Crystal structure of the emerging cancer target MTHFD2 in complex with a substrate-based inhibitor.
Gustafsson, R., Jemth, A.S., Gustafsson, N.M., Färnegårdh, K., Loseva, O., Wiita, E., Bonagas, N., Dahllund, L., Llona-Minguez, S., Häggblad, M., Henriksson, M., Andersson, Y., Homan, E., Helleday, T. and Stenmark, P.
Notes: Human MTHFD2, a mitochondrial methylenetetrahydrofolate dehydrogenase and cyclohydrolase, and the dehydrogenase (D) and cyclohydrolase (C) domain of MTHFD1 were purified from bacteria. To determine the IC50 values of LY345899, MTHFD2 and MTHFD2 reactions proceeded for 15 minutes before NAD(P)H-Glo™ Detection Reagent was added to all wells in the plate, incubated for 60 minutes and luminescence measured. (4961)
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