Zhang, L., Duan, C.J., Binkley, C., Li, G., Uhler, M.D., Logsdon, C.D. and Simeone, D.M.
Notes: These authors investigated the possible interaction between the TGFß and PKA signaling pathways. Mv1Lu (fetal mink lung) cells or pancreatic acini from male Swiss Webster mice were treated with TGFß1, washed and protein extracted. After determining concentration, equal amounts of protein were used in the SignaTECT® cAMP-Dependent Protein Kinase (PKA) Assay System. The CREB Consensus Oligonucleotide was used in gel shift and supershift assays with nuclear extract prepared from Mv1Lu cells treated with increasing concentrations of TGFß1 to demonstrate the effect of the growth factor on DNA binding. Cell proliferation was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (3114)