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Promega Corporation

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Brain Res. Dev. Brain Res. 119, 217-224.. Developmental expression of urinary bladder neurotrophic factor mRNA and protein in the neonatal rat. 2000

Vizzard, M.A., Wu, K.H., Jewett, I.T.

Notes: The amounts of neurotrophins NGF, NT-3 and NT-4 were examined in the bladders of Wistar rats at various times after birth up to adulthood. NGF ranged from 5ng per bladder (day 5) to10ng per bladder (day 15). NT-3 ranged from less than 1ng per bladder at postnatal day 30 to as high as about 18ng per bladder in adulthood. NT-4 ranged from about 1ng per bladder on day 30 to more than 40ng per bladder in adulthood. NGF, NT-3,  and BDNF protein levels were determined with the NGF Emax® ImmunoAssay System, NT-3 Emax® ImmunoAssay System, and BDNF levels Emax® ImmunoAssay System, respectively. Extraction procedures are detailed. (0228)

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J. Neurosci. 20, 9187-9194. Differential expression of plasticity-related genes in waking and sleep and their regulation by the noradrenergic system. 2000

Cirelli, C., and Tononi, G.

Notes: The acquisition of long-term memories is severely impaired during sleep. Levels of brain-derived neurotrophic factor were quantitated in rat cerebral cortex in sleep and in awaking using Promega's BDNF Emax® ImmunoAssay System to try to understand this phenomenon. (2313)

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Neurosci. Lett. 280, 220-222.. Differential neurotrophin levels in cerebrospinal fluid and their changes during development in newborn rat. 2000

Xia, Y.X., Ikeda, T., Xia, X.Y., Ikenoue, T.

Notes: The levels of BDNF, NT-3 and NGF were examined in the cerebrospinal fluid of newborn rats (80 per sample) from postnatal day 1-21. NGF levels were consistently higher than the other neurotrophins ranging from 136pg/ml (day 21) to 607pg/ml (day 17) depending upon the day tested. NT-3 was the next most abundant ranging from approximately 40pg/ml (day 19) to 144pg/ml (day 7). BDNF was the least abundant ranging from 19.2pg/ml (day 5) to as high as 65pg/ml (day 13). NGF was determined with the NGF Emax® ImmunoAssay System (NGF ELISA), NT-3 levels were determined with the NT-3 Emax® ImmunoAssay System (NT-3 ELISA), and BDNF levels were determined with the BDNF Emax® ImmunoAssay System (BDNF ELISA). (0167)

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Brain Res. Dev. Brain Res. 121, 97-107. Ethanol-induced alterations in the expression of neurotrophic factors in the developing rat central nervous system. 2000

Heaton, M.B., Mitchell, J.J., Paiva, M., Walker, D.W.

Notes: Brain-derived neurotrophic factor nerve growth factor, neurotrophin-3 levels were quantitated in neonatal and early postnatal rat hippocampus, septum, cortex/striatum and cerebellum using Promega's BDNF Emax® ImmunoAssay System, NGF Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively. (2312)

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Neuropharmacology 39, 449-462. GABAB receptor antagonists nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) but not neurtrophin-3 (NT-3) in brain and spinal cord of rats. 2000

Heese, K., Otten, U., Mathivet, P., Raiteri, M., Marescaux, C., Bernasconi, R.

Notes: Rats were injected with a GABAB receptor antagonists and neurotrophin levels were analyzed in the cortex, hippocampus, and spinal cord 24-, 48-, 72- and 96-hour postinjection. The BDNF levels rose up to 2.5-fold over control levels in all three regions and reached a maximum at 72 hours. NT-3 levels decreased to 50% of control values within 48 hours. The BDNF levels were measured with the BDNF Emax® ImmunoAssay System (BDNF ELISA), and NT-3 levels were measured with the NT-3 Emax® ImmunoAssay System (NT-3 ELISA). (1076)

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Neurology 54, 2009-2011. Increased CSF levels of nerve growth factor in patients with Alzheimer’s disease. 2000

Hock, C., Heese, K., Mueller-Spahn, F., Huber, P., Riesen, W., Nitsch, R.M., and Otten, U.

Notes: The levels of various nerve growth factors were quantitated in the cerebrospinal fluid from Alzheimer's disease patients, nondemented control subjects, and age-matched patients with major depression.  Brain-derived neurotrophic factor, neurotrophin-4, and neurotrophin-3 levels were determined using Promega's BDNF Emax® ImmunoAssay System, NT-4 Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively. (2317)

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Nat. Neurosci. 3, 350-357. Knocking the NT4 gene into the BDNF locus rescues BDNF deficient mice and reveals distinct NT4 and BDNF activities. 2000

Fan G., Egles C., Sun Y., Minichiello L., Renger J.J., Klein R., Liu G., and Jaenisch R.

Notes: Mice were genetically altered to replace the brain-derived neurotrophic factor coding sequence with the neurotrophin-4 sequence in mice. These mice were viable but exhibited reduced body weight, infertility and skin lesions. BDNF and NT-4 protein levels in mouse cerebral cortex and muscle were quantitated using Promega's BDNF Emax® ImmunoAssay System and NT-4 Emax® ImmunoAssay System . (2315)

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J. Neuroimmunol. 104, 116-23. Nerve growth factor antibody exacerbates neuropathological signs of experimental allergic encephalomyelitis in adult lewis rats. 2000

Micera, A., Properzi, F., Triaca, V., and Aloe, L.

Notes: BDNF levels in rats with experimental allergic encephalomyelitis were monitored in blood, spinal cord and brain stem tissues using Promega's BDNF Emax® ImmunoAssay System. (2322)

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J. Neurosci. 20, 8-21. Positive modulation of AMPA receptors increases neurotrophin expression by hippocampal and cortical neurons. 2000

Lauterborn, J.C., Lynch, G., Vaderklish, P., Arai, A., Gall, C.M.

Notes: The BDNF Emax® ImmunoAssay System was used to quantitate BDNF levels in rat hippocampal slices. The slices were homogenized in the recommended lysis buffer and acid-activated prior to assay. Treatments with ampakine increased BDNF protein levels within 6 hours of exposure. (0846)

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Brain Res. Dev. Brain Res. 121, 157-167. Sex-specific effects of in utero manipulation of GABA(A) receptors on pre- and postnatal expression of BDNF in rats. 2000

Kellogg, C.K., Yao, J., and Pleger, G.L.

Notes: Late gestational exposure of rats to diazepam affects the developmental appearance of BDNF in the brain; the effects of the exposure were sex and region specific. Brain-derived neurotrophin factor protein levels were quantitated using Promega's BDNF Emax® ImmunoAssay System. (2314)

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J. Neurosci. 19, 3277-3286. Brain-derived neurotrophic factor mediates the anti-apoptotic effect of NMDA in cerebellar granule neurons: Signal transduction cascades and site of ethanol action. 1999

Bhave, S.V., Ghoda, L., Hoffman, P.L.

Notes: The level of BDNF in cerebellar granule cells after various treatments was assessed with the BDNF Emax® ImmunoAssay System. Cell extracts were prepared using the lysis buffer recommended in the technical manual, and BDNF levels were determined following acid treatment. BDNF levels increased with the amount of NMDA and also with the length of exposure to NMDA. (1445)

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Am. J. Respir. Cell Mol. Biol. 21 (4), 537-546. Cellular sources of enhanced brain-derived neurotrophic factor production in a mouse model of allergic inflammation. 1999

Braun, A., Lommatzsch, M., Mannsfeldt, A., Neuhaus-Steinmetz, U., Fischer, A., Schnoy, N., Lewin, G.R. and Renz, H.

Notes: The BDNF Emax® ImmunoAssay System was used to measure BDNF in cell-free bronchoalveolar lavage fluid (BALF) from ovalbumin sensitized BALB/c mice. Data was presented as picograms per milliliter of bronchoalveolar lavage fluid. Higher levels of BDNF were found in the bronchoalveolar lavage fluid of ovalbumin-sensitized mice. (2739)

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J. Immunol. 162, 6303-6306. Cutting Edge: Clonally restricted production of the neurotrophins brain-derived neurotrophic factor and neurotrophin-3 mRNA by human immune cells and Th1/Th2-polarized expression of their receptors. 1999

Besser, M. and Wank, R.

Notes: Strong expression of BDNF was detected in immune cells. The BDNF Emax® ImmunoAssay System was used to determine whether sufficient BDNF was produced for detection in cell culture medium. Media from untreated and irradiated cells (500,000 cells in 2ml of media) was assayed for BDNF content at 1 and 3 days. EBV-transformed lymphoblastoid B cell lines (B-LCL), MM1 monocytes and cloned T cells produced BDNF ranging from below detection to as high as 100pg/ml. Irradiated cells produced more BDNF than non-irradiated cells. (1441)

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Neurosci. Lett. 263, 149-152. Decreased accumulation of endogenous brain-derived neurotrophic factor against constricting sciatic nerve ligatures in streptozotocin-diabetic and galactose-fed rats. 1999

Mizisin, A.P., DiStefano, P.S., Liu, X., Garrett, D.N., Tonra, J.R.

Notes: Sciatic nerves of diabetic rats were excised and cut into three 1cm segments with regard to proximal, middle and distal from a ligature. Segments were homogenized in a small volume of lysis buffer and assayed for BDNF content with the BDNF Emax® ImmunoAssay System (BDNF ELISA). A lot of detail is provided for tissue preparation prior to assay. (0697)

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J. Neurosci. 19, 10-20. Neuroprotection and neuronal differentiation studies using substantia nigra dopaminergic cells derived from transgenic mouse embryos 1999

Son, J.H., Chun, H.S., Joh, T.H., Cho, S., Conti, B., Lee, J.W.

Notes: The authors developed a stable substantia nigra dopaminergic neuronal cell line via transgenic mice. The BDNF Emax® ImmunoAssay System was used to detected the secretion of neuroprotective BDNF into conditioned media following treatments with MPP+, H2O2, SNP, glutamate and TNFalpha. MPP+, H2O2 and the NO producer SNP caused a decrease in BDNF secretion and Glutamate and TNF alpha caused significant increases in secretion. (0353)

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J. Neurosci. 19, 5919-5931. Overexpression of brain-derived neurotrophic factor enhances sensory innervation and selectively increases neuron number. 1999

LeMaster, A.M., Krimm, R.F., Davis, B.M., Noel, T., Forbes, M.E., Johnson, J.E., Albers, K.M.

Notes: The BDNF Emax® ImmunoAssay was used to assess the levels of BDNF in various normal and transgenic mouse tissues: skin, trigeminal ganglia, nodose-petrosal ganglia and SCG. Good detail is provided for tissue preparation. (BDNF ELISA) (0827)

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Eur. J. Neurosci. 11, 1668-1676. The role of p75NTR in modulating neurotrophin survival effects in developing motoneurons. 1999

Wiese, S., Metzger, F., Holtmann, B., Sendtner, M.

Notes: The BDNF Emax® System; the NGF Emax® System and the NT-3 Emax® System were each used to quantitate how much of BDNF, NGF and NT-3, respectively, were put into cultures of wildtype and p75NTR-deficient mouse motoneuron cultures. (0186)

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Brain Res. 844, 20-27. Treatment with estrogen and progesterone affects relative levels of brain-derived neurotrophic factor mRNA and protein in different regions of the adult rat brain. 1999

Gibbs, R.B.

Notes: The BDNF Emax® ImmunoAssay System (BDNF ELISA) was used to quantify the levels of BDNF in the left half of each brain. The samples were homogenized in the recommended lysis buffer and acid activated. Excellent detail is provided for the lysis and acidification procedure. The results were reported as nanograms BDNF per gram brain tissue (wet weight). (1101)

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J. Biol. Chem. 273, 29394-29399. Activity-dependent release of brain-derived neurotrophic factor underlies the neuroprotective effect of N-methyl-D-aspartate. 1998

Marini, A.M., Rabin, S.J., Lipsky, R.H., Mocchetti, I.

Notes: The BDNF Emax® ImmunoAssay System was used to determine the levels of BDNF in the conditioned media of primary rat cerebellar neurons. The conditioned media (4ml) was concentrated (200µl) using a Centricon-10 Microconcentrator prior to assay. Cells treated with with N-methyl-D-aspartate released more BDNF than control cells at all time points. After 3 hours of treatment, the granule cells alone released 84.0 ± 9.3pg/ml and the treated cells released 151.3 ± 31. The BDNF released into the media was detectable by Western blotting with an Anti-Human BDNF pAb (antibody attributed to Promega but not mentioned in the Materials and Methods). The cultured granule cells were also assayed for TrkB phosphorylation by incubating with Recombinant Human BDNF. (0733)

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Exp. Neurol. 150, 98-106. Developmental changes in growth factors released by the embryonic inner ear 1998

Bianchi, L.M., Dolnick, R., Medd, A., Cohan, C.S.

Notes: Conditioned media from rat embryonic inner ear cultures was concentrated and analyzed by Western blotting with the Anti-Human BDNF pAb, Anti-Human NT-3 pAb and the Anti-Rat CNTF pAb. The conditioned media and various amounts of the growth factors were blotted together and developed. The Anti-BDNF pAb, Anti-NT-3 pAb and Anti-CNTF pAb were blotted with 50ng and 10ng of the respective factor along with 50ng of each of the other two factors. The Western analysis detected immunoreactivity only for NT-3 in the conditioned media. Some cross reactivity of the Anti-BDNF pAb for NT-3 and the Anti-NT-3 pAb are reported but clearly each has a preference for the respective factor. Cross reactivity to some level can be expected since both are members of the NGF family of growth factors and the proteins were denatured. As expected, no cross-reactivity with CNTF was observed since it is a different class of growth factor more closely related to cytokines. Inner ear and brain homogenates were analyzed with the BDNF Emax® ImmunoAssay System and the NT-3 Emax® ImmunoAssay System. Inner ear homogenates contained 59pg/ml of BDNF and 320pg/ml NT-3. Brain homogenates produced 440pg/ml BDNF and 436pg/ml NT-3. The BDNF Emax®  ImmunoAssay did not recognized NT-3 at levels as high as 600ng/ml and the NT-3 Emax®  ImmunoAssay did not recognize BDNF at levels as high as 1ng/ml. (0026)

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Am. J. Respir. Crit. Care Med. 158 (6), 2002-2005. Neurotrophins are increased in bronchoalveolar lavage fluid after segmental allergen provocation. 1998

Virchow, J.C., Julius, P., Lommatzsch, M., Luttmann, W., Renz, H. and Braun, A.

Notes: Bronchoalveolar lavage fluid samples from human patients were analyzed with the BDNF, NT-3 and NGF Emax® ImmunoAssay Systems. Details of the lavage procedure are provided.  BDNF, NT-3 and NGF data were expressed as an average of duplicates in picograms per milliliter.  (2740)

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