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Plate-Based Assay Methods for the Assessment of Cellular Health

Andrew L. Niles is a Senior Research Scientist in the Assay Designs Group at Promega Corporation (Madison, WI). His laboratory is focused on developing homogeneous “add-mix-measure” assay chemistries for cell-based studies. The lab utilizes fluorescent and/or bioluminescent detection platforms configured in plate-based, multiplexed formats to detect cytotoxicity and elucidate mechanism of action. These efforts have led to multiple commercialized assay systems in the area of in vitro toxicity testing. He has been awarded eight U.S. Patents relating to cell and protease biology, authored several book chapters and reviews, and is an active member of Society for Laboratory Automation, Laboratory Robotics Interest Group, and American Association for Cancer Research.

  • Andrew L. Niles

  • Original Webinar Date: Tuesday, April 10, 2012 Tuesday, April 10, 2012

ABSTRACT

Cell-based assay methods continue to be an important aspect of experimental biology. Therefore, the assessment of cell health after experimental treatment or manipulation is a necessary activity. We have developed a suite of chemistries which can be conveniently applied to plated cells to define their relative health and viability. This seminar uses several examples to detail how dosage and intrinsic cellular susceptibility shape the kinetics of a cytotoxic response. Particular regard will be given to multiplexed formats which can be used to define mechanistic cytotoxicity. Practical considerations and tips for successful implementation will also be discussed.

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