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Vet. Parasitol. 133, 283-287. Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing. 2005

Itagaki, T., Kinoshita, S., Aoki, M., Itoh, M., Saeki, H., Sato, N., Uetsuki, J., Izumiyama, S., Yagita, K., and Endo, T.

Notes: GoTaq® DNA Polymerase was used in PCR genotyping of Giardia intestinalis. Primers were designed around a 177 bp sequence of the glutamete dehydrogenase gene (gdh). Typing was based on previously reported assemblages of gdh from cats, dogs, cows and monkeys. PCR was performed in a total reaction volume of 25μl using 1.25 units of GoTaq® DNA Polymerase.

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Croat. Med. J. 46, 530–539. Twelve-year experience in identification of skeletal remains from mass graves. 2005

Andelinovic, S., Sutlovic, D., Erceg Ivkosic, I., Skaro, V., Ivkosic, A., Paic, F., Rezic, B., Definis-Gojanovic, M. and Primorac D.

Notes: These authors used DNA typing to identify human skeletal remains found in mass graves. DNA was isolated using standard phenol/chloroform extraction, the DNA IQ™ System or other methods. A modified DNA IQ™ System protocol was developed using 2g of pulverized bone. DNA was quantitated using the AluQuant® Human DNA Quantitation System or Quanti-Blot™ Human DNA quantitation kit. DNA typing was performed using several STR amplification kits, including the PowerPlex® 16 System. In some cases mitochondrial DNA testing was necessary due to the degree of nuclear DNA degradation. Of the 481 samples, 385 were amplified successfully and 109 sets of remains were identified. (3640)

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J. Forensic Sci. 49, 754–759. A simple and efficient method for extracting DNA from old and burned bone. 2004

Ye, J., Ji, A., Parra, E.J., Zheng, X., Jiang, C., Zhao, X., Hu, L. and Tu, Z.

Notes: These authors used a new DNA purification method that combines cetyltrimethylammonium bromide (CTAB) and isoamyl alcohol/chloroform extraction to isolate DNA from bones soaked in water, burned bones, or bones that had been buried for a long time. Following the CTAB and isoamyl alcohol/chloroform extraction, PCR inhibitors were removed using the DNA IQ™ System or the QIAquick PCR Purification Kit. The authors preferred the DNA IQ™ System because of its speed and ease-of-use. (3642)

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DNA IQ™ System

J. Forensic Sci. 49, 29-39. Application of the BioMek 2000 Laboratory Automation Workstation and the DNA IQ System to the extraction of forensic casework samples. 2004

Greenspoon, S.A., Ban, J.D., Sykes, K., Ballard, E.J., Edler, S.S., Baisden, M., and Covington, B.L.

Notes: This paper discusses automated DNA purification from a variety of forensic casework samples using the DNA IQ™ System on a Beckman BioMek® 2000 Laboratory Automated Workstation.  DNA was purified from various forensic casework samples including vaginal swabs, blood stains, tissue and samples.  The researchers also tested diluted and contaminated samples and the ability of the DNA IQ™ System on the Beckman BioMek® 2000 to purify DNA from these samples.  The PowerPlex® 1.1 System was used as a representative forensic laboratory typing system to test the contaminant level in all of the DNA IQ™ purified DNA samples.  (3052)

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Minerva Ginecol. 56(3), 189-96. Intracytoplasmic sperm injection. Accomplishments and qualms. 2004

Neri, Q.V., Tanaka, N., Wang, A., Katagiri, Y., Takeuchi, T., Rosenwaks, Z. and Palermo, G.D.

Notes: These authors assessed the risk of transmitting genetic defects to children after intracytoplasmic sperm injection (ICSI) for treatment of male factor infertility. Genomic DNA was isolated from blood of both parents and children using the Wizard® Genomic DNA Purification Kit. Multiplex amplification of 22 sequence tagged sites on the Y chromosome was then performed on 123 samples using the Y Chromosome Deletion Detection System, Version 1.1 and a prototype Multiplex E from Promega. (3117)

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J. Bacteriol. 186, 3214-3223. Salmonella enterica Serovar Typhi strains from which SPI7, a 134-kilobase island with genes for Vi exopolysaccharide and other functions, has been deleted. 2004

Nair, S., Alokam, S., Kothapalli, S., Porwollik, S., Proctor, E., Choy, C., McClelland, M., Liu, S.L. and Sanderson, K.E.

Notes: In this study, the Wizard® Genomic DNA Purification Kit was used to isolate chromosomal DNA from Salmonella enterica serovar Typhi. The isolated DNA was used in multiplex PCR to determine the presence or absence of certain DNA regions in various clinical isolates.  (3127)

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Biochimie 86, 849-856. Targeted disruption of the perxisomal thiolase B gene in mouse: A new model to study disorders related to peroxisomal lipid metabolism. 2004

Chevillard, G., Clémencet, M.C., Latruffe, N., and Nicolas-Francès, V.

Notes: GoTaq® DNA Polymerase was used in a multiplexed genotyping reaction with three primers to differentiate wild-type, heterozygous, and mutant transgenic mouse alleles simultaneously. The wild-type target was 670bp and the mutant or knockout target was 1,030bp. Various other amplimers (1.4kb, 471bp, & 311bp) were subcloned with the aid of the pGEM®-T Easy Vector System. (3360)

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Int. Congr. Ser. 1239, 609–11. Evaluation of Powerplex™ 16 for typing of degraded DNA samples. 2003

Glock, B., Reisacher, R.B.K.. Rennhofer, S.O., Tröscher, D., Dauber, E.M. and Mayr, W.R.

Notes: The authors evaluated the ability of the PowerPlex® 16 System to amplify degraded DNA samples and compared the results with those obtained using the AmpFlSTR® SGM Plus® kit. They amplified DNA from six whole blood samples that had been stored at 4°C or room temperature for 20 days, DNA isolated from six pairs of archived whole blood samples, and from corresponding plasma samples that had been stored below –20°C for 1–3 years and were assumed to be degraded. Amplifications were assembled and performed using protocols optimized in their laboratory. For the whole blood and plasma samples stored below –20°C, the PowerPlex® 16 System gave eight full profiles and the SGM Plus® kit gave six full profiles. No differences were observed between the two kits with DNA extracts stored at 4°C or room temperature. As expected, the frequency of allele dropout increased as the amplified fragment length increased. (3876)

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J. Clin. Microbiol. 41, 3064–3069. Invasive disease due to nontypeable Haemophilus influenzae among children in Arkansas. 2003

O’Neill, J.M., St. Geme III, J.W., Cutter, D., Adderson, E.E., Anyanwu, J., Jacobs, R.F. and Schutze, G.E.

Notes: The Wizard® Genomic DNA Purification System was used to isolate genomic DNA from clinically isolated Haemophilus influenzae samples.  The genomic DNA was then digested with EcoRI and used in Southern blots to identify the presence of various Haemophilus influenzae adhesin genes. (3102)

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Nat. Biotechnol. 21(10), 1233-1237. Large-scale genotyping of complex DNA. 2003

Kennedy, G.C., Matsuzaki, H., Dong, S., Liu, W.M., Huang, J., Liu, G., Su, X., Cao, M., Chen, W., Zhang, J., Liu, W., Yang, G., Di, X., Ryder, T., He, Z., Surti, U., Phillips, M.S., Boyce-Jacino, M.T., Fodor, S.P. and Jones, K.W.

Notes: Terminal Deoxynucleotidyl Transferase was used to biotin end-label PCR products generated from adaptor-ligated genomic DNA fragment templates. The labeled probes were then hybridized to microarrays spotted with SNP alleles. The Terminal Deoxynucleotidyl Transferase reaction was performed for 4 hours at 37°C using 15-20 units TdT, TdT reaction buffer and 18μM biotin-ddATP. (2758)

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Nucl. Acids Res. 31 (7), e35. Light-directed 5´-->3´ synthesis of complex oligonucleotide microarrays. 2003

Albert, T.J., Norton, J., Ott, M., Richmond, T., Nuwaysir, K., Nuwaysir, E.F., Stengele, K.P. and Green, R.D.

Notes: The PolyATtract® System 1000 was used to isolate mRNA from mouse spleen. The researchers used 3μg of the isolated poly(A)+ RNA in reverse transcriptase reactions to make cDNAs. The cDNAs were ultimately used as templates in in vitro transcription reactions to produce biotin labeled cRNAs that were used as targets in microarray analysis experiments.  (2743)

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J. Clin. Microbiol. 41, 4537-4541. Madurella mycetomatis Strains from Mycetoma Lesions in Sudanese Patients Are Clonal. 2003

Ahmed, A., van de Sande, W., Verbrugh, H., Fahal, A., and van Belkum, A.

Notes: Genomic DNA was isolated from Madurella mycetomatis samples.  A modified  Wizard® Genomic DNA Purification Kit protocol was used for the isolations.  Mycelia were sonicated and ground in liquid nitrogen before the addition of Nuclei Lysis Solution. The purified DNA was used in PCR.  (3049)

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Int. Congr. Ser. 1239, 125–30. PowerPlex™ 16 analysis in the Japanese population. 2003

Hashiyada, M., Itakura, Y. and Nata, M.

Notes: The authors generated population data from 508 unrelated Japanese individuals using the PowerPlex® 16 System. DNA was collected as buccal swabs and isolated using Chelex® resin followed by phenol:chloroform extraction and ethanol precipitation. For each PowerPlex® 16 reaction, 2ng of DNA was amplified using a GeneAmp® PCR System 9700, and amplified products were detected using the ABI PRISM® 377 DNA Sequencer. (3843)

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J. Forensic Sci. 47, 773-785. Validation of a 16-locus fluorescent multiplex system. 2003

Krenke, B.E., Tereba, A., Anderson, S.J., Buel, E., Culhane, S., Finis, C.J., Tomsey, C.S., Zachetti, J.M., Masibay, A., Rabbach, D.R., Amiott, E.A. and Sprecher, C.J.

Notes: In this paper, researchers from both Promega and state crime laboratories provide an evaluation of the Powerplex® 16 System loci (CODIS, Penta D, Penta E, and amelogenin). Data from several laboratories using a variety of thermal cyclers and the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer are presented. The Powerplex® 16 System was able to consistently genotype samples from as little as 0.0625-2ng of genomic DNA template. Other factors such as reaction volume, annealing temperature, titration of AmpliTaq Gold® DNA Polymerase, magnesium, or primer pairs, and 1ng DNA mixtures were analyzed using the Powerplex® 16 System.  The researchers also present data on stutter analysis and cross reactivity of primer sets on primate DNA templates for each loci. (2675)

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J. Forensic Sci. 46(3), 736-761. Allele frequencies for fourteen STR loci of the PowerPlex™ 1.1. and 2.1 multiplex systems and Penta D locus in caucasians, african-americans, hispanics, and other populations of the United States of America and Brazil. 2001

Levedakou, E.N., Freeman, D.A., Budzynski, M.J., Early, B.E., McElfresh, K.C., Schumm, J.W., Amin, A.S., Kim, Y.K., Sprecher, C.J., Krenke, B.E., Silva, D.A., McIntosh, T.M., Grubb, J.C., Johnston, L.J., Sailus, J.S., Ban, J.D., Crouse, C.A. and Nelson, M.S.

Notes: This paper details allele frequencies for the loci contained in the PowerPlex™ 1.1 and 2.1 Systems. (2262)

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Mol. Diag. 6, 55-61. Analysis of the factor V Leiden mutation using the READIT Assay. 2001

Rhodes, R.B., Lewis, K., Shultz, J., Huber, S., Voelkerding, K.V., Leonard, D.G., Tsongalis, G.J. and Kephart, D.D.

Notes: A variety of methods exist for the detection of single-nucleotide polymorphisms (SNPs) present in amplified segments of genomic DNA. The authors show the application of Promega's READIT® SNP Genotyping System, a novel SNP scoring tool, for analysis of the factor V Leiden mutation. (2139)

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Croat. Med. J. 42(3), 260-266. DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples. 2001

Alonso, A., Andelinovic, S., Martin, P., Sutlovic, D., Erceg, I., Huffine, E., de Simon, L.F., Albarran, C., Definis-Gojanovic, M., Fernandez-Rodriguez, A., Garcia, P., Drmic, I., Rezic, B., Kuret, S., Sancho, M. and Primorac, D.

Notes: The authors report use of the PowerPlex® 16 System for STR analysis of DNA from skeletal remains. (2279)

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J. Forensic Sci. 46(1), 111-115. DNA typing results from two urban subpopulations of Pakistan. 2001

Rahman, Z., Afroze, T. and Weir, B.S.

Notes: The GenePrint® CSF1PO, TPOX, TH01 Multiplex (CTT) was used in this population study. With this system amplified products are detected by silver staining following denaturing polyacrylamide gel electrophoresis. (2269)

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J. Forensic Sci. 46(3), 647-660. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. 2001

Moretti, T.R., Baumstark, A.L., Defenbaugh, D.A., Keys, K.M., Smerick, J.B. and Budowle B.

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex® Systems were evaluated, along with other commercially available STR typing systems. (2267)

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J. Forensic Sci. 45(4), 886-890. A novel approach to obtaining reliable PCR results from luminol treated bloodstains. 2000

Della Manna, A. and Montpetit, S.

Notes: In this study, the PowerPlex® 1.1 System was used for STR typing of DNA isolated from luminol treated bloodstains. (2252)

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Forensic Sci. Int. 129, 43–50. A report of the 2000 and 2001 paternity testing workshops of the English Speaking Working Group of the International Society for Forensic Genetics. 2000

Hallenberg, C. and Morling, N.

Notes: These authors present the results of the 2000 and 2001 Paternity Testing Workshops, which were held to compare DNA typing results and laboratory practices between laboratories. Participating labs received a questionnaire about their processes and blood samples for DNA analysis. The results were compiled, and 91% (2000) and 86% (2001) of labs used PCR-based methods for DNA typing, and typing errors occurred in 0.3% (2000) and 0.1% (2001) of the submitted results. The PCR-based STR-typing kits used in this study included the PowerPlex® 16 System and F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein). (3834)

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J. Clin. Microbiol. 38(8), 2829-2836. Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon 2000

Park, S., Wong, M., Marras, S. A. E., Cross, E.W., Kiehn, T.E., Chaturvedi, V., Tyagi, S., and Perlin, D.S.

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

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J. Forensic Sci. 45(3), 677-683. Validation of the PowerPlex 1.1™ loci for use in human identification. 2000

Greenspoon, S.A., Lytle, P.J., Turek, S.A., Rolands, J.M., Scarpetta, M.A. and Carr, C.D.

Notes: This paper describes validation of Promega's PowerPlex® 1.1 System. (2259)

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Appl. Environ. Microbiol. 65, 2369-2375. High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. 1999

Duim, B., Wassenaar, T.M., Rigter, A., Wagenaar, J.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 109 cells/ml. The isolated DNA was used for AFLP analysis. (1203)

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Genetics 151, 785-795. Multiple-trait quantitative trait loci analysis using a large mouse sibship. 1999

Jackson, A.U., Fornes, A., Galecki, A., Miller, R.A., Burke, D.T.

Notes: 20ng mouse genomic DNA was amplified with primers specific for 83 SSLP (single sequence length polymorphism) marker loci for genome-wide genotyping. Amplification products were separated on a denaturing gel and were visualized by silver staining using the SILVER SEQUENCE™ DNA Sequencing System. (0965)

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