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Legal Med. (Tokyo) 7, 314–318. DNA typing from skeletal remains following an explosion in a military fort--first experience in Ecuador (South-America). 2005

González-Andrade, F. and Sánchez, D.

Notes: On November 20, 2002, an explosion in a munitions facility left 7 people dead, 100 injured and 5 missing. The authors used DNA typing to identify 2 tissue samples and 19 bone samples. These samples, as well as reference samples from relatives of the missing persons, were analyzed using the PowerPlex® 16 System. DNA was extracted using phenol:chloroform:isoamyl alcohol extraction and concentrated, then amplified using 28–30 cycles. For bone samples, the cycle number was increased to 35. The success rate was 90% (19 of 21 samples identified; 2 of 21 samples had a high degree of contamination and could not be identified). (3819)

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Forensic Sci. Int. 149, 109–13. Genetic variability of 16 Y-chromosome STRs in a sample from Equatorial Guinea (Central Africa). 2005

Arroyo-Pardo, E., Gusmão, L., López-Parra, A.M., Baeza, C., Mesa, M.S. and Amorim, A.

Notes: The authors collected DNA samples from 101 donors from Equitorial Guinea. The Y-STR haplotypes at 12 loci were determined using the PowerPlex® Y System (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Amplification products were detected using an ABI PRISM® 310 genetic analyzer. The haplotypes of five other loci (DYS460, DYS461, GATA A10, GATA C4 and GATA H4) were also determined. Haplotype and alelle frequencies and haplotype diversity were calculated. A total of 94 haplotypes were obtained.
(3653)

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Croat. Med. J. 46, 563–577. Improving efficiency of a small forensic DNA laboratory: validation of robotic assays and evaluation of microcapillary array device. 2005

Crouse, C.A., Yeung, S., Greenspoon, S., McGuckian, A., Sikorsky, J., Ban, J. and Mathies, R.

Notes: The authors validated the DNA IQ™ System for manual and automated DNA purification from blood, tissue, bone, hair, epithelial cells and mixed stains such as semen. Automated DNA extraction was performed using the Beckman Coulter Biomek® 2000 workstation. Purified DNA (0.5–1.0ng) was then amplified using the PowerPlex® 16 BIO System and a 6% PAGE PLUS™ gel. Automated DNA purification and use of a single-amplification STR system greatly increased sample throughput. (3645)

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DNA IQ™ System

Forensic Sci. Int. 148, 239–42. STR allelic frequencies for an African population sample (Equatorial Guinea) using AmpFlSTR Identifiler and Powerplex 16 kits. 2005

Alves, C., Gusmão, L., López-Parra, A.M., Soledad Mesa, M., Amorim, A. and Arroyo-Pardo, E.

Notes: Population data were generated for a group of 134 unrelated individuals from Equatorial Guinea using the PowerPlex® 16 System and the AmpFlSTR® Identifiler® kit. DNA was extracted using Chelex® resin, amplified as directed by the manufacturer, and amplified products detected using an ABI PRISM® 310 Genetic Analyzer. (3837)

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Forensic Sci. Int. 150, 1–15. Twelve short tandem repeat loci Y chromosome haplotypes: genetic analysis on populations residing in North America. 2005

Budowle, B., Adamowicz, M., Aranda, X.G., Barna, C., Chakraborty, R., Cheswick, D., Dafoe, B., Eisenberg, A., Frappier, R., Gross, A.M., Ladd, C., Lee, H.S., Milne, S.C., Meyers, C., Prinz, M., Richard, M.L., Saldanha, G., Tierney, A.A., Viculis, L. and Krenke, B.E.

Notes: The Y-STR haplotypes of 2443 male individuals in North America were determined, and allele frequencies were compiled for African-Americans, Asians, Caucasians, Hispanics and Native Americans. The information presented includes haplotype frequencies, mutation rates, genetic diversity, population substructure effects and concordance. Y-STR haplotypes were determined using the PowerPlex® Y System. DNA samples were obtained as liquid whole blood, buccal swabs or dried blood on FTA® blood cards and extracted using phenol-chloroform extraction, a salting out method, Chelex®, the DNA IQ™ System or the QIAamp blood kit. Amplifications contained 0.5–1.0ng of purified DNA or a 1.2mm FTA® punch. Amplification products were detected using the ABI PRISM® 377 DNA sequencer or the ABI PRISM® 310 or 3100 genetic analyzer. (3638)

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Croat. Med. J. 46, 530–539. Twelve-year experience in identification of skeletal remains from mass graves. 2005

Andelinovic, S., Sutlovic, D., Erceg Ivkosic, I., Skaro, V., Ivkosic, A., Paic, F., Rezic, B., Definis-Gojanovic, M. and Primorac D.

Notes: These authors used DNA typing to identify human skeletal remains found in mass graves. DNA was isolated using standard phenol/chloroform extraction, the DNA IQ™ System or other methods. A modified DNA IQ™ System protocol was developed using 2g of pulverized bone. DNA was quantitated using the AluQuant® Human DNA Quantitation System or Quanti-Blot™ Human DNA quantitation kit. DNA typing was performed using several STR amplification kits, including the PowerPlex® 16 System. In some cases mitochondrial DNA testing was necessary due to the degree of nuclear DNA degradation. Of the 481 samples, 385 were amplified successfully and 109 sets of remains were identified. (3640)

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Forensic Sci. Int. 151, 111–24. Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex. 2005

Krenke, B.E., Viculis, L., Richard, M.L., Prinz, M., Milne, S.C., Ladd, C., Gross, A.M., Gornall. T., Frappier, J.R., Eisenberg, A.J., Barna, C., Aranda, X.G., Adamowicz, M.S., and Budowle, B.

Notes: The authors present the results of 17 developmental validation studies for the PowerPlex® Y System, which amplifies 12 Y-STR loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. The authors examined the effect of DNA quality and quantity, as well as analysis variation on the system. Variation of the amplification conditions, including cycle number, reaction volume, DNA polymerase concentration, magnesium concentration, annealing temperature and thermal cycler used, was also examined. The average stutter, specificity, precision and limitations of the system were also reported. Finally, the results demonstrated that the protocol performed as described. (3656)

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J. Forensic Sci. 50, 239—41. Y-chromosomal haplotypes for the PowerPlex Y for twelve STRs in a Peruvian population sample. 2005

Iannacone, G.C., Tito, R.Y., Lopez, P.W., Medina, M.E., and Lizarraga, B.

Notes: The authors collected blood samples, stored on FTA® blood cards, from 79 unrelated Peruvian males and isolated DNA using an unspecified method. The purified DNA was amplified using the PowerPlex® Y System and a Mastercycler gradient thermal cycler (Eppendorf). Amplified products were detected using an ABI PRISM® 310 genetic analyzer. Allele frequencies and gene diversity were calculated. A total of 76 different haplotypes were observed. (3657)

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Forensic Sci. Int. 152, 95–99. Y-chromosomal STR haplotypes in three ethnic groups and one cosmopolitan population from Tunisia. 2005

Cherni, L., Pereira, L., Goios, A., Loueslati, B.Y., Khodjet el Khil, H., Gomes, I., Gusmão, L., Alves, C., Slama, A., Amorim, A. and Elgaaied, A.B.

Notes: The authors collected DNA from 247 unrelated males in three ethnic groups in Tunisia to determine Y-STR haplotype frequencies. DNA was amplified using the PowerPlex® Y System, and amplified products were detected using an ABI PRISM® 310 genetic analyzer. The authors compared haplotype frequencies for 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439) in these three ethnic groups to other nearby populations. (3646)

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Forensic Sci. Int. 155, 205–210. Y-chromosome STR haplotypes in Danes. 2005

Hallenberg, C., Nielsen, K., Simonsen, B., Sanchez, J. and Morling, N.

Notes: These authors collected blood samples from 185 Danish males and (as negative controls) 11 females. Purified DNA was amplified using the PowerPlex® Y System and 10/20 cycling. Amplification products were detected using an ABI PRISM® 3100 genetic analyzer. A total of 163 Y-STR haplotypes were observed, including 144 unique haplotypes. For the DYS392 locus, a 10.2 allele was observed. All female DNA samples failed to amplify. (3648)

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Forensic Sci. Int. 151, 317–321. Y-chromosome STR haplotypes in Somalis. 2005

Hallenberg, C., Simonsen, B., Sanchez, J. and Morling, N.

Notes: The authors collected blood samples from 201 male Somalis to determine haplotype frequencies for 12 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Female DNA was tested as a negative control. Purified DNA (0.7µl of extracted DNA) or a 1.1mm2 FTA® blood card punch was amplified using the PowerPlex® Y System and 10/16 cycling. Amplification products were detected using a ABI PRISM® 3100 genetic analyzer. A total of 96 haplotypes were observed, 71 of which were unique. (3649)

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Forensic Sci. Int. 141, 193–196. 17 STR data (AmpF/STR Identifiler and Powerplex 16 System) from Cabinda (Angola). 2004

Beleza, S., Alves, C., Reis, F., Amorim, A., Carracedo, A. and Gusmão, L.

Notes: The authors determined allele frequencies for 17 autosomal STR loci in an Angolan population using the PowerPlex® 16 System and the AmpFlSTR® Identifiler® kit. DNA was collected from 110 unrelated individuals living in Cabinda, Angola, extracted using Chelex® resin and amplified as directed by the manufacturers. Amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. The authors compared their population data with other African databases published in the scientific literature. (3824)

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J. Forensic Sci. 49, 754–759. A simple and efficient method for extracting DNA from old and burned bone. 2004

Ye, J., Ji, A., Parra, E.J., Zheng, X., Jiang, C., Zhao, X., Hu, L. and Tu, Z.

Notes: These authors used a new DNA purification method that combines cetyltrimethylammonium bromide (CTAB) and isoamyl alcohol/chloroform extraction to isolate DNA from bones soaked in water, burned bones, or bones that had been buried for a long time. Following the CTAB and isoamyl alcohol/chloroform extraction, PCR inhibitors were removed using the DNA IQ™ System or the QIAquick PCR Purification Kit. The authors preferred the DNA IQ™ System because of its speed and ease-of-use. (3642)

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Forensic Sci. Int. 140, 131–2. Allele frequencies data and statistic parameters for 16 STR loci-D19S433, D2S1338, CSF1PO, D16S539, D7S820, D21S11, D18S51, D13S317, D5S818, FGA, Penta E, TH01, vWA, D8S1179, TPOX, D3S1358-in the Rio de Janeiro population, Brazil. 2004

Góes, A.C., da Silva, D.A., Fonseca Gil, E.H., da Silva, M.T., Pereira, R.W. and de Carvalho, E.F.

Notes: The authors determined allele frequencies for 16 autosomal STR loci for 230–300 unrelated individuals in Rio de Janeiro using the PowerPlex® 2.1 System and the AmpFlSTR® Identifiler® kit. DNA was extracted from blood by organic extraction, amplified as directed by the manufacturers, and the amplification products detected using an ABI PRISM® 310 Genetic Analyzer. Penta E and D18S51 were the most polymorphic of the loci examined. (3825)

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Int. Congr. Ser. 1261, 142-4. Allele frequencies of 15 STR loci in a Spanish population. 2004

Carril, J.C., Ocaña, M.A., Sierra, O., Molino, A., Cospedal, R. and Puente, J.

Notes: Population data were generated for a group of 341 unrelated Spanish individuals using the PowerPlex® 16 System. DNA was collected as blood samples or buccal swabs and extracted using phenol:chloroform extraction. Amplifications were carried out as directed by the manufacturer, and amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. (3840)

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Int. Congr. Ser. 1261, 151–3. Analysis of 12 STR loci in Antioquia (Colombia) population sample. 2004

Bravo, M.L.J., Builes, J.J., de Pancorbo, M.M. and Moreno, M.A.

Notes: The authors generated population data for 244–546 unrelated individuals from Antioquia in Columbia. DNA was extracted from whole blood samples using a salting out protocol, then amplified as directed by the manufacturer. Amplified fragments were detected using the ABI PRISM® 310 Genetic Analyzer. (3857)

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J. Forensic Sci. 49, 29-39. Application of the BioMek 2000 Laboratory Automation Workstation and the DNA IQ System to the extraction of forensic casework samples. 2004

Greenspoon, S.A., Ban, J.D., Sykes, K., Ballard, E.J., Edler, S.S., Baisden, M., and Covington, B.L.

Notes: This paper discusses automated DNA purification from a variety of forensic casework samples using the DNA IQ™ System on a Beckman BioMek® 2000 Laboratory Automated Workstation.  DNA was purified from various forensic casework samples including vaginal swabs, blood stains, tissue and samples.  The researchers also tested diluted and contaminated samples and the ability of the DNA IQ™ System on the Beckman BioMek® 2000 to purify DNA from these samples.  The PowerPlex® 1.1 System was used as a representative forensic laboratory typing system to test the contaminant level in all of the DNA IQ™ purified DNA samples.  (3052)

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DNA IQ™ System

Forensic Sci. Int. 139, 201–5. Contribution for an African autosomic STR database (AmpF/STR Identifiler and Powerplex 16 System) and a report on genotypic variations. 2004

Alves, C., Gusmão, L., Damasceno, A., Soares, B. and Amorim, A.

Notes: The authors determined allele frequencies for 17 autosomal STR loci in 144 unrelated individuals from Mozambique using the PowerPlex® 16 System and the AmpFlSTR® Identifiler® kit. DNA was extracted using Chelex® resin, amplified as directed by the manufacturer, and amplification products detected using the ABI PRISM® 310 Genetic Analyzer. The population sampled did not have a combined matching probability or power of exclusion as high as those calculated for African-American samples. (3827)

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J. Forensic Sci. 49(1), 1-5. Evaluation of an automated liquid hybridization method for DNA quantitation. 2004

Hayn, S., Wallace, M.M., Prinz, M. and Shaler, R.C.

Notes: DNA was purified from blood stains and buccal swabs with DNA IQ™ System and two other comparative methods. A high throughput AluQuant® assay on the BioMek® 2000 was compared to a quantiblot method for quantifying human genomic DNA. DNA samples extracted with the DNA IQ™ System had less variability than the quantiblot method. The AluQuant® System showed a similar level of sensitivity, reproducibility and precision compared to the quantiblot method.  (3007)

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Int. Congr. Ser. 1261, 160–2. Extended polymorphism at STR-locus D5S818. 2004

Dülmer, M., Henke, L., Baur, M., Fimmers, R., Pick, E. and Henke, J.

Notes: When using the PowerPlex® 16 System for routine paternity casework, the authors observed two single parental exclusions at D5S818 due to reverse homozygosity. To explore this further, they typed 2514 persons who were homozygous at the locus D5S818 and their 1304 children using a singleplex with two different primer pairs and the AmpFlSTR® Identifiler®, Profiler Plus™ and Profiler 1 kits. The authors identified three one-base-pair substitutions, one of which was within the primer-binding site for the PowerPlex® 16 System D5S818 primer. All persons who showed this substitution within the primer-binding site, which the authors designated the *10 variant 4 allele, were of German origin. (3863)

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Forensic Sci. Int. 146 Suppl., S129–S131. Genetic study of 11 Y-STRs in the populations of Reggio Calabria, Catanzaro, Cosenza (Calabria--South of Italy). 2004

Barbaro, A., Cormaci, P., Falcone, G., Barbaro, A. and Rizzo, M.

Notes: Blood samples were collected from approximately 300 males in southern Italy. DNA was extracted, quantified using the AluQuant® Human DNA Quantitation System, and amplified using the PowerPlex® Y System. Amplification products were detected using the ABI PRISM® 310 genetic analyzer. The distribution of Y-STR haplotypes in three southern Italian populations was determined. (3639)

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Minerva Ginecol. 56(3), 189-96. Intracytoplasmic sperm injection. Accomplishments and qualms. 2004

Neri, Q.V., Tanaka, N., Wang, A., Katagiri, Y., Takeuchi, T., Rosenwaks, Z. and Palermo, G.D.

Notes: These authors assessed the risk of transmitting genetic defects to children after intracytoplasmic sperm injection (ICSI) for treatment of male factor infertility. Genomic DNA was isolated from blood of both parents and children using the Wizard® Genomic DNA Purification Kit. Multiplex amplification of 22 sequence tagged sites on the Y chromosome was then performed on 123 samples using the Y Chromosome Deletion Detection System, Version 1.1 and a prototype Multiplex E from Promega. (3117)

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Forensic Sci. Int. 134, 219–221. STR data for PowerPlex 16 System from Neuquen population, SW Argentina. 2004

Toscanini, U., Berardi, G. and Raimondi, E.

Notes: One hundred and eleven people from the Neuquen province in Argentina were typed with the PowerPlex™ 16 System. Allele frequencies for the population are provided. (3009)

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Int. Congr. Ser. 1239, 165–9. Allele distribution of 15 STRs in a population from Extremadura (Central-Western Spain). 2003

García-Hirschfeld, J. Farfán, M.J., Prieto, V., López-Soto, M., Torres, Y. and Sanz, P.

Notes: The authors generated population data from unrelated individuals in Extremadura in western Spain using the PowerPlex® 16 System and the AmpFlSTR® Profiler Plus™, COfiler® and SGM Plus® kits. DNA was collected as blood samples and isolated by phenol:chloroform extraction or with GFX genomic purification columns. Amplifications were performed as directed by the manufacturer, and amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. (3844)

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Forensic Sci. Int. 135, 60-63. Allele frequencies for 15 STR loci in Van-Agri districts of the Eastern Anatolia region of Turkey. 2003

Cakir, A.H., Celebioglu, A., Altunbas, S. and Yardimci, E.

Notes: The PowerPlex® 16 System was used to type 116 individuals from the Van-Agri districts of the Eastern Anatolia region of Turkey. The study reports allele frequencies for this population as well as other statistical data. (2822)

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