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Int. J. Legal Med. 115, 128-134. Analysis of disputed single-parent/child and sibling relationships using 16 STR loci. 2001

Thomson, J.A., Ayres, K.L., Pilotti, V., Barrett, M.N., Walker, J.I. and Debenham, P.G.

Notes: In this study the PowerPlex® 1.2 System and the FFFL Multiplex (F13A01, FESFPS, F13B, LPL) were used in a validation study of short tandem repeat systems for parentage testing. (2274)

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Nat. Biotechnol. 19, 677-679. Bidirectionalization of polar promoters in plants. 2001

Xie, M., He, Y. and Gan, S.

Notes: In this paper, extracted total RNA was treated with RQ1 RNase-Free DNase prior to RT-PCR analysis. (2365)

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J. Clin. Microbiol. 39, 3512-3519. Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan. 2001

Yu, L., Wang, Z., Jiang, Y., Chang, L. and Kwang, J.

Notes: The RNAgents® Total RNA Isolation System was used to isolate viral RNA from the pellet resulting from centrifugation of 20ml of allantoic fluid or tissue culture supernatants. The isolated RNA was used for RT-PCR. (2575)

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J. Forensic Sci. 46(3), 637-641. Concordance study on population database samples using the PowerPlex™ 16 kit and AmpFLSTR® Profiler Plus™ kit and AmpFLSTR® COfiler™ kit. 2001

Budowle, B., and Sprecher, C.J.

Notes: The PowerPlex® 16 System was used in a concordance study involving more than 500 population database samples that included African Americans, Bahamians, and Southwestern Hispanics. Samples were typed using PowerPlex® 16 and the Profiler Plus™ and COfiler™ kits. The results show that the primers used in these systems are reliable for typing reference samples destined for use in CODIS. (2253)

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J. Neurosci. 21, 934–943. Differing, spatially restricted roles of ionotropic glutamate receptors in regulating the migration of GnRH neurons during embryogenesis. 2001

Simonian, S.X. and Herbison, A.E.

Notes: The SV Total RNA Isolation System was used to isolated RNA from various dissected mouse brain areas. Ten microliters of the isolated RNA were used for RT-PCR analysis of gene expression patterns. (2186)

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Proc. Natl. Acad. Sci. USA 98, 1182–1187. From the Cover: Polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease (ADPKD), is a Ca2+-permeable nonselective cation channel 2001

Gonzalez-Perrett, S., Kim, K., Ibarra, C., Damiano, A.E., Zotta, E., Batelli, M., Harris, P.C., Reisin, I.L., Arnaout, M.A. and Cantiello, H.F.

Notes: The SV Total RNA Isolation System was used to isolated RNA from the syncytiotrophoblast of human term placentas. The isolated RNA was used for RT-PCR with M-MLV Reverse Transcriptase used for the RT reaction. The 110 kDa PKD2 protein was expressed in vitro with the TNT® T7 Coupled Reticulocyte Lysate System with and without Canine Pancreatic Microsomal Membranes. The channel activity of the expressed protein was examined and the functional channel could be inhibited by known inhibitors. Expressing the luciferase control with the membranes did not produce the same effect. (2187)

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Oncogene 20, 1041-1051. Isolation and characterization of the TERE1 gene, a gene down-regulated in transitional cell carcinoma of the bladder 2001

McGarvey, T.W., Nguyen, T., Tomaszewski, J.E., Monson, F.C. and Malkowicz, S.B.

Notes: The cDNA for the TERE1 gene was amplified by PCR and directly subcloned into the pTARGET™ Mammalian Expression Vector. The 338-amino acid (36.8kDa) TERE1 protein was stably expressed in three human bladder transitional cell carcinomas (J82, T24 and 1376 TCC) and the human embryonic kidney cell line, HEK 293. Stable transfectants were selected with the antibiotic G-418. Overexpression of the protein caused 80-90% inhibition of cellular proliferation in two of the transitional cell carcinoma cell lines and inhibition of aneuploidy in the third. Transfections were accomplished in 24-well plates with the Tfx™-20 Transfection Reagent. Excellent details of the 1 hour transfection protocol are presented. (2596)

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Physiol. Genomics 5, 187–192. Microarray analysis of nicotine-induced changes in gene expression in endothelial cells. 2001

Zhang, S., Day, I.N.M. and Ye, S.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from primary human coronary artery endothelial cells. The isolated RNA was used to make gene array 33P-labeled targets on nylon cDNA microarray filters using Oligo(dT) and M-MLV Reverse Transcriptase, RNase H-. The same material was used for first-strand synthesis in RT-PCR. (2697)

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Nucl. Acids Res. 29, 4502-4508. Mitochondrial DNA deletion mutations are concomitant with ragged red regions of individual, aged muscle fibers: analysis by laser-capture microdissection. 2001

Cao, Z., Wanagat, J., McKiernan, S.H. and Aiken J.M.

Notes: Taq Polymerase was used to amplify mitochondrial sequences from various laser-capture microdissected rat DNA samples. A 15.7 kilobase PCR fragment was cloned into the pGEM®-T Easy Vector. The resultant clone was used to identify sequence breakpoints. (3234)

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Oncogene 20, 260-269. Modulation of apoptosis by procaspase-2 short isoform: selective inhibition of chromatin condensation, apoptotic body formation and phosphatidylserine externalization 2001

Droin, N., Rebe, C., Bichat, F., Hammann, A., Bertrand, R., and Solary, E.

Notes: The cDNA for the short isoform of the caspase-2 mRNA was amplified and cloned directly into the pTARGET™ Mammalian Expression Vector. The protein was stably expressed in U937 human leukemic cells by selection with the antibiotic G-418.  Stable clones were identified by PCR of the genomic DNA of selected colonies with primers to the T7 promoter of the pTARGET™ Vector and the downstream primer of the caspase-2 message. Overexpression of the protein interfered with etoposide-mediated apoptosis. (2597)

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Am. J. Pathol. 158, 373–379. Molecular analysis of mutant and wild-type Tau deposited in the brain affected by the FTDP-17 R406W mutation. 2001

Miyasaka, T., Morishima-Kawashima, M., Ravid, R., Heutink, P., van Swieten, J.C., Nagashima, K. and Ihara, Y.

Notes: The SV Total RNA Isolation System was used to isolated RNA from autopsy samples of human brain with FTDP-17 disorder. The isolated RNA was used for semi-quantitative RT-PCR (2162)

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Int. J. Legal Med. 115, 57-60. Multiplex PCR amplification of eight STR loci in Austrian and Croatian Caucasian populations. 2001

Ross, J., Parson, W., Furac, I., Kubat, M. and Holland, M.

Notes: This study reports Austrian and Croatian population data for the loci contained in the PowerPlex® 1.2 System. (2276)

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J. Exp. Biol. 204, 2005-2012. Na++K+ -ATPase in gills of the blue crab Callinectes sapidus: cDNA sequencing and salinity-related expression of the α-subunit mRNA and protein. 2001

Towle, D.W., Paulsen, R.S., Weihrauch, D., Kordylewski, M., Salvador, C., Lignot, J.-H., and Spanings-Pierrot, C.

Notes: Total RNA was isolated from Blue crab gills with the RNAgents® Total RNA Isolation System. The isolated RNA was used for RT-PCR. (2562)

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Proc. Natl. Acad. Sci. USA 98, 14738-14743. Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase oxygenase (RubisCO) supports photosynthesis and growth in tobacco. 2001

Whitney, S.M. and Andrews, J.T.

Notes: These researchers created alkaline phosphatase-conjugated probes that were specific for the α-proteobacterium Rhodospirillum rubrum RubisCO gene, rbcL. These probes were used in Southern and Northern blot analysis of non-transformed and transformed tobacco rubrum plants using the AttoPhos® AP Fluorescent Substrate System, a Vistra Fluorimager™ and ImageQuant™ software. The AttoPhos® Substrate was also used to visualize immunoblots of various proteins in transformed and non-transformed plants. To confirm their results, the researchers PCR-amplified choroplast DNA and ligated the products into the pGEM®-T Easy Vector for BigDye® Sequencing. (2649)

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Genome Res. 11(10), 1758-1765. Protein-protein interaction panel using mouse full-length cDNAs. 2001

Suzuki, H., Fukunishi, Y., Kagawa, I., Saito, R., Oda, H., Endo, T., Kondo, S., Bono, H., Okazaki, Y., Hayashizaki, Y.

Notes: The authors of this study have developed a high throughput method for screening protein-protein interactions in 384-well plates. Using elements of the CheckMate™ Mammalian Two-Hybrid System, mouse cDNAs were linked to the GAL4 DNA binding and VP16 transcription activation domains via a linear cassette PCR strategy. The linear DNAs were mixed with the pG5luc plasmid, transfection reagent and CHO-K1 cells.  Twenty hours after transfection, activation of luciferase by GAL4 binding was assayed using the Steady-Glo® Luciferase Assay System. Biotec and Tecan liquid handlers were used to semiautomate the system, allowing screening of 20,000 assay wells per day. (2727)

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J. Bacteriol. 183, 7094-7101. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions. 2001

Vandecasteele, S.J., Peetermans, W.E., Merckx, R. and Van Eldere, J.

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

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Appl. Environ. Microbiol. 67, 3295-3298. Rapid approach to determine rrn arrangement in Salmonella serovars. 2001

Helm, R.A. and Maloy, S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from Salmonella enterica serovars.  The isolated DNA was used as template in PCR reactions with 49 primer sets to examine the structure of the rrn operon. Some amplimers were up to 7kb in size. (2297)

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Nucl. Acids Res. 29, e73-e79. Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method). 2001

He, M. and Taussig, M.J.

Notes: The authors used TNT® T7 Quick for PCR DNA to produce protein arrays. Proteins were generated by in vitro translation directly from PCR fragments. (2138)

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Forensic Sci. Int. 119(3), 328-329. STR data for the GenePrint® PowerPlex® 1.2 System loci from three United Arab Emirates populations. 2001

Mohammed, A.A., Linacre, A.M., Vanezis, P. and Goodwin, W.

Notes: This is a population study reporting allele frequencies for the loci contained in the GenePrint® PowerPlex® 1.2 System. (2278)

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Forensic Sci. Int. 124(1), 47-54. STR primer concordance study. 2001

Budowle, B., Masibay, A., Anderson, S.J., Barna, C., Biega, L., Brenneke, S., Brown, B.L., Cramer, J., DeGroot ,G.A., Douglas, D., Duceman, B., Eastman, A., Giles, R., Hamill, J., Haase, D.J., Janssen, D.W., Kupferschmid, T.D., Lawton, T., Lemire, C., Llewellyn, B., Moretti, T., Neves, J., Palaski, C., Schueler, S., Sgueglia, J., Sprecher, C., Tomsey, C. and Yet, D.

Notes: This study reports that the primers used in the PowerPlex® 16 System are reliable for typing reference samples that are used in the FBI CODIS database. The authors also report allele frequency data for the Penta D and Penta E loci. (2275)

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EMBO J. 20, 3229-3237. Telomere resolution in the Lyme disease spirochete. 2001

Chaconas, G., Stewart, P.E., Tilly, K., Bono, J.L., and Rosa, P.

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from 5ml early stationary phase cultures of Borrelia burgdorferi.  Isolated genomic DNA was used in both Southern blotting and PCR. (2294)

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Development 128, 4705-4714. The Drosophila daughterless gene autoregulates and is controlled by both positive and negative cisregulation. 2001

Smith III, J.E. and Cronmiller, C.

Notes: RQ1 RNase-Free DNase was used to treat RNA samples purified from Drosophila. The DNase-treated RNA samples were then used in real-time RT-PCR to analyze gal4 and Mdh1 reporter construct transcripts driven by in vivo daughterless factor autoregulation during heat shock. (3025)

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J. Bacteriol. 183, 6801–6806. Transcriptional Regulation of furA and katG upon Oxidative Stress in Mycobacterium smegmatis. 2001

Milano, A., Forti, F., Sala, C., Riccardi, G. and Ghisotti, D.

Notes: Total RNA was isolated from Mycobacterium smegmatis for Northern blot analysis and RT-PCR. Transcripts for furA and katG were amplified by RT-PCR using Promega's MMLV Reverse Transcriptase and cloned into the pGEM®-T Easy vector. Northern blot probes for furA and katG were synthesized by in vitro transcription from the pGEM®-T Easy vector. (2310)

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J. Forensic Sci. 46(1), 178. Uruguayan population data for eight STR loci. 2001

Pagano, S., Alvarez, C., Entrala, C., Lorente, J.A., Lorente, M., Budowle, B. and Villanueva, E.

Notes: The PowerPlex® 1.2 system was used in this population study. (2268)

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J. Forensic Sci. 46(3), 647-660. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. 2001

Moretti, T.R., Baumstark, A.L., Defenbaugh, D.A., Keys, K.M., Smerick, J.B. and Budowle B.

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex® Systems were evaluated, along with other commercially available STR typing systems. (2267)

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