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Massively Parallel Sequencing for Forensic DNA Analysis

Doug Storts is responsible for strategic development of Promega reagent systems for nucleic acid amplification, expression analysis, and genotyping (human forensic, paternity, clinical research, cell authentication).

Doug has co-authored 30 peer-reviewed manuscripts and 55 articles and chapters in non-refereed journals and books. He has presented numerous lectures and posters at national and international meetings, and is a co-inventor on several issued and pending patents. Doug has served as an invited lecturer for the University of Wisconsin - Madison, an ad hoc reviewer for several scientific journals and has participated on committees establishing national and international scientific policy.

Doug received his Bachelor of Science degree in Biology at Manchester College, and his Master of Science degree and Ph.D. in Microbiology at Miami University. Prior to joining Promega in 1991, he was a post-doctoral research associate and instructor in the Department of Biochemistry and Molecular Biology at the University of Chicago.

  • Douglas R. Storts, Ph.D.

  • Head of Research, Nucleic Acid Technologies, Promega Corporation

  • Original Webinar Date: Tuesday, December 8, 2015

In this webinar, we presented data demonstrating performance of the PowerSeq™ Systems, and outline the strengths and limitations of massively parallel sequencing technologies for routine forensic analysis. The prototype PowerSeq™ Systems include primers and amplification master mix for sequencing autosomal short tandem repeats (STRs), Y-chromosome STRs, the mitochondrial DNA control region, and various combinations of the three on an Illumina MiSeq® System.

Information

The prototype PowerSeq™ Systems include primers and amplification master mix for sequencing autosomal short tandem repeats (STRs), Y-chromosome STRs, the mitochondrial DNA control region, and various combinations of the three on an Illumina MiSeq® System. The selected STR loci are the same as used in the commercial PowerPlex® Fusion and PowerPlex® Y23 Systems that are routinely used for capillary electrophoresis-based forensic DNA analysis. In this webinar, we will present data demonstrating performance of the PowerSeq™ Systems, and outlining the strengths and limitations of current massively parallel sequencing technologies for routine forensic analysis.