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Proc. Natl. Acad. Sci. USA 94, 4211-4216. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6- desaturated fatty acids in transgenic tobacco. 1997

Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R., Napier, J.A.

Notes: The authors use the Reverse Transcription System, Wizard® PCR Preps DNA Purification Systems, Wizard® Plus Minipreps DNA Purification System and the pGEM®-T Vector System in their studies. (0445)

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J. Biol. Chem. 272, 5936-5942. Nerve growth factor up-regulates the N-methyl-D-aspartate receptor subunit 1 promoter in PC12 cells. 1997

Bai, G. and Kusiak, J.W.

Notes: Reporter vectors (produced with the pGL2 Basic Vector) were transiently transfected into PC12 cells and promoter activity was measured in response to 2.5S NGF. Promoter constructs were generated by PCR, subcloned into the pGEM®-T Vector and sequenced. The pGEM®-luc Vector was used to generated an antisense luc RNA probe used in RNase protection assays (using RNase ONE™ Ribonuclease) to follow the transcription of luciferase DNA following NGF treatment. The early growth reaction transcription factor and variants were expressed in the TNT® SP6 Coupled Reticulocyte Lysate System and used to perform gel shifts with promoter elements. The SP1 Consensus Oligonucleotide was used to compete transcription factor binding to the promoter elements. (1493)

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J. Neurosci. 17, 765-773. Consequences of nigrostriatal denervation of the functioning of the basal ganglia in human and nonhuman primates: An in situ hybridization study of cytochrome oxidase subunit I mRNA. 1997

Vila, M., Levy, R., Herrero, M.T., Ruberg, M., Faucheux, B., Obeso, J.A., Agid, Y. and Hirsch, E.C.

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM®-T Vector. RNA probes were produced from the pGEM®-T Vector for in situ hybridization studies. (1547)

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J. Biol. Chem. 272, 27218-27223. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. 1997

Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

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J. Neurosci. 17, 9145-9156. Estradiol Enhances Prostaglandin E2 Receptor Gene Expression in Luteinizing Hormone-Releasing Hormone (LHRH) Neurons and Facilitates the LHRH Response to PGE2 by Activating a Glia-to-Neuron Signaling Pathway 1997

Rage, F., Lee, B.J., Ma, Y. J., Ojeda, S. R.

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM®-T Vector System. (0529)

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J. Biochem. (Tokyo) 272, 1842-1848. Molecular Cloning, Characterization, and Regulation of the Human Mitochondrial Serine Hydroxymethyltransferase Gene. 1997

Stover, P., Chen, L., Suh, J., Stover, D., Keyomarsi, K., Shane, B.

Notes: Tth DNA Polymerase was used to perform RT-PCR with a two-buffer protocol. (1936)

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Proc. Natl. Acad. Sci. USA 94, 6216-6221. A new member of the tumor necrosis factor/nerve growth factor receptor family inhibits T cell receptor–induced apoptosis. 1997

Nocentini, G., Giunchi, L., Ronchetti, S., Krausz, L.T., Bartoli, A., Moraca, R., Migliorati, G. and Riccardi, C.

Notes: The TNT® Coupled Transcription/Translation System was used to verify the 25.3kDa size of the cloned receptor. AMV reverse transcriptase was used for first strand cDNA synthesis prior to PCR. (1520)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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Proc. Natl. Acad. Sci. USA 93, 13036-13041. Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish. 1996

Zardoya, R. , Abouheif, E. , Meyer, A.

Notes: PCR products were cloned into the pGEM®-T Vector System. (0082)

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Proc. Natl. Acad. Sci. USA 93, 14972-14977. Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco. 1996

Yang, Y. and Klessig, D.F.

Notes: The pGEM®-T Vector System was used to clone PCR products amplified from a tobacco cDNA library. (1968)

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J. Clin. Invest. 98(1), 43-49. Acute regulation by insulin of phosphatidylinositol-3-kinase, Rad, Glut 4, and lipoprotein lipase mRNA levels in human muscle. 1996

Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.

Notes: Promega's Tth DNA Polymerase, pGEM®-T Vector System and Riboprobe® System were used in this study. (2002)

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J. Mol. Neurosci. 7, 193-201. Four repeat high-mol-wt MAP2 forms in rat dorsal root ganglia. 1996

Forleo, P., Couchie, D., Chabas, S. and Nunez, J.

Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)

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J. Clin. Invest. 98(2), 405-417. Cloning of the mammalian Type II Iodothyronine Deiodinase: A selenoprotein differentially expressed and regulated in human and rat brain and other tissues. 1996

Croteau, W., Davey, J.C., Galton, V.A. and St. Germain, D.L.

Notes: Promega's Access RT-PCR System was used in this study. (1997)

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J. Biol. Chem. 270, 21021-21027. Hypoxic regulation of lactate dehydrogenase A. Interaction between hypoxia-inducible factor 1 and cAMP response elements. 1995

Firth, J. D. , Ebert, B. L. , Ratcliffe, P. J.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to produce 14 consecutive 4bp deletions in a putative response element. (1157)

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