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Citations Search

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Mol. Cell. Biol. 24(5), 2169-80. A transforming growth factor beta-induced Smad3/Smad4 complex directly activates protein kinase A. 2004

Zhang, L., Duan, C.J., Binkley, C., Li, G., Uhler, M.D., Logsdon, C.D. and Simeone, D.M.

Notes: These authors investigated the possible interaction between the TGFß and PKA signaling pathways. Mv1Lu (fetal mink lung) cells or pancreatic acini from male Swiss Webster mice were treated with TGFß1, washed and protein extracted.  After determining concentration, equal amounts of protein were used in the SignaTECT® cAMP-Dependent Protein Kinase (PKA) Assay System. The CREB Consensus Oligonucleotide was used in gel shift and supershift assays with nuclear extract prepared from Mv1Lu cells treated with increasing concentrations of TGFß1 to demonstrate the effect of the growth factor on DNA binding.  Cell proliferation was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (3114)

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J. Biol. Chem. 272, 16934-16939. Activating transcription factor 2 (ATF2) down-regulates hepatitis B virus X promoter activity by the competition for the activating protein 1 binding site and the formation of the ATF2-Jun heterodimer 1997

Choi, C. Y. , Choi, B. H. , Park, G. T. , Rho, H. M.

Notes: The AP-1 and CREB Consensus Oligonucleotides were used for gel shift assays with transfected HepG2 cell extracts, E.coli extracts containing eukaryotic transcription factors and a truncated c-jun protein expressed with Rabbit Reticulocyte Lysate. (1343)

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Proc. Natl. Acad. Sci. USA 94, 9487-9492. Characterization of the α1B-adrenergic receptor gene promoter region and hypoxia regulatory elements in vascular smooth muscle. 1997

Eckhart, A.D. , Yang, N. , Xin, X. , Faber, J.E.

Notes: Luciferase studies were performed in aortic and vena caval smooth muscle cells. Transfections were normalized to β-galactosidase activity. Researchers report that pGL3 Promoter and pGL3 Control Vectors were 55-± 3-fold and 340±41-fold greater activity, respectively, than the pGL3 Basic Vector alone. The CREB consensus oligo was used in gel shifts of aortic and vena cava smooth muscle cell nuclear extracts. (1214)

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