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Molecular Diagnostics (MDx)
Tuesday, January 23, 2018
Matthew Robers, MS
Although a number of extracellular techniques exist to quantitatively measure kinase-ligand binding or inhibition, until now there was no means by which to quantitate target occupancy or drug affinity in live cells. In this webinar we report the use of an energy transfer technique (NanoBRET) in the first quantitative approach to profiling kinase occupancy of a test compound inside live cells.
Friday, December 08, 2017
Dr. Ashley Anderson
While no case is without its challenges, medical conditions such as chimerism and genetic outliers (e.g., identical twins) add wrinkles to the analysis and interpretation of the DNA profiles obtained in the case. Medical procedures such as bone marrow transplants can also confuse the results, affecting the legal, social and ethical deliberations of the case. In this webinar, Dr. Anderson will review the interpretation and reporting difficulties experienced when medical conditions have the potential to influence forensic casework results.
Tuesday, December 05, 2017
Christopher Mason, PhD
Many new methods in genomics enable an integrative, cross-kingdom view of patients (precision metagenomics) and their environment, including metagenome profiles of the world’s cities (MetaSUB.org) and antimicrobial resistance (AMR) markers. In this webinar, technologies that can sequence, quantify, and map nucleic acids will be covered, for Earth and beyond, focusing on methods from Promega that enable these projects and missions.
Tuesday, November 14, 2017
Terry Riss, PhD
With traditional cell-based endpoint assays you can miss important cellular events if measurement isn't taken at the appropriate time, particularly when characterizing a compound's effect on cells. Additionally, assay reagents can negatively affect cell health and endpoint assays can impede downstream multiplexing.
Tuesday, October 24, 2017
Marie Schwinn, PhD
CRISPR/Cas9 gene editing has emerged as a powerful tool for making precise genomic modifications. Although often used to create gene knockouts, the technology also enables targeted knock-in of a specific sequence. An exciting application is the endogenous tagging of proteins expressed under native regulatory conditions. In this webinar, we will detail a simple and efficient cloning-free method for CRISPR-mediated endogenous gene tagging, focusing on knock-in of the sensitive, bioluminescent HiBiT tag.