pFN24A (HaloTag(R)) CMVd3 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN24A (HaloTag(R)) CMVd3 Flexi(R) Vector sequence reference points: Base pairs 4133 CMVd3 intermediate early enhancer/promoter 1-66 T7 RNA polymerase promoter (-17 to +3) 77-96 SP6 RNA polymerase promoter (-17 to +3 101-120 HaloTag(R) 7 protein coding region 136-1026 TEV region 1039-1059 SgfI region 1066-1073 Barnase coding region 1097-1432 PmeI region 1434-1441 SV40 late polyadenylation signal 1593-1814 Beta-lactamase (Ampr) coding region 2075-2935 ColE1-derived plasmid origin of replication 3090-3126 cer region (for E. coli XerCD recombinase) 3797-4082 .. 1 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 51 AGTGAACCGT CAGATCACTA GAAGCTTAAT ACGACTCACT ATAGGGTACG 101 ATTTAGGTGA CACTATAGAA TAAGGAGAAT TCATCATGGC AGAAATCGGT 151 ACTGGCTTTC CATTCGACCC CCATTATGTG GAAGTCCTGG GCGAGCGCAT 201 GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG CTGTTCCTGC 251 ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT 301 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC 351 CGACAAACCA GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG 401 ATGCCTTCAT CGAAGCCCTG GGTCTGGAAG AGGTCGTCCT GGTCATTCAC 451 GACTGGGGCT CCGCTCTGGG TTTCCACTGG GCCAAGCGCA ATCCAGAGCG 501 CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC CCGACCTGGG 551 ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC 601 GACGTCGGCC GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC 651 GCTGCCGATG GGTGTCGTCC GCCCGCTGAC TGAAGTCGAG ATGGACCATT 701 ACCGCGAGCC GTTCCTGAAT CCTGTTGACC GCGAGCCACT GTGGCGCTTC 751 CCAAACGAGC TGCCAATCGC CGGTGAGCCA GCGAACATCG TCGCGCTGGT 801 CGAAGAATAC ATGGACTGGC TGCACCAGTC CCCTGTCCCG AAGCTGCTGT 851 TCTGGGGCAC CCCAGGCGTT CTGATCCCAC CGGCCGAAGC CGCTCGCCTG 901 GCCAAAAGCC TGCCTAACTG CAAGGCTGTG GACATCGGCC CGGGTCTGAA 951 TCTGCTGCAA GAAGACAACC CGGACCTGAT CGGCAGCGAG ATCGCGCGCT 1001 GGCTGTCGAC GCTCGAGATT TCCGGCGAGC CAACCACTGA GGATCTGTAC 1051 TTTCAGAGCG ATAACGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG 1101 CACAGGTTAT CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT 1151 CATAAGCTAC CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG 1201 CTGGGTGGCA TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA 1251 TCGGCGGAGA CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC 1301 GGACGAACAT GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA 1351 TTCAGACCGG ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG 1401 ACCATTATCA GACCTTTACA AAAATCAGAT AATGTTTAAA CGAATTCGGG 1451 CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT 1501 CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC 1551 TGAGCAATAA CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG 1601 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 1651 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1701 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 1751 TTTCAGGTTC AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT 1801 CTACAAATGT GGTAAAATCG AATTCTAATG GATCCTCTTT GCGCTTGCGT 1851 TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC 1901 CGTTTCTGCG GACTGGCTTT CTACGTAATG GTTTCTTAGA CGTCAGGTGG 1951 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 2001 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 2051 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC 2101 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 2151 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 2201 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 2251 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTCAAAGTT CTGCTATGTG 2301 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 2351 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA 2401 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 2451 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC TATCGGAGGA 2501 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 2551 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 2601 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 2651 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT 2701 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2751 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 2801 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 2851 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 2901 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAATTCGA AATGACCGAC 2951 CAAGCGACGC CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 3001 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 3051 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 3101 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 3151 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 3201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 3251 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 3301 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 3351 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 3401 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT 3451 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 3501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 3551 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 3601 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 3651 GCAGATTACG CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT 3701 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 3751 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG 3801 AAATACAGGA ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT 3851 GAAAAATGGC AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC 3901 TCTGGTTGCA TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG 3951 GCAGTTTTTC GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT 4001 CGCGCTGAAC GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT 4051 CCACTTACTG TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG 4101 CCACGATGCG TCCGGCGTAG AGGATCGAGA TCT File updated 28-Apr-08.