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J. Neurosci. 17, 6094-6104.. Detection of functional nicotinic receptors blocked by alpha-bungarotoxin on PC12 cells and dependence of their expression on post-translational events. 1997

Blumenthal, E.M., Conroy, W.G., Romano, S.J., Kassner, P. D. and Berg, D. K.

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM®-T Vector and sequenced. (1419)

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J. Clin. Invest. 99(5), 838-845. Differential expression of Exons 1a and 1c in mRNAs for sterol regulatory element binding Protein-1 in human and mouse organs and cultured cells. 1997

Shimomura, I., Shimano, H., Horton, J., Goldstein, J. and Brown, M.

Notes: The pGEM®-T Vector System was used in this study. (2009)

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Proc. Natl. Acad. Sci. USA 94, 2563-2568. EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia. 1997

So, C.W., Caldas, C., Liu, M.M., Chen, S.J., Huang, Q.H., Gu, L.J., Sham, M.H., Wiedemann, L.M. and Chan, L.C.

Notes: Promega's pGEM®-T Vector System and TNT® Coupled Reticulocyte Lysate System were used in this study. (1948)

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J. Neurosci. 17, 9145-9156. Estradiol Enhances Prostaglandin E2 Receptor Gene Expression in Luteinizing Hormone-Releasing Hormone (LHRH) Neurons and Facilitates the LHRH Response to PGE2 by Activating a Glia-to-Neuron Signaling Pathway 1997

Rage, F., Lee, B.J., Ma, Y. J., Ojeda, S. R.

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM®-T Vector System. (0529)

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Proc. Natl. Acad. Sci. USA 94, 4211-4216. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6- desaturated fatty acids in transgenic tobacco. 1997

Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R., Napier, J.A.

Notes: The authors use the Reverse Transcription System, Wizard® PCR Preps DNA Purification Systems, Wizard® Plus Minipreps DNA Purification System and the pGEM®-T Vector System in their studies. (0445)

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J. Neurosci. 17, 6678-6684. Expression of zinc transporter gene, ZnT-1, is induced after transient forebrain ischemia in the gerbil. 1997

Tsuda, M., Imaizumi, K., Katayama, T., Kitagawa, K., Wanaka, A., Tohyama, M., Takagi, T.

Notes: PCR products generated by differential display analysis were subcloned into the pGEM®-T Vector System and subsequently sequenced. (0240)

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Proc. Natl. Acad. Sci. USA 94, 2415-2420. Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. 1997

Munster, T., Pahnke, J., Di Rosa, A., Kim, J.T., Martin, W., Saedler, H., Theissen, G.

Notes: The 3'-RACE products were cloned with the pGEM®-T Vector System. (0673)

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Am. J. Physiol. 273, C1937-C1946. Functional and molecular characterization of NHE3 expression during ontogeny in rat jejunal epithelium. 1997

Collins, J.F., Xu, H., Kiela, P.R., Zeng, J., Ghishan, F.K.

Notes: The pTargeT™ Vector was used to subclone the 2.5kb Na+/H+ Exchanger 2 and the 2.7kb Na+/H+ Exchanger 3 directly from PCR reactions. The constructs were stably transfected into PS120 Chinese hamster lung fibroblasts and selected for resistance to the neomycin analog, G-418. After 7-10 days of selection, the cells were further tested for expression by RT-PCR and immunoblotting. (1318)

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Neuron 18, 153-166. Identification of functionally distinct isoforms of the n-type Ca2+ channel in rat sympathetic ganglia and brain. 1997

Lin, Z., Haus, S., Edgerton, J. and Lipscombel, D.

Notes: The pGEM®-T Vector System was used in this study. (1969)

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J. Exp. Biol. 185, 1005-1012. Interleukin-18 (interferon-gamma-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage colony-stimulating factor and not via interferon-gamma to inhibit osteoclast formation. 1997

Udagawa, N., Horwood, N.J., Elliott, J., Mackay, A., Owens, J., Okamura, H., Kurimoto, M., Chambers, T.J., Martin, T.J., Gillespie, M.T.

Notes: PCR products were cloned into pGEM®-T Vector System. (0250)

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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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Proc. Natl. Acad. Sci. USA 94, 5273-5277. Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor. 1997

Baier, M., Bannert, N., Werner, A., Lang, K. and Kurth, R.

Notes: To identify the true transcriptional start of the IL-16 mRNA methods for rapid amplification of cDNA ends were applied. The complete pro-IL-16 cDNA was cloned, sequenced, and expressed in COS-7 cells. The authors used the pGEM®-T Vector System to clone fragments that were then used as templates in the Riboprobe® in Vitro Transcription System. (1453)

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J. Neurosci. 17, 8156-8168. Nervous system-specific expression of a novel serine protease: Regulation in the adult rat spinal cord by excitotoxic injury 1997

Scarisbrick, I.A., Towner, M.D., Isackson, P.J.

Notes: PolyA+ RNA was isolated from rat total RNA using the PolyATtract® mRNA Isolation System and used for Northern analysis. The pGEM®-T Vector System was used to clone products from RT-PCR. (0447)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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Proc. Natl. Acad. Sci. USA 94, 4149-4154. Target-independent cholinergic differentiation in the rat sympathetic nervous system. 1997

Schäfer, M., Schütz, B., Weihe, E. and Eiden, L.

Notes: The pGEM®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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J. Clin. Invest. 98(1), 43-49. Acute regulation by insulin of phosphatidylinositol-3-kinase, Rad, Glut 4, and lipoprotein lipase mRNA levels in human muscle. 1996

Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.

Notes: Promega's Tth DNA Polymerase, pGEM®-T Vector System and Riboprobe® System were used in this study. (2002)

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Proc. Natl. Acad. Sci. USA 93, 14548-14553. Characterization of serum amyloid A protein mRNA expression and secondary amyloidosis in the domestic duck. 1996

Guo, J., Aldrich, C., Mason, W. and Fox, J.

Notes: The pGEM®-T Vector System was used in this study. (2194)

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Proc. Natl. Acad. Sci. USA 93, 13036-13041. Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish. 1996

Zardoya, R. , Abouheif, E. , Meyer, A.

Notes: PCR products were cloned into the pGEM®-T Vector System. (0082)

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Proc. Natl. Acad. Sci. USA 93, 15244-15248. Independent evolution of the prochlorophyte and green plant chlorophyll a/b light-harvesting proteins. 1996

Roche, J. van der Staay, G., Partensky, F., Ducret, A., Aebersold, R., Li, R., Golden, S., Hiller, R., Wrench, R., Larkum, A. and Green, B.

Notes: The pGEM®-T Vector System was used in this study. (2005)

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Proc. Natl. Acad. Sci. USA 93, 13014-13019. Inhibition of granulocytic differentiation by mNotch1. 1996

Milner, L., Bigas, A., Kopan, R., Brashem-Stein, C., Bernstein, I. and Martin, D.

Notes: The pGEM®-T Vector System was used in this study. (1970)

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Proc. Natl. Acad. Sci. USA 93, 13020-13023. Pinus banksiana has at least seven expressed alcohol dehydrogenase genes in two linked groups. 1996

Perry, D. and Furnier, G.

Notes: The pGEM®-T Vector System was used in this study to clone amplified cDNAs. (1973)

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Proc. Natl. Acad. Sci. USA 93, 15394-15399. Prevention of hepatitis C virus infection in chimpanzees by hyperimmune serum against the hypervariable region 1 of the envelope 2 protein. 1996

Farci, P., Shimoda, A., Wong, D., Cabezon, T., DeGioannis, D., Strazzera, A., Shimizu, Y., Shapiro, M., Alter, H., Purcell, R.

Notes: The pGEM®-T Vector System was used in this study. (2193)

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Proc. Natl. Acad. Sci. USA 93, 12885-12889. RGS-r, a retinal specific RGS protein, binds an intermediate conformation of transducin and enhances recycling. 1996

Chen, C., Wieland, T., Simon, M.

Notes: The pGEM®-T Vector System was used in this study. (1967)

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Cell 87, 1181-1190. Vascular dysmorphogenesis caused by an activating mutation in the receptor tyrosine kinase TIE2 1996

Vikkula, M. , Boon, L. M. , Carraway, K. L. , Calvert, J. T. , Diamonti, A. J. , Goumnerov, B. , Pasyk, K. A. , Marchuk, D. A. , Warman, M. L. , Cantley, L. C. , Mulliken, J. B. , Olsen, B. R.

Notes: A 3,375 bp fragment was cloned in the pGEM®-T Vector System. (0223)

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