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Home » Resources »

Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

Biologics Forum: Tips and Tools for Successful Immunotherapy Bioassay Development

Drug Discovery

Tuesday, April 23, 2019

Zhijie Jey Cheng, PhD, Manuela Grassi, PhD and Richard Somberg, PhD

Mechanism of action (MOA)-based bioassays are a critical component of biologics drug development. However, establishing a functional bioassay that meets the regulatory guidelines for use in potency determination and lot release can be challenging. Join our panel of bioassay experts for a live discussion on bioassay design and selection, assay optimization, and bioassay qualification for potency lot release.

The Y-HRD Database: How It Works and How to Use It in Casework

Genetic Identity

Wednesday, May 08, 2019

Lutz Roewer, PhD

The Y-Chromosome Haplotype Reference Database (Y-HRD) includes Y-chromosome haplotype data from 135 national databases populated over the past 20 years. Y-based ancestry prediction and familial searching can provide important investigative leads. This webinar will discuss how to use the Y-HRD to facilitate statistical analyses for a range of casework applications and interpretation purposes (e.g., frequency estimation, kinship and mixture analysis and ancestry prediction).

Developing More Efficient Biologic Workflows with Automated Plasmid Preps and Protein/Antibody Purification

Genomics

Tuesday, May 14, 2019

Dr. Nidhi Nath, Doug White and Chris D'Jamoos

Development and screening of biologics can be a time-consuming process due to the volume of samples processed for cloning, isolation of plasmids and purification of candidate antibodies. This webinar will discuss how our field-based automation team can help you develop walk-away automation solutions to speed up your purification workflow.

Studying Endogeneous Protein Dynamics with CRISPR-Mediated Tagging: Understanding Your Options

Cell-Based Assays

Tuesday, June 04, 2019

Christopher Eggers, PhD

An exciting application of CRISPR/Cas9-mediated gene editing is the ability to tag and measure endogenously expressed proteins while maintaining native expression levels and regulation. In this webinar, we will discuss available options for simple and efficient knock-in of a bioluminescent peptide tag to enable sensitive, homogeneous assays for measuring protein dynamics.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

Genomics

Tuesday, February 14, 2017

Doug Wieczorek, PhD

Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.