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Int. Congr. Ser. 1239, 165–9. Allele distribution of 15 STRs in a population from Extremadura (Central-Western Spain). 2003

García-Hirschfeld, J. Farfán, M.J., Prieto, V., López-Soto, M., Torres, Y. and Sanz, P.

Notes: The authors generated population data from unrelated individuals in Extremadura in western Spain using the PowerPlex® 16 System and the AmpFlSTR® Profiler Plus™, COfiler® and SGM Plus® kits. DNA was collected as blood samples and isolated by phenol:chloroform extraction or with GFX genomic purification columns. Amplifications were performed as directed by the manufacturer, and amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. (3844)

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Forensic Sci. Int. 135, 60-63. Allele frequencies for 15 STR loci in Van-Agri districts of the Eastern Anatolia region of Turkey. 2003

Cakir, A.H., Celebioglu, A., Altunbas, S. and Yardimci, E.

Notes: The PowerPlex® 16 System was used to type 116 individuals from the Van-Agri districts of the Eastern Anatolia region of Turkey. The study reports allele frequencies for this population as well as other statistical data. (2822)

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Forensic Sci. Int. 136, 86-88. Allelic frequencies at 12 STR loci in Colombian population. 2003

Benítez-Páez, A. and Reyes, H.O.

Notes: Allele frequencies for a Mestizo population in Columbia were determined with the PowerPlex® 16 System. DNA was purified using the Wizard® Genomic DNA Purification Kit.  (3003)

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Int. Congr. Ser. 1239, 673–6. Evaluation of “in house” criteria for PCR-based analysis in immigration casework. 2003

van Eede, P.H., Keller, S. and de Lange, G.G.

Notes: The authors evaluated criteria for using STR analysis to resolve immigration cases by examining 156 one-parent child combinations and 105 two-parent child combinations from 28 families. STR analyses were performed in duplicate using the SMG Plus® kit and a combination of CTTv and FFFL Multiplexes. The authors concluded that the combination of these kits, which analyze a total of 21 STR loci, can be used to successfully resolve immigration casework. However, in 9% of the one-parent cases their criteria of a parental index of 1.000 or more was not met. Additional testing using the PowerPlex® 16 System reduced this from 9% to 1 out of 156 indices. (3858)

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Int. Congr. Ser. 1239, 609–11. Evaluation of Powerplex™ 16 for typing of degraded DNA samples. 2003

Glock, B., Reisacher, R.B.K.. Rennhofer, S.O., Tröscher, D., Dauber, E.M. and Mayr, W.R.

Notes: The authors evaluated the ability of the PowerPlex® 16 System to amplify degraded DNA samples and compared the results with those obtained using the AmpFlSTR® SGM Plus® kit. They amplified DNA from six whole blood samples that had been stored at 4°C or room temperature for 20 days, DNA isolated from six pairs of archived whole blood samples, and from corresponding plasma samples that had been stored below –20°C for 1–3 years and were assumed to be degraded. Amplifications were assembled and performed using protocols optimized in their laboratory. For the whole blood and plasma samples stored below –20°C, the PowerPlex® 16 System gave eight full profiles and the SGM Plus® kit gave six full profiles. No differences were observed between the two kits with DNA extracts stored at 4°C or room temperature. As expected, the frequency of allele dropout increased as the amplified fragment length increased. (3876)

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Forensic Sci. Int. 134, 225-231. Genetic diversity in four tribal groups of western India: a survey of polymorphism in 15 STR loci and their application in human identification. 2003

Gaikwad, S. and Kashyap, V.K.

Notes: The PowerPlex™ 16 System was used to type 426 people from 4 tribal groups in Western India. The allele frequencies for the PowerPlex™ 16 System loci are provided for each of the tribal groups. (3011)

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Forensic Sci. Int. 132, 216-222. Genetic polymorphism revealed by 13 tetrameric and 2 pentameric STR loci in four Mongoloid tribal population 2003

Maity, B., Nunga, S.C. and Kashyap, V.K.

Notes: The PowerPlex® 16 System was used to genetically type 354 people from four distinct tribes (Hmar, Lai, Lusei, and Mara) in Northeastern India. Allele frequencies and population statistics are provided.  (3004)

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Forensic Sci. Int. 135, 64-66. Genetic profile of the Ecuadorian Mestizo population (Ecuador-South America) by using the Power Plex 16 System Kit. 2003

Gonzalez-Andrade, F., Sanchez, D. and Martinez-Jarreta, B.

Notes: The PowerPlex® 16 System was used to type 317 Mestizos in Ecuador.  Allele frequencies and statistical analysis of the data are provided. (2821)

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Int. Congr. Ser. 1239, 131–5. Multiplex STR genotyping: comparison study, population data and new sequence information. 2003

Alves, C., Gusmão, L., Pereira, L. and Amorim, A.

Notes: The authors generated allele frequency data for 100 unrelated individuals from North Portugal and 110 unrelated individuals from Mozambique using the PowerPlex® 16 System, GenePrint® CTTv Multiplex and Profiler Plus™ kit. Four inconsistencies in genotypes between the kits were detected, three for D5S818 and 1 for D8S1179, due to primer-binding site mutations. (3862)

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Int. Congr. Ser. 1239, 565–7. Mutation rates at 23 different short tandem repeat loci. 2003

Dauber, E.M., Bär, W., Klintschar, M., Neuhuber, F., Parson, W., Glock, B. and Mayr, W.R.

Notes: The authors examined almost 24,000 meioses to calculate the mutation rates at 23 STR loci, including CD4, CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1132, D8S1179, D12S391, D13S317, D16S539, D17S976, D18S51, D19S433, D21S11, F13A1, F13B, FES, FGA, SE33, TH01, TPOX and VWA. DNA was collected as blood samples or buccal swabs, isolated and amplified using the Powerplex™ 16 System, Profiler™, Profiler Plus™, COfiler™ or SGM Plus™ kit or singleplex amplification. Their results confirm that loci with long uninterrupted repeats have higher mutation rates. (3865)

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Forensic Sci. Int. 133, 250-253. Polymorphism of 17 STRs by multiplex analysis in Japanese population. 2003

Hashiyada, M., Itakura, Y., Nagashima, T., Nata, M. and Funayama, M.

Notes: The authors used the PowerPlex® 16 System to type 526 Japanese individuals. Allele frequencies for 13 CODIS loci were determined with the PowerPlex® 16 System and AmpFlSTR® Identifiler® kits. (2823)

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Forensic Sci. Int. 138, 116–8. Population data for 11 STR loci in northeast China Han. 2003

Zhang, Y.J., Xu, Q.S. and Lee, J.B.

Notes: Allele frequencies for 11 autosomal STR loci were determined in the Yan Bian region of China. Population data for nine STR loci were determined using the AmpFlSTR® Profiler Plus™ kit and for the Penta D and Penta E loci using the PowerPlex® 16 System. DNA was isolated using phenol:chloroform extraction, and 1ng was amplified as directed by the manufacturer. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3826)

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Forensic Sci. Int. 138, 116–118. Population data for 11 STR loci in northeast China Han. 2003

Zhanga, Y.J., Xub, Q.S. and Lee, J.B.

Notes: In this paper, researchers report allele frequencies for a population in Northeastern China. Data was generated using the PowerPlex® 16 System. DNA from volunteers was extracted using a phenol chloroform method. (3000)

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Int. Congr. Ser. 1239, 243–4. Population data from Chile using PowerPlex-16. 2003

Celis, R., Aguirre, E., Lorente, J.A and Jorquera, H.C.

Notes: The authors generated population data from 323 unrelated individuals in Chile using the PowerPlex® 16 System. All loci were shown to be highly polymorphic. (3864)

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Int. Congr. Ser. 1239, 121–2. Population genetic analysis in Hungarian populations using the Powerplex™ 16 system. 2003

Egyed, B., Füredi, S., Angyal, M., Balogh, I., Kalmár, L., Tordai, A., Woller, J. and Pádár, Z.

Notes: The authors generated population data for 723 individuals living in four regions of Hungary. DNA was collected as blood samples and amplified using the PowerPlex® 16 System. Amplified products were detected using the ABI PRISM® 377 DNA Sequencer or 310 Genetic Analyzer. (3866)

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Int. Congr. Ser. 1239, 235–8. Portuguese population data on two pentanucleotide STR loci Penta E and Penta D. 2003

Ribeiro, T., Viriato, L., Vieira-Silva, C., Cruz, C., Espinheira, R. and Geada, H.

Notes: The authors reported population data for the Penta E and Penta D loci in 160 Portuguese Caucasians, 19 Portuguese of African origin and 150 meioses. DNA was extracted from blood samples using Chelex® resin, then purified using the Wizard® DNA Clean-Up System. Amplifications were performed using the PowerPlex® 16 System, the SGM Plus® kit and the GeneAmp® PCR System 9600. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3845)

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Int. Congr. Ser. 1239, 125–30. PowerPlex™ 16 analysis in the Japanese population. 2003

Hashiyada, M., Itakura, Y. and Nata, M.

Notes: The authors generated population data from 508 unrelated Japanese individuals using the PowerPlex® 16 System. DNA was collected as buccal swabs and isolated using Chelex® resin followed by phenol:chloroform extraction and ethanol precipitation. For each PowerPlex® 16 reaction, 2ng of DNA was amplified using a GeneAmp® PCR System 9700, and amplified products were detected using the ABI PRISM® 377 DNA Sequencer. (3843)

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Forensic Sci. Int. 134, 222-224. STR data for PowerPlex 16 System from Buenos Aires population, Argentina. 2003

Berardi, G., Toscanini, U. and Raimondi, E.

Notes: The PowerPlex® 16 System was used to report allele frequencies for a population of people living in Capital Federal and in the Buenos Aires Metropolitan Area. (2999)

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Forensic Sci. Int. 135, 75-78. STR frequencies for the PowerPlex 16 System Kit in a population from Northeast Spain. 2003

Paredes, M., Crespillo, M., Luque, J.A. and Valverde, J.L.

Notes: The PowerPlex® 16 System was used to type 204 Caucasian individuals from Northeastern Spain.  Allele frequencies and statistical analyses of the data are provided. (2820)

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J. Forensic Sci. 47, 773-785. Validation of a 16-locus fluorescent multiplex system. 2003

Krenke, B.E., Tereba, A., Anderson, S.J., Buel, E., Culhane, S., Finis, C.J., Tomsey, C.S., Zachetti, J.M., Masibay, A., Rabbach, D.R., Amiott, E.A. and Sprecher, C.J.

Notes: In this paper, researchers from both Promega and state crime laboratories provide an evaluation of the Powerplex® 16 System loci (CODIS, Penta D, Penta E, and amelogenin). Data from several laboratories using a variety of thermal cyclers and the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer are presented. The Powerplex® 16 System was able to consistently genotype samples from as little as 0.0625-2ng of genomic DNA template. Other factors such as reaction volume, annealing temperature, titration of AmpliTaq Gold® DNA Polymerase, magnesium, or primer pairs, and 1ng DNA mixtures were analyzed using the Powerplex® 16 System.  The researchers also present data on stutter analysis and cross reactivity of primer sets on primate DNA templates for each loci. (2675)

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J. Forensic Sci. 48, 358–61. Variant alleles on the penta E locus in the PowerPlex 16 kit. 2003

Mizuno, N., Sekiguchi, K., Sato, H. and Kasai, K.

Notes: The authors identified two microvariant alleles (18.4 and 19.4) at Penta E in the Japanese population. Both microvariant alleles had a partial repeat motif of AAAA rather than the AAAGA repeat. The authors discuss their ability to resolve alleles differing in size by only one base (i.e., 18.4 and 19) by capillary electrophoresis. (3970)

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Forensic Sci. Int. 126, 178–86. Allele frequencies for STR loci of the Powerplex 16 multiplex system in five endogamous populations of India. 2002

Kashyap, V.K., Sarkar, N. and Trivedi. R.

Notes: Population data for five populations in India were determined using the PowerPlex® 16 System. Blood was collected from 95 unrelated individuals, and DNA was isolated by phenol:chloroform extraction. Two nanograms was amplified, and amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3831)

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J. Forensic Sci. 47(4), 757-772. Characterization and validation studies of PowerPlex® 2.1, a nine-locus short tandem repeat (STR) multiplex system and Penta D monoplex. 2002

Levedakou, E.N., Freeman, D.A, Budzynski, M.J., Early, B.E., Damaso, R.C., Pollard, A.M., Townley, A.J., Gombos, J.L., Lewis, J.L., Kist, F.G., Hockensmith, M.E., Terwilliger, M.L., Amiott, E., McElfresh, K.C., Schumm, J.W., Ulery, S.R., Konotop, F., Sessa, T.L., Sailus, J.S., Crouse, C.A., Tomsey, C.S., Ban, J.D. and Nelson, M.S.

Notes: This study provides a multi-lab validation of the PowerPlex® 2.1 and Penta D systems. The paper also reveals the primer sequences for all ten loci. Stutter, species cross-reactivity, environmental variations, and sample mixtures were examined for all loci. The researchers were able to consistently amplify from 0.75ng of sample DNA.  (2679)

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Forensic Sci. Int. 126, 173–7. Distribution of alleles of 15 STR loci of the Powerplex 16 Multiplex system in four predominant population groups of South India. 2002

Rajkumar, R. and Kashyap, V.K.

Notes: The authors examined allele frequencies for 15 autosomal STR loci in four population groups in southern India using the PowerPlex® 16 System. DNA was isolated from blood using phenol:chloroform extraction, 2ng was amplified and amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3833)

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Forensic Sci. Int. 128, 196–201. Genetic diversity at two pentanucleotide STR and thirteen tetranucleotide STR loci by multiplex PCR in four predominant population groups of central India. 2002

Sarkar, N. and Kashyap, V.K.

Notes: Population data was determined for 208 unrelated individuals from central India using the PowerPlex® 16 System. DNA was isolated from blood by organic extraction, and 2ng was amplified per reaction. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3829)

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