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Making Ultrasensitive Endogenous NanoLuc® Luciferase Reporter Cell Lines Using CRISPR

Eric Rhodes has been the Chief Technology Officer at Horizon Discovery since early 2012. Eric spent ten years with Sangamo BioSciences as VP Business Development & Strategic Alliance followed by three years at Sigma-Aldrich as Director of Business Development where he was deeply involved in applications of ZFN technology.
  • Eric Rhodes, Horizon Discovery Group, UK

  • Sr. Vice President R&D and Chief Technical Officer

  • Original Webinar Date: Tuesday, May 20, 2014

Gene editing techniques like CRISPR hold great promise in moving from over expression systems to actual monitoring of endogeneous transcript levels.  This webinar will discuss the utility of a small, extremely bright luciferase as a means of monitoring endogeneous transcript levels as well as discussing relevant points to consider when building such model systems. View full abstract below.

 

This webinar will be approximately 1 hour in length.

Abstract

Cell lines that utilize fusions of endogenous genes to a highly sensitive reporter like NanoLuc® Luciferase offer researchers the ability to easily screen and monitor cellular responses in their natural genomic context with a reporter expressed at biologically relevant levels. CRISPR holds great promise for generating these endogenous gene fusions, but there are still a number of important considerations to take into account when undertaking such an experiment. We will discuss the relevant points to consider in building a NanoLuc® Luciferase reporter line and demonstrate how the high-intensity luminescence of NanoLuc® Luciferase together with CRISPR gene editing technique can enable the generation of endogenous reporter cell lines.
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