Notes: The TNT^® Coupled Transcription/Translation System was used to verify the 25.3kDa size of the cloned receptor. AMV reverse transcriptase was used for first strand cDNA synthesis prior to PCR. (1520)

Notes: Promega's Access RT-PCR system, Taq DNA Polymerase and PolyATtract^® mRNA Isolation System were used in this study. (1991)

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard^® PCR Preps DNA Purification System and cloned with the aid of the pGEM^®-T Vector System. (0624)

Notes: RT-PCR was performed with degenerate primers and the resulting 110bp product was subcloned with the pGEM®-T Vector System. The 110bp fragment was used to screen a cDNA library and four positive plaques were identified. (0650)

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

Notes: The RNasin^® Ribonuclease Inhibitor and the pGEM^®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM^®-T Vector. RNA probes were produced from the pGEM^®-T Vector for in situ hybridization studies. (1547)

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM^®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM^®-T Vector and sequenced. (1419)

Notes: Promega's Saccharomyces cerevisiae genomic DNA was used as the template for PCR amplification of several genes. (1161)

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM^®-T Vector System. (0529)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: The pGEM^®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

Notes: PolyATtract^® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM^®-T Vector. (0748)

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM^®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM^®-T Vector System. (0998)

Notes: PolyA⁺ RNA was isolated from rat total RNA using the PolyATtract^® mRNA Isolation System and used for Northern analysis. The pGEM^®-T Vector System was used to clone products from RT-PCR. (0447)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)

Notes: The pGEM^®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM^®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

Notes: Promega's Tth DNA Polymerase, pGEM^®-T Vector System and Riboprobe^® System were used in this study. (2002)

Notes: Promega's Access RT-PCR System was used in this study. (1997)

Notes: PCR products were cloned into the pGEM^®-T Vector System. (0082)

Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)