We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Citations Search

Sort By:

Neuron 36, 375–386. ProNGF induces p75-mediated death of oligodendrocytes following spinal cord injury. 2004

Beattie, M.S., Harrington, A.W., Lee, R., Kim, J.Y., Boyce, S.L., Longo, F.M., Bresnahan, J.C., Hempstead, B.L. and Yoon, S.O.

Notes: These authors used Western analysis to monitor levels of pro- and mature forms of BDNF and NT-3 from the center of lesions after mouse spinal cord injury. Promega Anti-Human NT-3 pAb and Anti-Human BDNF pAb were used to monitor the mature forms of these neurotrophic factors. (3201)

Expand Full Notes »

J. Neurosci. 23, 5149 – 5160. Endogenously produced neurotrophins regulate survival and differentiation of cortical progenitors via distinct signaling pathways. 2003

Barnabé-Heider, F. and Miller, F.D.

Notes: This paper describes a study of the effect of endogenous neurotrophins on cortical neuron development. Cortical progenitor cells were isolated from mouse embryos and cultured. These precursor cells express both BDNF and NT-3 as well as the corresponding TrkB and TrkC receptors. BDNF and NT-3  signal via Trk receptors to activate the PI3-kinase and MEK pathways. These pathways serve distinct functions, PI3-kinase being essential for progenitor survival and MEK for the differentiation of neurons but not glial cells. The progenitor cell cultures were treated with either Anti-Human BDNF pAb or Anti-Human NT-3 pAb at 20μg/ml to block the function of the endogenous neurotrophins. Anti-ACTIVE® MAPK pAb was used to assess levels of activated MAPK by Western blotting of cell extracts. (2778)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 100(24), 14157-14162. Glatiramer acetate-specific T cells in the brain express T helper 2/3 cytokines and brain-derived neurotrophic factor in situ. 2003

Aharoni, R., Kayhan, B., Eilam, R., Sela, M. and Arnon, R.

Notes: To investigate the activity of glatiramer acetate (GA)-specific T helper cells in the central nervous system, immunohistochemistry was performed on mouse brain sections with a variety of antibodies, including chicken Anti-Human Brain Derived Neurotrophic factor (BDNF) antibody. Mice were perfused with 2.5% paraformaldehyde and brains were dissected and sectioned in 20 micron sections with a Cryostat. After preincubation in PBS with 20% FBS and 0.05% Saponin, 1-10μg/ml Anti-Human BDNF pAb was applied overnight in PBS with 2% FBS and 0.05% Saponin.

Expand Full Notes »

J. Neurosci. 23, 6123-6131. Inhibitory but not excitatory cortical neurons require presynaptic brain-derived neurotrophic factor for dendritic development, as revealed by chimera cell culture. 2003

Kohara, K., Kitamura, A., Adachi, N., Nishida, M., Itami, C., Nakamura, S. and Tsumoto, T.

Notes: To examine the effects of endogenous BDNF expression on different types of neurons, a neuronal co-culture system was established using neurons isolated from BDNF knock-out mice and from mice that were wild type for BDNF but which expressed GFP as a marker. This allowed differentiation between neurons from knock-out and wild-type mice. Uptake of endogenous BDNF from wild-type neurons into knock-out mouse-derived neurons was detectable in co-culture experiments.  Anti-Human BDNF pAb (30μg/ml) was used to neutralize endogenous BDNF in culture.  (2779)

Expand Full Notes »

J. Neurosci. 23, 3761-3770. Long-term depression is not induced by low-frequency stimulation in rat visual cortex in vivo: A possible preventing role of endogenous Brain-Derived Neurotrophic Factor 2003

Jiang, B., Akaneya, Y., Hata, Y., and Tsumoto, T.

Notes: This paper examines the induction of Long Term Depression (LTD) of synaptic transmission in rat visual cortex by Low Frequency Stimulation (LFS).  LTD was not induced by application of LFS in vivo. However,  homosynaptic LTD was induced by LFS when the activity of endogenous BDNF or its receptors was blocked by a drug or antibodies. These results suggest that the LFS-induced form of homosynaptic LTD may not operate in the in vivo cortex, and that endogenous BDNF is a candidate molecule to prevent LFS from inducing synaptic depression.  Anti-Human BDNF pAb was one of the agents used to block endogenous BDNF in rat brain tissue.  A 1μg/μl solution of the antibody was delivered to the cortex via microinfusion pump. Anti-Human BDNF pAb was also used for immunohistochemistry on visual cortex sections. (2780)

Expand Full Notes »

J. Neurosci. 23, 5846-5853. The trophic role of oligodendrocytes in the basal forebrain. 2003

Dai, X, Lercher, L.D., Clinton, P.M., Du, Y., Livingston, D.L., Vieira, C., Yang, L., Shen, M.M. and Dreyfus, C.F.

Notes: The effect of oligodendrocytes on rat neuronal cell cultures was studied. The BDNF and NT-3 in oligodendrocyte conditioned medium (CM) were neutralized by blocking with Anti-Human BDNF and Anti-Human NT-3 pAbs. To determine whether BDNF and NT-3 are components of the CM, solutions containing authentic neurotrophins or conditioned medium solutions were preadsorbed at 4°C for 2 hours with neutralizing antibodies and then exposed to the cultures. Control groups received control IgY. Both anti-BDNF and anti-NT-3 were used at 10 μg/ml.  This concentration was able to fully block BDNF-induced ChAT activity in cultures, and was able to partially block the effects of the conditioned medium for each antibody.  (2826)

Expand Full Notes »

Brain Res. Dev. Brain Res. 121, 97-107. Ethanol-induced alterations in the expression of neurotrophic factors in the developing rat central nervous system. 2000

Heaton, M.B., Mitchell, J.J., Paiva, M., Walker, D.W.

Notes: Brain-derived neurotrophic factor nerve growth factor, neurotrophin-3 levels were quantitated in neonatal and early postnatal rat hippocampus, septum, cortex/striatum and cerebellum using Promega's BDNF Emax® ImmunoAssay System, NGF Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively. (2312)

Expand Full Notes »

Neurology 54, 2009-2011. Increased CSF levels of nerve growth factor in patients with Alzheimer’s disease. 2000

Hock, C., Heese, K., Mueller-Spahn, F., Huber, P., Riesen, W., Nitsch, R.M., and Otten, U.

Notes: The levels of various nerve growth factors were quantitated in the cerebrospinal fluid from Alzheimer's disease patients, nondemented control subjects, and age-matched patients with major depression.  Brain-derived neurotrophic factor, neurotrophin-4, and neurotrophin-3 levels were determined using Promega's BDNF Emax® ImmunoAssay System, NT-4 Emax® ImmunoAssay System, and NT-3 Emax® ImmunoAssay System, respectively. (2317)

Expand Full Notes »

Eur. J. Neurosci. 12, 1891-1899. Reciprocal actions of interleukin-6 and brain-derived neurotrophic factor on rat and mouse primary sensory neurons. 2000

Murphy, P.G., Borthwick, L.A. , Altares, M., Gauldie, J., Kaplan, D., and Richardson, P.M.

Notes: BDNF messages are up regulated when rat dorsal root ganglion cells are treated with IL-6. The IL-6 treatment supports cell survival. Addition of Promega's Anti-Human BDNF pAb to the culture media blocked the survival response to IL-6. (0028)

Expand Full Notes »

J. Neurosci. 20, 2978-2987. Role of brain-derived neurotrophic factor in the circadian regulation of the suprachiasmatic pacemaker by light 2000

Liang, F.-Q., Allen, G., Earnest, D.

Notes: The Anti-BDNF pAb and the Anti-Chicken IgY Alkaline Phosphatase Conjugate were used to produce a 'homebrew' immunoassay. The antibody was used as the detection antibody in a two antibody sandwich assay. (0802)

Expand Full Notes »

J. Neurosci. 20(14), 5367-5373. TrkB receptor ligands promote activity-dependent inhibitory synaptogenesis. 2000

Seil, F.J., and Drake-Baumann, R.

Notes: Mouse Purkinje cell cultures were treated with 50µg/ml Anti-Human BDNF pAb to neutralize BDNF. The cultures had a 1.85 ratio of axosomatic synapse to soma profile after 15 days in vitro as compared to 2.61 for the no-antibody controls. Addition of Anti-Human NT-4 pAb (50µg/ml) with the Anti-Human BDNF pAb further reduced the ratio to 1.35. In the presence of picritoxin, cultures have a 3.46 ratio compared to 2.29 for the control cultures. Addition of both antibodies at 50µg/ml with picritoxin reduced the ratio to 2.49 and at 100µg/ml reduced the ratio to 1.40. (0056)

Expand Full Notes »

FASEB J. 13, 395-410.. A new role for neurotrophins: Involvement of brain-derived neurotrophic factor and neurotrophin-4 in hair cycle control. 1999

Botchkarev, V.A., Botchkarev, N.V., Welker, P., Metz, M., Lewin, G.R., Subramaniam, A., Bulfone-Paus, S., Hagen, E., Braun, A., Lommatzsch, M., Renz, H. and Paus, R.

Notes: Anti-Human BDNF pAb and Anti-Human NT-4 pAb were used for fluorescent immunohistochemical localization of the respective factors to hair follicles of mice. Cryostat sections (8µm thickness) were probed with 1:50 dilutions of the antibodies. Serial sections were also probed with end-labeled probes for BDNF mRNA and TrkB mRNA. The ISH probes were produced using Terminal Deoxynucleotidyl Transferase. (1432)

Expand Full Notes »

Neuroscience 90, 333-347. Alterations in expression of the neurotrophic factors glial cell line-derived neurotrophic factor, ciliary neurotrophic factor and brain-derived neurotrophic factor, in the target-deprived olfactory neuroepithelium 1999

Buckland, M.E., Cunningham, A.M.

Notes: The Anti-Human BDNF pAb, Anti-Rat CNTF pAb and Anti-Human GDNF pAb were used for immunohistochemical analysis of rat olfactory tissue. A lot of detail is provided for the staining. To verify the specificity of the antibodies, the antibodies were immunoabsorbed with their appropriate immunogen and all staining was abolished. (1370)

Expand Full Notes »

J. Neurosci. 19, 3847-3859. Developing Schwann cells acquire the ability to survive without axons by establishing an autocrine circuit involving insulin-like growth factor, neurotrophin-3 and platelet-derived growth factor-BB. 1999

Meier, C., Parmantier, E., Brennan, A.., Mirsky, R. and Jessen, K.R.

Notes: The Anti-Human NT-3 pAb was used to neutralize NT-3 activity in cultures of Schwann cells. The antibody was also used for immunocytochemistry of cultured Schwann cells and nerves of 7day old rats. The Anti-Human BDNF pAb was also used for its BDNF neutralizing activity but the data was not shown. (0675)

Expand Full Notes »

Mol. Cell. Neurosci. 13,  450-64. Endothelial trophic support of neuronal production and recruitment from the adult mammalian subependyma 1999

Leventhal, C., Rafii, S., Rafii, D., Shahar, A., and Goldman S.A

Notes: During brain development endothelial cells may participate in neuronal recruitment, by providing neurotrophic support to new neurons. Levels of  BDNF, NGF, NT-3, and NT-4 mRNAs in cultured adult human brain endothelial cells and human umbilical vascular endothelial cells were determined. The localization of BDNF within these cells was done by immunocytochemistry with the Anti-Human BDNF pAb at a 1:1000 dilution. Cells were fixed with 4% paraformaldehyde with 0.1% glutaraldehyde and permeabilized with  PBS with 0.1% saponin/1% rabbit serum. The Anti-Chicken IgY, HRP Conjugate (1:200 dilution) and a DAB substrate were used to visualize staining. (2392)

Expand Full Notes »

J. Neurosci. 19, 5393-5408. Functionally antagonistic interactions between the TrkA and p75 neurotrophin receptors regulated sympathetic neuron growth and target innervation. 1999

Kohn, J., Aloyz, R.S., Toma, J.G., Haak-Frendscho, M., Miller, F.D.

Notes: Recombinant Human BDNF was used in studies of BDNF neutralization. The Anti-BDNF pAb (> or = 10µg/ml) was found to inhibit 50ng/ml of BDNF from activating autophosphorylation cascades of TrkB in TrkB-expressing NIH3T3 cells. Control IgY at up to 40µg/ml had no inhibitory effect on the autophosphorylation. The Anti-BDNF pAb was added to cultures of primary rat sympathetic neurons to neutralize any BDNF/p75NTR autocrine loop. BDNF causes a decrease in cell proliferation and apoptosis as judged by the CellTiter 96® Assay and the DeadEnd™ Colorimetric Apoptosis Detection System, respectively. The DeadEnd™ System was used in a unique way. Rather than staining the apoptotic nuclei with DAB via the streptavidin-HRP conjugate, a Cy®3-conjugated streptavidin was substituted. To compare the immunolocalization of the tyrosine hydroxylase protein and the p75NTR receptor, serial sections of mouse pineal glands were probed with an anti-tyrosine hydroxylase antibody and the Anti-Human p75 pAb. A lot of detail is provided for tissue processing prior to IHC. The Anti-Human p75 pAb was also used to detect p75 in mouse and rat pineal gland extracts via Western blotting. The name of the DeadEnd™ Colorimetric Apoptosis Detection System has been changed to DeadEnd™ Colorimetric TUNEL System. (0916)

Expand Full Notes »

Science 283, 693-695. Immortal time: Circadian clock properties of rat suprachiasmatic cell lines. 1999

Earnest, D.J., Liang, F.-Q., Ratcliff, M. and Cassone, V.M.

Notes: The Anti-BDNF pAb was used for immunohistochemical localization of 30µm coronal sections of rat brains transplanted with SCN cells. (2019)

Expand Full Notes »

Neuroscience 91(1), 293-303. Neuroprotective effects of brain-derived neurotrophic factor in seizures during development. 1999

Tandon, P., Yang, Y., Das, K., Holmes, G.L., Stafstrom, C.E.

Notes: The Anti-Human BDNF pAb was used to produce a 'homebrew' immunoassay. The antibody was used as the detection antibody in a two antibody sandwich assay for BDNF. Extracts of rat hippocampus previously perfused with sense or antisense oligonucleotides to the BDNF cDNA were assayed. (0027)

Expand Full Notes »

J. Neurosci. 19, 5919-5931. Overexpression of brain-derived neurotrophic factor enhances sensory innervation and selectively increases neuron number. 1999

LeMaster, A.M., Krimm, R.F., Davis, B.M., Noel, T., Forbes, M.E., Johnson, J.E., Albers, K.M.

Notes: The BDNF Emax® ImmunoAssay was used to assess the levels of BDNF in various normal and transgenic mouse tissues: skin, trigeminal ganglia, nodose-petrosal ganglia and SCG. Good detail is provided for tissue preparation. (BDNF ELISA) (0827)

Expand Full Notes »

Exp. Neurol. 150, 98-106. Developmental changes in growth factors released by the embryonic inner ear 1998

Bianchi, L.M., Dolnick, R., Medd, A., Cohan, C.S.

Notes: Conditioned media from rat embryonic inner ear cultures was concentrated and analyzed by Western blotting with the Anti-Human BDNF pAb, Anti-Human NT-3 pAb and the Anti-Rat CNTF pAb. The conditioned media and various amounts of the growth factors were blotted together and developed. The Anti-BDNF pAb, Anti-NT-3 pAb and Anti-CNTF pAb were blotted with 50ng and 10ng of the respective factor along with 50ng of each of the other two factors. The Western analysis detected immunoreactivity only for NT-3 in the conditioned media. Some cross reactivity of the Anti-BDNF pAb for NT-3 and the Anti-NT-3 pAb are reported but clearly each has a preference for the respective factor. Cross reactivity to some level can be expected since both are members of the NGF family of growth factors and the proteins were denatured. As expected, no cross-reactivity with CNTF was observed since it is a different class of growth factor more closely related to cytokines. Inner ear and brain homogenates were analyzed with the BDNF Emax® ImmunoAssay System and the NT-3 Emax® ImmunoAssay System. Inner ear homogenates contained 59pg/ml of BDNF and 320pg/ml NT-3. Brain homogenates produced 440pg/ml BDNF and 436pg/ml NT-3. The BDNF Emax®  ImmunoAssay did not recognized NT-3 at levels as high as 600ng/ml and the NT-3 Emax®  ImmunoAssay did not recognize BDNF at levels as high as 1ng/ml. (0026)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 95, 9614-9619. Neurotrophins induce release of neurotrophins by the regulated secretory pathway 1998

Krüttgen, A., Möller, J.C., Heymach, J.V. Jr, and Shooter, E.M.

Notes: To examine the regulation of neurotrophin secretion, the release of neurotrophins from PC12 cells transfected with various neurotrophin expression vectors was quantitated. To quantitate BDNF an ELISA was developed by coating the plates with the TrkB-Fc receptor body and detecting with the Anti-Human BDNF pAb and the Anti-Chicken IgY, HRP Conjugate. There was no crossreactivity with NGF, NT-3, and NT-4/5 at 1 µg/ml. The Anti-Human p75 pAb was also used in this study. (2359)

Expand Full Notes »

J. Mol. Neurosci. 10, 143-160. Preparation of brain-derived neurotrophic factor- and neurotrophin-3-secreting Schwann cells by infection with a retroviral vector. 1998

Sayers, S.T., Khan, N., Ahmed, Y., Shahid, R. and Khan, T.

Notes: The Anti-BDNF pAb and the Anti-NT-3 pAb were used to demonstrate BDNF and NT-3 expression, respectively, in retrovirally transformed human Schwann cells. The immunocytochemistry was observed with anti-chicken IgY-FITC conjugate. The authors also used the Wizard® Plus Minipreps DNA Purification System for plasmid isolation and Promega restriction enzymes for construction of the retroviral vectors. (0446)

Expand Full Notes »

J. Neurosci. 17, 9492-9505. Activity-dependent dendritic targeting of BDNF and TrkB mRNAs in hippocampal neurons 1997

Tongiorgi, E., Righi, M. and Cattaneo, A.

Notes: Anti-Human BDNF pAb (1:100 dilution) was used to detect BDNF in cultured rat hippocampal neurons before and after treatment with potassium chloride and nocodazole by immunocytochemistry. The antibody was detected with the Anti-Chicken IgY Biotin Conjugate (1:200 dilution). (0276)

Expand Full Notes »

J. Neurosci. 17, 6707-6716. Brain-derived neurotrophic factor enhances long-term potentiation in rat visual cortex. 1997

Akaneya, Y., Tsumoto, T., Kinoshita, S., Hatanaka, H.

Notes:  The involvement of  BDNF and NT-3 in long-term potentiation  in developing rat visual cortex was examined. Recombinant Human NT-3 was assayed for its effects on layer IV sections of the rat visual cortex. The Anti-BDNF pAb was used to detect BDNF in layer IV sections perfused with BDNF immunohistochemistry. (2214)

Expand Full Notes »

Science 276, 1428-1431. Lamina-specific connectivity in the brain: Regulation by N-cadherin, neurotrophins and glycoconjugates. 1997

Inoue, A. and Sanes, J.R.

Notes: The antibody was used to detect the expression of BDNF in transfected QT6 quail fibroblasts by immunocytochemistry. The cells were treated with brefeldin prior to assay. (1581)

Expand Full Notes »

Choose your country


United States

Pacific Asia

Korea, Republic of


United Kingdom