1751 TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TTAAGCAAGT 1801 AAAACCTCTA CAAATGTGGT AAAATCGAAT TCTAATGGAT CCTCTTTGCG 1851 CTTGCGTTTT CCCTTGTCCA GATAGCCCAG TAGCTGACAT TCATCCGGGG 1901 TCAGCACCGT TTCTGCGGAC TGGCTTTCTA CGTAATGGTT TCTTAGACGT 1951 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 2001 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 2051 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2101 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 2151 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 2201 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 2251 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT CAAAGTTCTG 2301 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 2351 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2401 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 2451 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACTAT 2501 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 2551 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 2601 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 2651 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 2701 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 2751 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 2801 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 2851 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 2901 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AATTCGAAAT 2951 GACCGACCAA GCGACGCCCA ACCGGTATCA GCTCACTCAA AGGCGGTAAT 3001 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 3051 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 3101 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 3151 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 3201 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 3251 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 3301 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 3351 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 3401 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 3451 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 3501 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT 3551 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 3601 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 3651 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATTTCAAGA AGATCCTTTG 3701 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 3751 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAT 3801 AGTCCGGAAA TACAGGAACG CACGCTGGAT GGCCCTTCGC TGGGATGGTG 3851 AAACCATGAA AAATGGCAGC TTCAGTGGAT TAAGTGGGGG TAATGTGGCC 3901 TGTACCCTCT GGTTGCATAG GTATTCATAC GGTTAAAATT TATCAGGCGC 3951 GATTGCGGCA GTTTTTCGGG TGGTTTGTTG CCATTTTTAC CTGTCTGCTG 4001 CCGTGATCGC GCTGAACGCG TTTTAGCGGT GCGTACAATT AAGGGATTAT 4051 GGTAAATCCA CTTACTGTCT GCCCTCGTAG CCATCGAGAT AAACCGCAGT 4101 ACTCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT File updated 28-Apr-08.