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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

How to Avoid Artificial Non-Enzymatic PTMs During the Peptide Sample Preparation Process

Proteomics

Tuesday, June 13, 2017

Sergei Saveliev, PhD

Non-enzymatic post-translational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. These modifications negatively affect efficacy and stability of biotherapeutic proteins. Major non-enzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These non-enzymatic PTMs are also introduced during protein preparation for peptide mapping and compromise the analysis. Join us as we will discuss sources of these artificial protein modifications as well as procedural optimizations to suppress these PTMs.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Y-Screening and Direct Amplification of Sexual Assault Evidence Kit Samples

Genetic Identity

Monday, October 31, 2016

Ms. Amy McGuckian
Technical Leader
Forensic Biology Unit
Palm Beach County Sheriff’s Office


Dr. Anupama Gopalakrishnan
Product Manager
Genetic Identity
Promega Corporation

Screening for sperm in sexual assault samples is a laborious process with limited sensitivity compared to male DNA detection by amplification. Direct amplification of casework samples reduces the potential for DNA loss during purification, a special concern with low-template samples. In this webinar, Promega presents an overview of the Casework Direct Kit, and the Palm Beach County Sheriff’s Office describes how they plan to use the kit in their sexual assault evidence processing.

Faster Mass Spec: Same-Day Sample Prep Now a Reality

Proteomics

Tuesday, October 25, 2016

Michael M. Rosenblatt, PhD

Traditional protocols require digestion times of up to 18 hours, as well as reduction and alkylation prior to digestion. These denaturants often inhibit enzymes and modify proteins, and require removal prior to analysis. What if you could shorten digestion times to ~30 minutes, without denaturants or reductants or alkylating agents?

Immune Checkpoint Bioassays Power Combination Immunotherapy

Drug Discovery

Tuesday, September 27, 2016

Richard Somberg, PhD

Therapeutic antibodies designed to block or activate immune checkpoint receptors are a powerful new approach to the treatment of cancer and other diseases. However, due to their biologic complexity the development of therapeutic antibodies requires unique functional and analytical tools. Here we present a suite of MOA-based functional bioassays for use in antibody screening, characterization, potency testing and stability studies.

GTPase Biology: New Tools to the Rescue

Cell-Based Assays

Tuesday, August 23, 2016

Subhanjan Mondal, PhD

Cell Signaling is key to controlling cell health, and one key signaling node is GTPases. Technical options to analyze the biochemical activities of this incredibly important class of proteins are very limited. To support the needs of the research community, we will present various novel ways to analyze GTPase functions.

Introduction to Real-Time PCR: Basic Principles and Chemistries

Genomics

Tuesday, August 09, 2016

Leta Steffen, PhD

Real-Time PCR (qPCR) is the workhorse of most gene expression analysis. This webinar will provide an overview of basic qPCR principles compared to traditional PCR and common terms used in assay design and analysis.

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