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Appl. Plant Sci. 2(10), epub 1400048. Paramagnetic Cellulose DNA Isolation Improves DNA Yield and Quality Among Diverse Plant Taxa. 2014

Moeller, J.R., Moehn, N.R., Waller, D.M. and Givnish, T.J.

Notes: This authors of this study compare isolation of DNA from plants using the Maxwell® 16 System with two other methods on 25 different plant species samples. Researchers started with leaf samples of 25 different species from Wisconsin forests. Nine millimeter punches were taken from each of four individual samples from each species; three replicates per sample were used for each of the three isolation methods the Maxwell® 16 System (paramagnetic cellulose bead); Qiagen DNeasy Plant Mini Kit (silica column) and CTAB-based extraction. The authors evaluated yield, purity and ability to amplify in qPCR and concluded that the Maxwell® 16 system outperformed the other two methods. (The authors also note that Promega provided the Maxwell® 16 Instrument, DNA isolation cartridges and advice on use.) (4515)

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Appl. Plant Sci. 2, apps. 1400048. Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa. 2014

Moeller, J.R. et al.

Notes: The authors evaluated the Maxwell 16 instrument and paramagnetic cellulose particles for the extraction of plant DNA from 25 different plant species. They compared the results to those obtained using Qiagen's DNease plant mini kit, and a CTAB-based method. They found the Maxwell System to be a promising alternative to other technologies for plant DNA isolation.  (4564)

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