We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Citations Search

Sort By:

Virchows Arch Epub before print. DNA degrades during storage in formalin-fixed and paraffin-embedded tissue blocks 2017

Guyard, A., Boyez, A., Pujals, A., Robe, C., Tran Van Nhieu, J., Allory, Y., Moroch, J., Georges, O., Fournet, J.C., Zafrani, E.S. and Leroy, K.

Notes: DNA was purified from 46 carcinoma FFPE blocks using the same method that was used ~5.5 years after the samples were originally studied. The purified DNA was quantified and showed on average a 53% decline in the amount of DNA recovered from each sample. Amplifiability of purified DNA over time was determined by the ProNex® DNA QC Assay. ProNex® DNA QC Assay results showed that length of amplifiable DNA fragments decreased over time by comparing the ratio of 75bp fragments to 150bp and 300bp fragments respectively. The ratio of 75bp to 150bp fragments showed a decrease in amplifiable DNA from 58% to 16% over time, while the ratio of 75bp to 300bp fragments showed a decrease in amplifiable DNA from 31% to 2%. The decrease in amplifiable DNA fragments lead to a 3.3 fold decrease in library preparation yield. (4879)

Expand Full Notes »

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom