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CloneWeaver® Workflow Purchasing Tool

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Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. Every cloning project is unique. Read More

Once you have designed the cloning scheme, gathering all of the required reagents to get you from construct to expression and analysis is not trivial. CloneWeaver helps you build a customized cloning "kit" with all of the items your cloning scheme requires. Purchase your selection instantly, save it or email it to a purchasing agent. Soon you'll have everything you require to create the construct you need.

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Vectors: Bacterial Change

Name Description Part Number
PinPoint Xa Protein Purification System Open/Close Add
In vivo biotin tags make purification easy; many proteins produced are soluble
Purify fusion proteins by column or batch methods
PinPoint™ Vectors are supplied for all reading frames

The PinPoint Xa Protein Purification System is designed for the production and purification of fusion proteins that are biotinylated in vivo. The DNA coding for the protein of interest is cloned into a PinPoint Vector downstream of a sequence encoding a peptide that becomes biotinylated in vivo. Biotinylated fusion proteins are produced in E. coli and are affinity-purified using the SoftLink Soft Release Avidin Resin. This proprietary resin allows elution of the fusion protein under nondenaturing conditions. The PinPoint Vectors feature the encoded endoproteinase Factor Xa (pronounced ten a) proteolytic site that provides a way to separate the purification tag from the native protein, and the vectors carry a convenient multiple cloning region for ease in construction of fusion proteins. The system contains vectors in all possible sense reading frames, an avidin-conjugated resin, Streptavidin-Alkaline Phosphatase, a purification column and biotin. The PinPoint Xa Control Vector contains the chloramphenicol acetyltransferase (CAT) gene and is provided as a means of monitoring protein expression, purification and processing conditions. The system generally yields 1-5mg of protein per liter of culture.

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pGEM-9Zf(-) Vector Open/Close Add
Insert can be excised from vector with the SP6 and T7 RNA polymerase promoters
Blue/white screening to identify recombinant clones
Multiple cloning site provides a convenient selection of restriction sites for cloning

The pGEM-9Zf(-) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, efficient synthesis of RNA in vitro and the production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.

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pGEM-7Zf(+/-) Vectors Open/Close Add
Blue/white screening for easily identifying recombinant clones
In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
Orientation of the f1 origin differentiates vectors (+ and –)

The pGEM-7Zf(+) and pGEM-7Zf(-) Vectors are derivatives of the pGEM-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System. pGEM-7Zf(+) and pGEM-7Zf(-) Vectors are identical except for the orientation of the f1 origin.

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pGEM-5Zf(+) Vector Open/Close Add
Blue/white screening to easily identify recombinant clones
Multiple cloning site provides a convenient selection of restriction sites for cloning

The pGEM-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.

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pGEM-11Zf(+) Vector Open/Close Add
Blue/white screening for easy identification of recombinant clones
In vitro transcription from SP6 and T7 RNA polymerase promoters that flank the multiple cloning region
Multiple cloning region provides a convenient selection of restriction sites for cloning

The pGEM-11Zf(+) and pGEM-11Zf(-) Vectors can be used as standard cloning vectors, as templates for in vitro transcription and for the production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII. The pGEM-11Zf(-) and pGEM-11Zf(+) Vectors are identical except for the orientation of the f1 origin.

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