Insert can be excised from vector with the SP6 and T7 RNA polymerase promoters
Blue/white screening to identify recombinant clones
Multiple cloning site provides a convenient selection of restriction sites for cloning
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The pGEM-9Zf(-) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, efficient synthesis of RNA in vitro and the production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.
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Blue/white screening for easily identifying recombinant clones
In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
Orientation of the f1 origin differentiates vectors (+ and –)
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The pGEM-7Zf(+) and pGEM-7Zf(-) Vectors are derivatives of the pGEM-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System. pGEM-7Zf(+) and pGEM-7Zf(-) Vectors are identical except for the orientation of the f1 origin.
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Blue/white screening to easily identify recombinant clones
Multiple cloning site provides a convenient selection of restriction sites for cloning
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The pGEM-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.
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Blue/white screening for easy identification of recombinant clones
In vitro transcription from SP6 and T7 RNA polymerase promoters that flank the multiple cloning region
Multiple cloning region provides a convenient selection of restriction sites for cloning
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The pGEM-11Zf(+) and pGEM-11Zf(-) Vectors can be used as standard cloning vectors, as templates for in vitro transcription and for the production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII. The pGEM-11Zf(-) and pGEM-11Zf(+) Vectors are identical except for the orientation of the f1 origin.
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