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J. Biol. Chem. 272, 26585-26594. Transcriptional regulation of the human erythroid 5-aminolevulinate synthase gene. Identification of promoter elements and role of regulatory proteins 1997

Surinya, K. H., Cox, T. C., May, B. K.

Notes: Luciferase reporter studies were performed in K562, MEL and COS-1 cells using constructs prepared in the pGL2 Basic Vector. rhTBP was used for gel shift assays. (0322)

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Proc. Natl. Acad. Sci. USA 94, 9717-9722. Transcriptional regulation of the Xlim-1 gene by activin is mediated by an element in intron I 1997

Rebbert, M.L., Dawid, I.B.

Notes: Luciferase reporter constructs, prepared in the pGL2 Basic Vector, were assayed by microinjection into the animal region of two-cell embryos. (0506)

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Blood 89, 2891-2900. Two distinct pathways of interleukin-5 synthesis in allergen-specific human T-cell clones are suppressed by glucocorticoids. 1997

Mori, A., Kaminuma, O., Suko, M., Inoue, S., Ohmura, T., Hoshino, A., Asakura, Y., Miyazawa, K., Yokota, T., Okumura, Y., Ito, K., Okudaira, H.

Notes: Luciferase reporter studies were performed in antigen-specific T cell clones. Experimental constructs were prepared in the pGL2 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. Nuclear extracts were then made from these T cell clones and used for gel shifts with the Gel Shift Assay System. The analysis included the use of the AP-1 Consensus Oligonucleotide, the NF-kappaB Consensus Oligonucleotide and the Oct-1 Consensus Oligonucleotide. (0661)

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J. Biol. Chem. 271, 22746-22753. Identification of a novel cis-acting negative regulatory element affecting expression of the CYP1A1 gene in rat epidermal cells. 1996

Walsh, A. A., Tullis, K., Rice, R. H., Denison, M. S.

Notes: Expression of luciferase reporter constructs, derived from the pGL2-Basic Vector, were studied in rat epidermal keratinocytes. The ProFection® Mammalian Transfection System (calcium phosphate) was used as well. (0194)

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J. Biol. Chem. 271, 15008-15017. Identification of promoter elements involved in cell-specific regulation of rat smooth muscle myosin heavy chain gene transcription. 1996

White, S. L. , Low, R. B.

Notes: Plasmid DNA was purified by the Wizard® Plus Megapreps DNA Purification System. Expression of luciferase reporter constructs derived from the pGL2 Vectors was studied in Primary adult rat aorta, tracheal smooth muscle cells and L8 myoblast cells. (0182)

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J. Clin. Invest. 98(12), 2894-2902. Particle-mediated gene transfer with Transforming Growth Factor β1 cDNAs enhances wound repair in rat skin. 1996

Benn, S., Whitsitt, J., Broadley, K., Nanney, L., Perkins, D., Patel, L., Morgan, J., Swain, W., Davidson, J.

Notes: pGL2 Vector, Beetle Luciferin, Wizard® Plus Maxipreps DNA Purification System, RNasin® Ribonuclease Inhibitor, RQ1 RNase-Free DNase, AMV Reverse Transcriptase and Taq DNA Polymerase were used in this paper. (1963)

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J. Neurobiol. 25, 960-973. Sequencing and promoter analysis of the genomic region between the rat neuronal nicotinic acetylcholine receptor beta 4 and alpha 3 genes. 1994

Boyd, R.T.

Notes: The authors used the Transfectam® Reagent to transfect PC12 cells with plasmids derived from the pGL2 Basic Vector. The Luciferase Assay System was used to measure activity. (1395)

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