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Citations Search

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Epub ahead of print. doi: 10.1002/mnfr.201801237. Induction of Autophagy and Activation of SIRT-1 Deacetylation Mechanisms Mediate Neuroprotection by the Pomegranate Metabolite Urolithin A in BV2 Microglia and Differentiated 3D Human Neural Progenitor Cells. 2019

Velagapudi, R., Lepiarz, I., El-Bakoush, A., Katola, F.O., Bhatia, H., Fiebich, B.L., Olajide, O.A.

Notes: In this study, researchers used the Autophagy LC3 HiBiT Reporter Assay to understand the impact of urolithin A on the autophagy pathway, using HEK293, BV2 microglia and differentiated ReNcell VM cells.  (5187)

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Commun. Biol. 1, Article number 54. Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template. 2018

Aird, E.J., Lovendahl, K.N., St Martin, A., Harris, R.S., and Gordon, W.R.

Notes: To properly incorporate chromosomal mutations using the CRISPER/Cas9 system, homology-directed repair (HDR) must be used. Here, a method to increase the efficiency of HDR is presented. The Cas-9 machinery is co-localized to the donor DNA by tethering a single-stranded oligonucleotide to the Cas9-guide RNP. The HiBiT split luciferase is used to monitor HDR effectiveness. The C-terminal 13 amino acids are inserted via CRISPER/Cas-9 and luciferase activity is measured.  (5136)

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ACS Chemical Biology 13(9), 2758–70. Quantitative live-cell kinetic degradation and mechanistic profiling of PROTAC mode of action. 2018

Riching, K.M., Mahan, S., Corona, C.R., McDougall, M., Vasta, J.D., Robers, M.B., Urh, M. and Daniels, D.L.

Notes: The authors use Promega HiBiT and NanoBRET™ technologies to monitor PROTAC-mediated degradation. (5081)

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Virus Res. 243, 69–74. Development of a rapid and quantitative method for the analysis of viral entry and release using the NanoLuc® luciferase complementation assay. 2017

Sasaki, M., Anindita, P.D., Phongphaew, W., Carr, M., Kobayashi, S., Orba, Y. and Sawa, H.

Notes: The authors developed quantitative methods for the detection of cellular entry and release of subviral and flavivirus-like particles (SVPs, VLPs) by tagging the particles with HiBiT. The HiBiT tag was used for quantitation of the particles, and cellular entry was studied using a LgBiT-expressing stable Vero cell line. (4930)

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Biochim. Biophys. Acta 1864, 2322–9. Regions of MRAP2 required for the inhibition of orexin and prokineticin receptor signaling. 2017

Rouault, A.A.J., Lee, A.A. and Sebag, J.A.

Notes: Authors demonstrate use of HiBiT extracellular system to study the role of MRAP2 in GPCR trafficking and surface expression. (4929)

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Biochem. Biophys. Rep. 12, 40–5. Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells. 2017

Oh-Hashi, K., Furuta, E., Fujimura, K. and Hirata, Y.

Notes: The authors used CRISPR/Cas9 editing to tag endogenous ATF4 with HiBiT and then studied changes in abundance following various cell treatments. (4928)

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ACS Chemical Biology Epub before print. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide 2017

Schwinn, M.K., Machleidt, T., Zimmerman, K., Eggers, C.T., Dixon, A.S., Hurst, R., Hall, M.P., Encell, L.P., Binkowski, B.F. and Wood, K.V.

Notes: Using CRISPR/Cas9 gene editing, HiBiT was tagged to the C terminus of HIF1α and several of its downstream transcriptional target proteins. The Nano-Glo® Lytic Detection System was used to quantify HiBiT-tagged endogenous protein levels, and the Nano-Glo® Live Cell Assay System was used for real-time detection in live cells. Bioluminescence was detected using the GloMax® Discover System. This study demonstrated the ability to efficiently tag endogenous proteins with HiBiT, allowing fast and sensitive quantification of the response dynamics in their regulated expression and covalent modifications. (4869)

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