Gao, X., Han, L., Ding, N., Mu, Y., Guan, P., Hu, C., Huang, X.
Notes: Bafilomycin C1 has been shown to have cytotoxic effects specifically against cancer cells. Here, the mechanism of action of Bafilomycin C1 is investigated in human hepatocellular cancer SMMC7721 cells. Bafilomycin C1 shows mitochondrial membrane dysfunction, cell cycle arrest, and down-regulation of cell-cycle regulators. Additionally, bafilomycin C1 treatment lead to induction of apoptotic pathways. In preparation for real-time PCR, total RNA was extracted and cDNA was synthesized from the extracted total RNA using the GoScript™ Reverse Transcription System. Protease Inhibitor Cocktail was used to prepare cell lysates for Western blot analysis. (5086)