Overview
Flexi® Vector Primer Design Tool
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The Flexi® Vector Systems use two rare-cutting restriction enzymes, Sgf I and Pme I, in a simple, yet powerful, directional cloning method for protein-coding sequences. These systems provide a rapid, efficient and high-fidelity way to transfer these sequences between a variety of Flexi Vectors. The Flexi Vectors contain various expression or peptide tag options to enable expression of native or fusion proteins to study protein structure and function, as well as protein-protein interactions.
This software will design PCR primers for use with Flexi® Vectors which will amplify a coding region from your input sequence. These primers will have the following characteristics
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