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Citations Search

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J. Biol. Chem. 278(34), 31895-31901. Down-regulation of hypoxia-inducible factor-2 in PC12 cells by nerve growth factor stimulation. 2003

Naranjo-Suárez, S., Carmen Castellanos, M., Alvarez-Tejado, M., Vara,A., Landázuri, M.O. and del Peso, L.

Notes: In this paper, the authors describe use of ImProm-II™ Reverse Transcriptase to transcribe cDNAs for Quantitative RT-PCR. Reverse transcription (RT) reactions were performed with 1μg total RNA from treated rat PC12 cells. Light Cycler reactions were setup with 1-3μl cDNA from the RT reactions. The researchers incubated cells with EGF and bFGF and analyzed HIF-2α mRNA levels. For these experiments, PC12 cells were incubated with 30ng/ml EGF or 50μM bFGF for 8 hours. Erk1&2 phosphorylation was examined by Western blot using the Anti-ACTIVE® MAPK pAb. Western results were visualized by chemiluminesent detection and analyzed with a digital luminescent image analyzer. The Dual-Luciferase® Reporter Assay System was used to analyze PC12 cell-expressed luciferase from pEpoEm1-luc and pEpoE-luc promoter constructs that were normalized with the pRL-TK vector. Cells were harvested 17-18 hours post transfection and treatment. (2725)

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Am. J. Physiol. Cell Physiol. 279, 1024 - 1033. EGF inhibits muscarinic receptor-mediated calcium signaling in a human salivary cell line. 2000

Zhang, B.X., Yeh, C.K., Hymer, T.K., Lifschitz, M.D., and Katz, M.S.

Notes: Promega’s rhIGF-1, rhEGF, and rhTGF-α were added to confluent HSY cell cultures. Carbachol-induced cells were treated with rhEGF (3 ng/ml),  rhTGF-α (3 ng/ml), or rhIGF-I (50ng/ml) for 48 hours before measuring cellular calcium intake with a fura 2-AM dye.  (2786)

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J. Biol. Chem. 273, 22317-22325. Nerve growth factor regulation of m4 muscarinic receptor mRNA stability but not gene transcription requires mitogen-activated protein kinase activity. 1998

Lee, N.H., Malek, R.L.

Notes: The SP6 and T7 RNA Polymerases were used to produce RNA probes for Northern Analysis. The authors examined the half-life of the m4 muscarinic receptor in PC12 cells and transformed PC12 cells following treatment with either murine 2.5S NGF, human recombinant basic FGF or human recombinant EGF. PC12 cells were also treated with the three factors, nuclei isolated, and nuclear run-on transcription assay were performed. (0814)

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Hepatology 27, 1687-1694. Ribavirin inhibits protein synthesis and cell proliferation induced by mitogenic factors in primary human and rat hepatocytes. 1998

Ilyin, G.P., Langouet, S., Rissel, M., Delcros, J.G., Guillouzo, A., Guguen-Guillouzo, C.

Notes: The authors used Recombinant Human EGF to stimulate primary cultures of rat hepatocytes to analyze the effect of ribavirin on proliferation. (0997)

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