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Hum. Gene Ther. 11, 379-388. Deletion of the adenoviral E1b-19kD gene enhances tumor cell killing of a replicating adenoviral vector 2000

Sauthoff, H., Heitner, S., Rom, W.N., Hay, J.G.

Notes: A549 lung adenocarcinoma cells were infected with wildtype adenovirus or a mutant with a deleted E1b-19kD gene. E1b-19kD is a powerful anti-apoptotic agent. Cells infected with the mutant demonstrated more TUNEL positive cells than wildtype or control. The apoptotic effect was heightened with the addition of the chemotherapy drug cisplatin. Excellent detail is provided for cell treatment and preparation. Both adherent and floating cells were affixed to poly-L-lysine coated slides for TUNEL analysis. TUNEL staining was performed with the Apoptosis Detection System, Fluorescein. Update: The Apoptosis Detection System, Fluorescein has been renamed to the DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0444)

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J. Neurosci. 19, 4778-4785. Activation of caspase-3 in the retina of transgenic rats with the rhodopsin mutation S334ter during photoreceptor degeneration. 1999

Liu, C., Li, Y., Peng, M., Laties, A.M., Wen, R.

Notes: The Apoptosis Detection System, Fluorescein, was used to label TUNEL-positive nuclei in 10µm sections of rat retina. A lot of detail is provided for processing of the tissue prior to the TUNEL reaction. The name of the Apoptosis Detection System, Fluorescein, has changed to DeadEnd™ Fluorometric TUNEL System. (0772)

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Eur. J. Neurosci. 11, 769-780. Neuregulin is a mitogen and survival factor for olfactory bulb ensheathing cells and an isoform is produced by astrocytes. 1999

Pollock, G.S., Franceschini, I.A., Graham, G., Marchionni, M.A., Barnett, S.C.

Notes: The Apoptosis Detection System, Fluorescein was used to identify apoptotic primary olfactory bulb ensheathing cells. Cells were purified by FACS with a specific cell surface molecule, then plated onto poly-L-lysine coverslips. Good detail is provided for cell processing for the TUNEL assay. Update: The Apoptosis Detection System, Fluorescein has been renamed as DeadEnd™ Fluorometric TUNEL System (Cat.# G3250). (0539)

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Am. J. Physiol. 277, H1593-H1599. Sustained JNK activation induces endothelial apoptosis: Studies with colchicine and shear stress. 1999

Hu, Y.-L., Li, S., Shyy, J.Y.-J., Chien, S.

Notes: The Apoptosis Detection System, Fluorescein, was used in triple fluorescent labeling. Bovine aortic endothelial cells were treated with colchicine and analyzed for the presence of β-Galactosidase (marker for transfection), TUNEL and nuclei (Hoechst dye). Transfection with a dominant negative mutant JNK (K-R) prevented the appearance of TUNEL staining in the cells and also eliminated the DNA ladder characteristic of apoptosis. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (1025)

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J. Exp. Med. 186, 1749-1756. Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice. 1997

Mizoguchi, A. , Mizoguchi, E. , Smith, R. N. , Preffer, F. I. , Bhan, A. K.

Notes: The Terminal Deoxynucleotidyl Transferase (TdT) enzyme was used to label the fragmented DNA in frozen sections. The DNA was labeled with biotin-dUTP for the TUNEL Assay. (0652)

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