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CloneWeaver® Workflow Purchasing Tool

Powering Your Cloning Workflow

Product selection has never been easier

Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. Every cloning project is unique. Read More

Once you have designed the cloning scheme, gathering all of the required reagents to get you from construct to expression and analysis is not trivial. CloneWeaver helps you build a customized cloning "kit" with all of the items your cloning scheme requires. Purchase your selection instantly, save it or email it to a purchasing agent. Soon you'll have everything you require to create the construct you need.

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Restriction Enzymes

Name Incubation Conditions Description Part Number
XhoI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

C/TCGA G G AGCT\C

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XbaI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

T/CTAG A A GATC\T

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TaqI Open/Close Add

T/CG A A GC\T

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SphI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G CATG/C C\GTAC G

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SpeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

A/CTAG T T GATC\A

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SmaI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

CCC/GGG GGG\CCC

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SgfI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

GCG AT/CGC CGC\TA GCG

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ScaI Open/Close Add
Capable of digesting DNA in 15 minutes or less

AGT/ACT TCA\TGA

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SalI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

G/TCGA C C AGCT\G

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SacII Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

CC GC/GG GG\CG CC

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SacI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G AGCT/C C\TCGA G

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RsaI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Available at high concentration, containing 5,000 units of RsaI at a concentration of 40–80u/μl

GT/AC CA\TG

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PvuI Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix

CG AT/CG GC\TA GC

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PstI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications

C TGCA/G G\ACGT C

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NotI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications
High concentration product no longer available

GC/GGCC GC CG CCGG\CG

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NheI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Genome qualified to ensure optimal performance in genomic analysis applications

G/CTAG C C GATC\G

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NdeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix

CA/TA TG GT AT\AC

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NcoI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

C/CATG G G GTAC\C

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MULTI-CORE Buffer Pack Open/Close Add
Enables digestion of DNA with more than one enzyme in a single reaction
Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions

The MULTI-CORE Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE Buffer.

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MspI Open/Close Add

C/CG G G GC\C

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MluI Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

A/CGCG T T GCGC\A

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MboI Open/Close Add

/GATC CTAG\

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KpnI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G GTAC/C C\CATG G

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I-PpoI (Intron-Encoded Endonuclease) Open/Close Add
Genome qualified to ensure optimal performance in genomic analysis applications

CTCTC TTAA/GGTAGC GAGAG\AATT CCATCG

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HpaII Open/Close Add

C/CG G G GC\C

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HinfI Open/Close Add

G/ANT C C TNA\G

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HindIII Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

A/AGCT T T TCGA\A

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HhaI Open/Close Add

G CG/C C\GC G

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HaeIII Open/Close Add
Capable of digesting DNA in 15 minutes or less

GG/CC CC\GG

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EcoRV Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

GAT/ATC CTA\TAG

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EcoRI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl

G/AATT C C TTAA\G

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DpnI Open/Close Add
Capable of digesting DNA in 15 minutes or less

G(me)A/TC CT\(me)AG

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DdeI Open/Close Add
Capable of digesting DNA in 15 minutes or less

C/TNA G G ANT\C

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ClaI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

AT/CG AT TA GC\TA

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CfoI Open/Close Add

G CG/C C\GC G

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BglII Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix
The 10,000u size is no longer available

A/GATC T T CTAG\A

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BglI Open/Close Add
Genome qualified to ensure optimal performance in genomic analysis applications
The 5,000u size is no longer available

GCCN NNN/NGGC CGGN\NNN NCCG

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BclI Open/Close Add
Genome qualified to ensure optimal performance in genomic analysis applications

T/GATC A A CTAG\T

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BamHI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Available at high concentration, containing 12,500 units of BamHI at a concentration of 40–80u/μl

G/GATC C C CTAG\G

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ApaI Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G GGCC/C C\CCGG G

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AluI Open/Close Add
Capable of digesting DNA in 15 minutes or less

AG/CT TC\GA

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AgeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix

A/CCGG T T GGCC\A

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4-CORE Buffer Pack Open/Close Add
Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers

The 4-CORE Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers.

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